The aim of the experiment was to investigate the efficacy of a smectite-based clay binder (Toxo-MX) in reducing the toxicological effects of aflatoxin B₁ (AFB₁) in commercial broiler chickens. A total of 450 one-day old male broiler chickens were randomly allocated into three treatment groups with ten replicates of 15 birds each in a 42-day feeding experiment. The dietary treatments included a negative control (NC, a basal diet with no AFB₁ and binder), a positive control (PC, a basal diet contaminated with 500 ppb of AFB₁) and a smectite-based mycotoxin binder(Toxo-MX, PC with smectite clay binder). AFB₁ challenge resulted in 14 to 24% depression in growth performance, elevated levels of aspartate aminotransferase (AST), and gamma-glutamyl transferase (GGT), organ enlargement and immuno-suppression.As compared to PC, feeding of Toxo-MX improved the final weight (15%; p < 0.0001), average daily gain (ADG) (15%; p < 0.001) and feed efficiency of broilers (13%; p < 0.0003) but did not have any effects on liver enzyme activities. Supplementation of smectite claysignificantly increased serum globulin levels and reduced the weight of the liver (p < 0.05) as compared to AFB₁-fed broiler chickens. The severity of lesions (inflammatory and degenerative changes) observed in the liver, kidney, heart, pancreas, and lymphoid organs in PC birds was reduced by feeding smectite clay. The immuno-suppression caused by AFB₁ was moderately ameliorated in Toxo-MX groupby stimulating the production of antibodies against IBD at day 42 (p < 0.05). In conclusion, dietary supplementation of a smectite-based mycotoxin binder to the diet containing AFB₁ improved growth performance, reduced toxicological effects in liver and improved humoral immune response in broilers, suggesting its protective effect against aflatoxicosis.

Yeast cell wall-based preparations have shown efficacy against Aspergillus-based toxins but have lower impact against type-B trichothecenes. Presently, we investigated a combination of deoxynivalenol (DON), T-2 toxin (T2) and zearalenone (ZEA), and the effect of a yeast cell wall extract (YCWE) and a post-biotic yeast cell wall-based blend (PYCW) with the objectives of preventing mycotoxins’ negative effects in commercial broilers. A total of 720 one-day-old male Cobb broilers were randomly allocated to: (1) control diet, (aflatoxins 6 µg/kg; cyclopiazonic acid 15 µg/kg; fusaric acid 25 µg/kg; fumonisin B1 310 µg/kg); (2) Diet1 + 0.2% YCWE; (3) Diet1 + 0.2% PYCW; (4) Contaminated diet (3.0 mg/kg DON; 2.17 mg/kg 3-acetyldeoxynivalenol; 104 g/kg T2; 79 g/kg ZEA); (5) Diet4 + 0.2% YCWE; and (6) Diet4 + 0.2% PYCW. Naturally contaminated diets adversely affected performance, serum biochemistry, liver function, immune response, altered cecal SCFA goblet cell count and architecture of intestinal villi. These adverse effects were reduced in birds fed PYCW and to a lesser extent YCWE, indicating protection against toxic assault. PYCW yielded better production performance and stimulated liver function, with higher response to NDV and IBV vaccination. Furthermore, mycotoxins were found to affect production outputs when evaluated with the European poultry production efficiency factor compared to control or YCWE and PYCW supplemented treatments. Taken together, YCWE, when complemented with nutritional add-ons (PYCW), could potentiate the remediation of the negative effects from a multi mycotoxins dietary challenge in broiler birds.

The effect of selenium yeast and its combination with sodium selenite on laying hens’ production performance, egg quality, and selenium enrichment were evaluated in this experiment. Dual purpose Giriraja (200) were randomly divided into 4 groups having 5 replicates of 10 (9 females+1 male) birds each. The treatment groups included T1 (control diet without selenium), T2 (control diet + sodium selenite @ 0.5mg/kg diet), T3 (control + sodium selenite @ 0.3mg/kg diet + selenium yeast @ 0.2mg/kg diet) and T4 (control diet+ selenium yeast @ 0.5mg/kg diet). The experiment was for 8 weeks starting from 30 weeks of age. 150 g feed per bird every day and 16h light schedule was followed. Egg production performance, egg quality parameters, and egg selenium deposition were recorded. Supplementation of different sources of selenium alone and their combination did not show significant difference in production performance viz., weekly hen housed egg production and feed conversion ratio per dozen eggs and also egg quality parameters. Significantly higher (P<0.05) egg selenium content was observed in the selenium yeast supplemented group in the fourth and eighth weeks of the trial. In the eighth week, the combination group's egg selenium content was significantly greater (P<0.05) than that of the sodium selenite supplemented group. In conclusion, selenium supplementation was not beneficial to production performance as selenium present in the feed raw material was sufficient to meet the requirement of layer (0.05–0.08mg/kg diet). Nevertheless, addition of selenium was useful to fortify the egg. Though combination showed significant improvement compared to inorganic but the deposition was not as efficient as organic alone. Hence, the addition of organic sources alone is a better option to produce selenium enriched eggs.