This paper proposes a methodology for plant root system and soil moisture analysis through the geoelectrical prospecting method. Overall, bench and field experiments are implemented to analyze the behavior of electrical conductivity of the soil in relation to moisture content and plant root system growth. Specifically, Wenner array and lateral profiling technique are used to stratify the soil in horizontal layers, performing in-depth analysis. Millet (Pennisetum glaucum L.), bean (Phaseolus vulgaris L.) and sorghum (Sorghum bicolor L. Moench) are used to analyze the root system behavior. Results show that the soil water dynamics can be observed through soil stratification in horizontal layers and the plant root system is correlated with apparent electrical conductivity of the soil. Thus, geoelectric prospecting methods can be used as an analysis tool, both of soil moisture dynamics and of plant roots, to support decision making regarding soil and crop management.