Lime is an important commercial product in tropical and subtropical regions, where drought stress is becoming one of the most severe environmental challenges in the agricultural sector. Melatonin is an antioxidant molecule that helps plants regulate their development and respond to a variety of stresses. In this research, the effects of exogenous melatonin treatments were evaluated at different concentrations (0, 50, 100, and 150 μM) on biochemical aspects and gene expression in two species of lime plants (“Mexican lime” and “Persian lime”) under normal (100% field capacity (FC)) and drought stress conditions (75% and 40% FC). The experiments were factorial and based on a completely randomized design (CRD) with four replicates. Drought stress caused electrolyte leakage (EL) as well as accumulations of hydrogen peroxide (H₂O₂) and malondialdehyde (MDA), indicating the occurrence of damage to cellular membranes. In contrast, the melatonin pretreatment at various concentrations reduced the levels of EL, H₂O₂ and MDA while mitigating the negative effects of drought stress on the two lime species. The application of melatonin (100-μM) significantly increased the level of proline content and activity of antioxidant enzymes in plants under drought stress compared to control plants. According to real-time PCR analysis, drought stress and melatonin treatment enhanced the expression of genes involved in ROS scavenging, proline biosynthesis, and cell redox regulation in both species, as compared to their respective controls. According to these findings, melatonin is able to detoxify ROS and regulate antioxidant systems, thereby protecting lime plants from drought stress-induced damages.

Bermudagrass (Cynodon spp.) is a major turfgrass for home lawns, public parks, golf courses and sport fields and is known to have originated in the Middle East. Morphological and physiological characteristics are not sufficient to differentiate some bermudagrass genotypes because the differences between them are often subtle and subjected to environmental influences. In this study, twenty seven bermudagrass accessions and introductions, mostly from different parts of Iran, were assayed by inter-simple sequence repeat (ISSR) markers to differentiate and explore their genetic relationships. Fourteen ISSR primers amplified 389 fragments of which 313 (80.5%) were polymorphic. The average polymorphism information content (PIC) was 0.328, which shows that the majority of primers are informative. Cluster analysis using the un-weighted paired group method with arithmetic average (UPGMA) method and Jaccard’s similarity coefficient (r = 0.828) grouped the accessions into six main clusters according to some degree to geographical origin, their chromosome number and some morphological characteristics. It can be concluded that there exists a wide genetic base of bermudograss in Iran and that ISSR markers are effective in determining genetic diversity and relationships among them.