This paper reports a novel green approach for the synthesis of silver nanoparticles (AgNPs) using aqueous seed extract of Dracocephalum moldavica (L.) under ambient conditions. Processes such as Ultraviolet-visible (UV-vis) spectrometer, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), energy dispersive X-ray analysis (EDX), and transmission electron microscopy (TEM) were carry out to characterize AgNPs. The presence of AgNPs in the prepared solution was approved by a peak to occur at 443 nm. XRD pattern indicated the crystalline structure of the nanoparticles (NPs) while the FTIR spectra confirm the attendance of plant residues adsorbed by these NPs. TEM images revealed a near spherical shape of these NPs, and EDX provided the expected elemental composition. The synthesized AgNPs showed excellent antimicrobial activities against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Serratia marcescens, Staphylococcus epidermidis and Bacillus subtilis.