Gerbera (Gerbera hybrida) is a widely cultivated ornamental plant. However, its genetic improvement is limited by the lack of genetic analysis and molecular markers for traits. In this study, we analyzed the phenotypic and genotypic variation of 140 F₁ progeny from two gerbera varieties with different flower types and colors. We evaluated the flower’s morphology, color, and pigment content of the F₁ population and performed cluster principal component analysis (PCA) and correlation analysis. The results showed that the main ornamental traits of the hybrid progeny varied greatly. The segregation ratios of single and double flowers and ligulate and split ray florets were both 1:1. The flower colors of the F₁ progeny were mainly red and purple-red, similar to the male parent’s color. Furthermore, we conducted a genetic analysis of the hybrid progeny using EST-SSR markers and performed association analysis with phenotypic traits. We identified 2, 2, 3, 1, and 2 loci to be associated with peduncle length (PL), ray floret length (RFL), and outer ray floret; the level of apex relative to the top of involucre (LAI); outer corolla lips (OCL); and the b* of ray floret color, respectively. Our results reveal the genetic patterns of important ornamental traits and provide a theoretical basis and practical tools for gerbera genetic breeding.

Light is an important environmental signal that governs plant growth, development, and metabolism. Constitutive photomorphogenic 1 (COP1) is a light signaling component that plays a vital role in plant light responses. We isolated the COP1 gene (LoCOP1) from the petals of Lilium ‘Siberia’ and investigated its function. The LoCOP1 protein was found to be the most similar to Apostasia shenzhenica COP1. LoCOP1 was found to be an important factor located in the nucleus and played a negative regulatory role in floral scent production and emission using the virus-induced gene silencing (VIGS) approach. The yeast two-hybrid, β-galactosidase, and bimolecular fluorescence complementation (BiFC) assays revealed that LoCOP1 interacts with LoMYB1 and LoMYB3. Furthermore, light modified both the subcellular distribution of LoCOP1 and its interactions with LoMYB1 and MYB3 in onion cells. The findings highlighted an important regulatory mechanism in the light signaling system that governs scent emission in Lilium ‘Siberia’ by the ubiquitination and degradation of transcription factors via the proteasome pathway.

The calamondin (Citrus × microcarpa) is highly valued for its ornamental appeal and rich aromatic compounds, making it suitable for therapeutic gardens and widely applicable in the cosmetics, food, pharmaceutical, and perfume industries. Despite its importance, there is a lack of research on its floral volatiles. This study utilized headspace solid-phase microextraction gas chromatography–mass spectrometry (HS–SPME–GC–MS) to detect the volatile organic compounds (VOCs) of calamondin at different floral developmental stages: bud (BS), half-open (HS), full bloom (FS), and senescence (SS). Multivariate statistical analysis was employed to elucidate the aromatic characteristics. The results identified 67 VOCs across the four stages, including forty-eight terpenoids, six esters, five aromatics, four aldehydes, one olefin, one alcohol, and two others. Thirty-three VOCs were common across all stages, while BS, HS, FS, and SS had three, three, four, and nine unique VOCs, respectively. The total VOC content increased initially and then decreased as the flowers developed, with terpenoids being the predominant compounds, accounting for over 90% of the total emissions at all stages. Principal component analysis and hierarchical cluster analysis confirmed significant differences in VOC profiles at different stages. Partial least squares discriminant analysis identified five VOCs with variable importance in projection (VIP) values greater than one, including limonene, linalool, β-pinene, germacrene D, and β-ocimene, indicating their varying emission levels across stages. These findings enhance our understanding of the VOC characteristics of calamondin flowers and provide a scientific basis for further ornamental and industrial applications.