The spatial distributions of nitrogen (N) and phosphorus (P) in surface sediments are of great significance in studying the ecological process of nutrient cycling in intertidal flats. However, little is known about N and P’s spatial distribution in intertidal flats of the Yellow River Delta (YRD). We analyzed the N and P contents in surface sediments and Suaeda glauca density at the low-tidal level to identify the spatial distributions of nutrients and their influencing factors in coastal tidal flat sediments. The results showed that the total nitrogen (TN) and total phosphorus (TP) concentrations in this study were both lower than the background values of China’s shallow sea sediments. The spatial distributions of N and P had significantly spatial heterogeneity, while those of the nutrients at different distances from the low-tidal level to the coastline showed no significant distance effects. The spatial distribution of S. glauca in coastal tidal flats had significant location characteristics and was closely related to the distribution of TN and pH. The TN in non-estuarine intertidal flats was less than that in estuaries; in contrast, the TP was higher in non-estuaries. There are some differences of N and P between estuary and non-estuary areas.

Aim: Road networks are common landscape disturbances that can facilitate the spread of invasive plants. This study explored the influence of distance from the road, habitat type and broader environmental factors in shaping the distribution patterns of both invasive and native species in the above‐ground vegetation and soil seed banks.Location: Guangxi, China.Methods: We collected data on plant species composition from both soil seed banks and above‐ground vegetation at six distances from the road edge: 0 m, 2 m, 4 m, 9 m, 14 m and 24 m in three habitat types, including abandoned land, Eucalyptus plantations and natural secondary forests. We collected data on environmental variables at each sampling location. We examined the compositional similarity of plant communities by non‐metric multidimensional scaling (NMDS) and identified the influence factors by redundancy analysis (RDA).Results: Our results indicated that invasive species richness decreased with distance from the road, especially in natural secondary forests. Conversely, native species did not show consistent distribution patterns relative to distance from roads across the various habitats. The composition of invasive plant communities was similar in both soil seed banks and above‐ground vegetation, while only 13.33% of native species identified in the soil were observed in the above‐ground vegetation. Road characteristics, human disturbance and soil properties correlated with the distribution of invasive and native species, with the strength of these correlations varying among habitat types. The richness and density of native plants were associated with the presence of invasive alien plants at various distances from the road across the three types of habitats.Main Conclusions: The study highlights that proximity to the road, habitat type and environmental factors are critical in determining the distribution of plant species within nature reserves. Moreover, it underscores the importance of integrating both above‐ground and seed bank perspectives for effective management strategies to control invasive species and promote native plant communities.

Genetic variation and population structure may reflect important information for invasion success of exotic plant species and thus help improve management of invasive plants. Spartina alterniflora is an invasive plant that is a major threat to the economy and environment of the coastal regions in China. We analyzed the genetic structure and diversity of six populations of S. alterniflora differing in invasion histories in Guangxi, China. A total of 176 individuals from the six populations produced 348 AFLP fragments. The average heterozygosity was significantly lower than in the native population. And genetic bottlenecks were also detected in most populations. Standardized F_ST statistics (Φpt = 0.015) and AMOVA results indicated weak genetic differentiation. Genetic admixture and obviously isolation by distance indicated populations in Guangxi come from a pre-admixed population by a single introduction. High phenotypic variations of S. alterniflora in Guangxi influenced by soil salinity and temperature might be an important reason for the successful invasion.

In order to understand the spatial distribution, ecological risks, and pollution status of nutrients and heavy metals in the coastal tidal creek water of the Yellow River Delta (YRD), a total of 21 water samples were collected from 7 sample sites. The results indicated that along the coastline from northwest to southeast in the YRD, the concentrations of TN, TP, and NH₄⁺-N in the water decreased and then increased; the Cu concentration increased, decreased, and then increased; and the Pb concentration decreased. The average TN/TP mass ratio indicated that the tidal creek water belonged to a potential phosphorus-restricted eutrophication state. The RI result indicated that Cu and Pb in the water were at low ecological risk, while the SSD and RQ results indicated that Cu in the water was at a high ecological risk level and had potential harm to aquatic organisms. Based on the single-factor method, the water quality of the tidal creek inside and outside the Yellow River Delta Nature Reserve belonged to Grade IV. Cu should be controlled to improve the water quality and reduce the ecological risk, especially in the Yellow River Delta Nature Reserve.

Biological invasions have become one of the greatest threats to global biodiversity and ecosystem conservation. Most previous studies have revealed how successful invasive species adapt to new environments and climate change through phenotypic and genetic evolution. Some researchers suggested that understanding unsuccessful or less successful biological invasions might be important for understanding the relationships between invasion adaptability and climate factors. We compared the sexual reproduction ability, genetic diversity, and gene × environment interaction in two intentionally introduced alien species in China (Spartina anglica and Spartina alterniflora) based on restriction site-associated DNA (RAD) sequencing. After more than 50 years, the distribution of S. alterniflora has rapidly expanded, while S. anglica has experienced extreme dieback. A total of 212,939 single nucleotide polymorphisms (SNPs) for the two Spartina species were used for analysis. The multilocus genotype (MLG) analysis revealed that clonal reproduction was the prevalent mode of reproduction in both species, indicating that a change in the mode of reproduction was not the key factor enabling successful invasion by Spartina. All genetic diversity indicators (He, Ho, π) in S. alterniflora populations were at least two times higher than those in S. anglica populations, respectively (p < 0.001). Furthermore, the population genetic structure and stronger patterns of climate-associated loci provided support for rapid adaptive evolution in the populations of S. alterniflora in China. Altogether, our results highlight the importance of genetic diversity and local adaptation, which were driven by multiple source populations, in increasing the invasiveness of S. alterniflora.