Papaya (Carica papaya) is one of the plants that represent a major source of bioactive substances that are cheaper and non-toxic, such as antibiotics. It is globally used as a supplement diet for positive effects in pharmacological activities. In this regard, the current study sheds light on the possible roles of papaya seed methanolic extract (PSE) as a dietary supplement to improve growth performance, sexual maturity, carcass composition, immunity responses, histological structure of gonads, and anti-pathogenic activity (Aeromonas hydrophila) at the end of a feeding trial extend for ten days of Nile tilapia, with special reference to its potential effect as resistance against parasitic (Cichlidogyrus tilapiae). Four experimental groups with four replications of Nile tilapia fry (0.5 ± 0.1 g as 20 fish per replication) were fed (0, 0.5, 1.0, and 2.0 g/kg PSE in diets) for 20 weeks. Results showed that fish dietary supplementation with PSE had a significantly positive (P < 0.05) effect on growth performance, feed utilization, digestive enzyme, carcass composition, antioxidant, immunity responses, and hematological and biochemical indices, especially PSE 2.0 g/kg than control diet. Furthermore, PSE had a positively affected fish mortality after injection with A. hydrophila and in vitro positive efficacy against C. tilapiae. Fish fed with dietary PSE increased the sex ratio in favor of male fish by achieving a 77% male phenotype. Conclusively, this study declared that PSE extract, specifically 2 g/kg, could enhance growth and health through the modulation of innate immunity and a positive effect against disease in fish. Additionally, it reduced the reproductive performance of Nile tilapia by reducing GSI, which subsequently affected the gonadal histology leading to infertility.

Despite wide studies of biomonitoring aquatic environment through dynamics of host–parasite interaction, bio-indicators to track the influence and accumulation of heavy metals on fish are still few. The present study sheds light on the relation between fish parasites and heavy metals as it threatens fish’s health and, as a consequence, that of humans after fish consumption. Samples of Nile tilapia (Oreochromis niloticus) were collected in Burullus Lake, a wild fish source, and from a private fish farm in Kafr El-Sheikh Governorate, in Egypt. They were exposed to various pollutants associated with anthropogenic activities to determine the levels of accumulation of Fe, Zn, and Cu, along with the top three most toxic metals (As, Cd, and Pb) in water and fish tissues of gills, intestine, liver, and muscles in both wild and farmedO. niloticus. The results showed the order of abundance: Fe < Zn < Pb < Cu < As < Cd. In waters of both farmed and wild fish, there was a significant negative relation between parasite prevalence and heavy metals, including Zn, Pb, and As. Also, there was a significant positive relation between parasite prevalence with Cu while no significant relation was found with Fe and Cd. Heavy metal content was significantly higher (p> 0.05) in non-infected than infected farmed and wildO. niloticus. In addition, a significantly decreased concentration (p> 0.05) of essential heavy metal was recorded in wild fish compared to farmed specimens, while non-essential heavy metal was significantly higher (p> 0.05) in wild compared to farmed fish. Bioaccumulation factors (BAF) of different organs ofO. niloticuswere ranked in ascending order: Liver > Gills > Intestine > Muscles. In general, the risk assessment showed safe human consumption of farmed and wild fish under the reported environmental conditions in this study. Moreover, the parasite's presence can be adopted as a surrogate indicator to estimate the potential impact of heavy metal pollution and accumulation.

This study aimed to evaluate the effect of adding liquid extract of algae (Hypnea musciformis, Grateloupia acuminata, and Sargassum muticum) (HGS) and Magnesium oxide nanoparticles (MgO NPs) using this extract to rear water of Oreochromis niloticus, on improving culture water indices, growth performance, digestive enzyme, hemato-biochemical characters, immune, antioxidative responses, and resistance after challenged by Aeromonas hydrophila with specific refer to the potential role of the mixture in vitro as resistance against three strains bacteria (Aeromonas sobria, Pseudomonas fluorescens, P. aeruginosa) and one parasite (Cichlidogyrus tilapia). The first group represented control, HGS0, whereas the other group, HGS5, HGS10, and HGS15 mL^-1 of liquid extract, as well as all groups with 7.5 μg mL^-1 MgO-NPs added to culture water of O. niloticus, for 60 days. Data showed that increasing levels at HGS 10 and HGS15 mL^-1 in to-culture water significantly enhanced growth-stimulating digestive enzyme activity and a significantly improved survival rate of O. niloticus after being challenged with A. hydrophila than in the control group. The total viability, coliform, fecal coliform count, and heavy metal in muscle partially decreased at HGS 10 and HGS15 mL^-1 than in the control group. Correspondingly, the highest positive effect on hemato-biochemical indices was noticed at levels HGS 10 and HGS15 mL^-1. Fish noticed an improvement in immune and antioxidant indices compared to control groups partially at HGS 10 and HGS15 mL^-1. Interestingly, fish cultured in rearing water with the mixture provided downregulated the related inflammatory genes (HSP70, TNF, IL-1β, and IL-8) partially at HGS15 mL^-1. In vitro, the mixture showed positive efficiency as an antibacterial and partially antiparasitic at HGS 10 and HGS15 mL^-1. This study proposes utilizing a mixture of (HGS) and (MgO-NPs) with optimum levels of 10-15 mL^-1 in cultured water to improve water indices, growth, health status, and increased resistance of O. niloticus against bacterial and parasitic infection.

Medicinal plants are a potential safe source of bioactive compounds. Fish diet supplemented with the medicinal plant bottle gourd (Lagenaria siceraria) seed powder was evaluated in this study for the potential effect on growth performance, antioxidative status, immunological response, and resistance to infectious pathogens in the Nile tilapia (Oreochromis niloticus). Nile tilapia fingerlings with mean weight ( ± SD)= (25.64 ± 0.17 g), were fed four L. siceraria seed supplemented diets (LSSD) at 0.0, 1, 2, and 3% for 60 days. Specimens were then challenged with the bacterium Aeromonas hydrophila for 10 days. Also, three different concentrations (2.5, 5, and 10 ml/L) of Lagenaria siceraria ethanolic extract (LSEE) were tested for their antibacterial and antiparasitic efficacy on four selected bacterial and one parasitic species. All parameters’ values generally improved with elevating the content of the L. siceraria seed powder in the diet. Dietary administration of LSSD-3% enabled significant (P &lt; 0.05) higher growth performance, and feed utilization efficiency. It reduced the mortality induced by A. hydrophila infection, increased crude protein content in the fish body and exhibited the highest in vitro antibacterial and antiparasitic efficiency. RBCs, WBCs, Hb, PCV, MCV, MCH, and total serum protein values in pre- and post-challenge groups were significantly higher (P &lt; 0.05) in the LSSD-3% group. While MCHC, ALT, AST and glucose levels were significantly lower (P &lt; 0.05) than those of the other groups. Lysozyme and antioxidant enzyme activities in pre- and post-challenge groups were also higher (P &lt; 0.05) in the LSSD-3% group compared to the other groups. LSEE provided good efficacy against Gram-negative bacterial strains, mild efficacy against Gram-positive bacterial strains, and an antagonistic effect on the parasite Cichlidogyrus Tilapiae. The 10 ml/L concentration was the most effective against the pathogens followed by the 5 ml/L concentration and then 2.5 ml/L. Our findings suggest the feasibility of supplementation of Nile tilapia (O. niloticus) diet with L. siceraria seed powder by 3% to improve the growth performance, immunity, and vital parameters.

Elements accumulation in crayfish is proportional to the increase in bioavailability (direct contact) with the surrounding water, sediment, and feeding. Five heavy metals (Cu, Cr, Mn, Ni, and Ag) and lithium (Li) were analyzed in the sediment, water, and crayfish tissues. Elements (heavy metals and lithium) concentrations in sediment, water, and crayfish tissues showed significant differences between the two sampling stations (El-Qanatir and El-Rahawi drain). However, the levels of elements in crayfish tissues were arranged in declining order as hepatopancreas > gills > exoskeleton > muscles for Cu and Cr; hepatopancreas > exoskeleton > gills > muscles for Ni and Ag; and exoskeleton > gills > hepatopancreas > muscles for Li and Mn. The human health hazard evaluation of heavy metals and lithium exposure via edible tissue consumption was assessed for both children and adult consumers. The target hazard quotient THQ values of crayfish edible tissues (less than 1) will not impose any health implications for consumers who ingest edible tissues in sufficient quantities. Furthermore, the hazard index (HI) values reported for children and adult consumers were lower than one, indicating non-carcinogenic and carcinogenic hazards, suggesting that crayfish edible tissues are safe for human ingestion. This evidence also found that Procambarus clarkii could be a good bio-indicator organism for monitoring potentially metals in aquatic systems.