In response to increasing natural surfactant demand and environmental concerns, natural plant-based surfactants have been replacing synthetic ones. Saponins belong to a class of plant metabolites with surfactant properties that are widely distributed in nature. They are eco-friendly because of their natural origin and biodegradable. To date, many plant-based saponins have been investigated for their surface activity. An overview of saponins with a particular focus on their surface-active properties is presented in this article. For this purpose, works published in the past few decades, which report better surfactant relevant properties of saponins than synthetic ones, were extensively studied. The investigations on the potential surfactant application of saponins are also documented. Moreover, some biological activities of saponins such as antimicrobial activity, antidiabetic activity, adjuvant potentials, anticancer activity, and others are reported. Plants rich in saponins are widely distributed in nature, offering great potential for the replacement of toxic synthetic surfactants in a variety of modern commercial products and these saponins exhibit excellent surface and biological activities. New opportunities and challenges associated with the development of saponin-based commercial formulations in the future are also discussed in detail.

In this study, saponins extracted from leaves and stem bark of Jatropha curcas L. were investigated for surface-active properties. Conductivity and surface tension measurements revealed the micellar character of J. curcas saponin, with the average CMC, determined to be 0.50 g/L and 0.75 g/L for leaf and stem bark saponin, respectively. Stem bark saponin reduced the surface tension of water to a greater extent (${\gamma }_{\mathrm{C}\mathrm{M}\mathrm{C}}=$ 37.65 mN/m) compared to leaf saponin (${\gamma }_{\mathrm{C}\mathrm{M}\mathrm{C}}=$ 49.27 mN/m) indicating its efficient surface activity and potential detergency. pH measurement confirmed the weakly acidic nature of saponin with a pH value lying slightly below the range suitable for hair and skin. Stem bark saponin showed better cleaning ability, foaming ability and foam stability than leaf saponin, due to a sufficient reduction in the surface tension of water. The results obtained suggest that the saponin extracted from both the leaves and stem bark of J. curcas can be used as environmentally friendly alternatives to synthetic surfactants.

We investigated the hydration behavior of dipalmitoylphosphatidylcholine (DPPC) bilayers containing sodium β-sitosteryl sulfate (PSO₄). PSO₄ was found to enhance hydration in the headgroup region of DPPC bilayers. Therefore, with the incorporation of PSO₄ into DPPC membranes, the amount of water required to reach the fully hydrated state was enhanced as indicated by the constant values of the main phase transition temperature (T_m) and the bilayer repeat distance (d). For example, with the addition of 20 mol% of PSO₄, the saturation point was shifted to ~70 wt% water compared to ~40 wt% for pure DPPC and 47 wt% for DPPC-cholesterol. The effectiveness of PSO₄ in fluidizing the membrane and enhancing its hydration state can be useful in the pharmaceutical and cosmetic industries.

The size, dispersibility, and fluidity of DPPC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine), POPC (1-palmitoy-2-oleoyl-sn-glycero-3-phosphocholine), and DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine) liposomes doped with β-sitosteryl sulfate (PSO₄) were comparatively studied. In all three types of liposomes, PSO₄ reduced sizes and enhanced the negative values of the ζ-potential. However, the effect on sizes quantitatively differed in the three cases in a manner dependent on their phase behaviors. PSO₄ rigidified each type of membrane in its liquid crystalline phase and fluidized the gel phase. It enhanced the glucose trapping efficiency (TE) of both DPPC and DOPC liposomes. The TE of DPPC first increased with the increasing concentration of PSO₄, then decreased gradually. On the other hand, in the case of DOPC, the TE increased significantly upon addition of PSO₄, then remained nearly constant. Though the exact dependence of TE on the PSO₄ concentration differed in the two cases, its effect, in each case, was more than the effect of β-sitosterol (POH). The ability of PSO₄ for reducing the size and enhancing dispersibility and TE of liposomes can be useful for preparing cosmetics and pharmaceutical formulations.