Accurate estimation of above-ground biomass (AGB) is vital for carbon accounting, biodiversity conservation, and sustainable forest management, especially in tropical regions under strong anthropogenic pressure. This study estimated and mapped AGB in the Atacora Mountain Chain, Togo, using a multi-source remote sensing approach within Google Earth Engine (GEE). Field data from 421 plots of the 2021 National Forest Inventory were combined with Sentinel-1 Synthetic Aperture Radar, Sentinel-2 multispectral imagery, bioclimatic variables from WorldClim, and topographic data. A Random Forest regression model evaluated the predictive capacity of different variable combinations. The best model, integrating SAR, optical, and climatic variables (S1S2allBio), achieved R2 = 0.90, MAE = 13.42 Mg/ha, and RMSE = 22.54 Mg/ha, outperforming models without climate data. Dense forests stored the highest biomass (124.2 Mg/ha), while tree/shrub savannas had the lowest (25.38 Mg/ha). Spatially, ~60% of the area had biomass ≤ 50 Mg/ha. Precipitation correlated positively with AGB (r = 0.55), whereas temperature showed negative correlations. This work demonstrates the effectiveness of integrating multi-sensor satellite data with climatic predictors for accurate biomass mapping in complex tropical landscapes. The approach supports national forest monitoring, REDD+ programs, and ecosystem restoration, contributing to SDGs 13, 15, and 12 and offering a scalable method for other tropical regions.