The effects of Pseudomonas putida, Pseudomonas alcaligenes and a Pseudomonas isolate (Ps28) on the hatching and penetration of Meloidogyne incognita in chi

Bacterial and fungal diseases of tomato caused severe yield losses and are important constrains in successful cultivation. Therefore, impact of zinc oxide nanoparticles (ZnO NPs) on growth of tomato (Solanum lycopersicum L.) and bacterial (Pseudomonas syringae pv. tomato, Xanthomonas campestris pv. vesicatoria, Pectobacterium carotovorum subsp. carotovorum and Ralstonia solanacearum) and fungal diseases (Fusarium oxysporum f. sp. lycopersici and Alternaria solani) were assessed. Maximum increase in plant growth (27.8–35.8% increase in shoot dry weight), photosynthetic pigments and proline content and reduction in disease indices (up to 1) were observed by foliar application of 0.20 mL⁻¹ NPs followed by seed priming. Use of 0.10 mL⁻¹ NPs was less effective. Scanning electron microscopy indicated adverse effect of NPs on pathogens. Higher accumulation of Zn occurred in leaves treated with NPs. Substantial management of above mentioned bacterial and fungal diseases may be obtained by foliar application of 0.20 mL⁻¹ ZnO NPs.

Nanoparticles (NPs) have been applied for management of pathogenic microbes affecting agricultural crops. Pectobacterium betavasculorum (Thomson et al., 1984) Gardan et al., 2003, Xanthomonas campestris pv. beticola (Pammel) Dowson, and Pseudomonas syringae pv. aptata (Brown and Jamieson) Young, Dye, and Wilkie are bacterial pathogens of beetroot (Beta vulgaris L.) and a concern for its sustainable cultivation. Effects of titanium dioxide (TiO₂) and zinc oxide (ZnO) NPs at 0.25 and 0.50 mL∙L⁻¹ were determined on P. betavasculorum, X. campestris pv. beticola, and P. syringae pv. aptata, and on growth, chlorophyll and carotenoid contents, superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), phenylalanine ammonia-lyase (PAL), glutathione (GSH), proline, H₂O₂, and malondialdehyde (MDA) contents of beetroot. Inoculation of beetroot with P. betavasculorum, X. campestris pv. beticola, and P. syringae pv. aptata reduced plant growth, and chlorophyll and carotenoid contents, but increased SOD, CAT, APX, PAL, GSH, proline, H₂O₂, and MDA. Application of TiO₂ or ZnO NPs to plants with, or without, bacteria increased plant growth, chlorophyll, carotenoid, SOD, CAT, APX, PAL, GSH, proline and H₂O₂ contents, but decreased MDA content. Use of ZnO NPs was better than TiO₂ NPs in increasing plant growth, chlorophyll and carotenoid, SOD, CAT, APX, PAL, GSH, proline and H₂O₂ contents. The higher concentrations of both NPs were better in increasing plant growth, chlorophyll and carotenoid, SOD, CAT, APX, PAL, GSH, proline and H₂O₂ contents, and decreased MDA than the lower NP concentration. Soft rot and bacterial pocket indices caused by P. betavasculorum and X. campestris pv. beticola were 4 of 5; leaf spot index caused by P. syringae pv. aptata was 3 of 5. Disease indices were reduced to 1 of 3/4 when 0.50 mL∙L⁻¹ ZnO NPs was applied to plants challenged with bacterial pathogens. The ZnO NPs produced greater increase in plant growth and reduced disease indices than did TiO₂ NP, and may be used for management of bacterial diseases of beetroot.