Vegetation is a crucial and intuitive index that can be used to evaluate the ecological status. Since the 20th century, land use has changed significantly in Yellow River Basin (YRB), along with great changes of vegetation, serious soil erosion, and gradual ecological deterioration. To improve the ecological environment in the YRB, China has carried out a series of ecological protection projects since the 1970s. Therefore, long-term sequence monitoring of vegetation in YRB is necessary to show the conservation effect and better support the further protection and restoration. This study analysed vegetation changes from 2000 to 2019 based on an annual mean fractional vegetation cover (FVC) dataset. The Theil–Sen median trend analysis method was used to analyse trends in FVC. The results showed that the vegetation in the YRB has improved significantly, with an average annual growth rate of 0.65%, and the ‘green line’ of vegetation has moved approximately 300 km westward. The influence of climate on vegetation is essential; therefore, this study also analysed the influence of temperature and precipitation on vegetation over time and space. Ecological control and afforestation are important anthropogenic factors that affect vegetation. The growth trend (0.6%/a) in key ecological function regions (KEFRs) was the fastest, and even though the protection measures are not strict, they provide space for afforestation. The China Ecological Conservation Red Line (CECRL) and the national nature reserves (NNRs) showed relatively flat trends. Ecological afforestation projects were closely correlated with the growth trend of the FVC. The correlation between FVC and the intensity of ecological engineering was significant in typical areas.

An explicit analysis of the impact for the richness of species of the vegetation phenological characteristics calculated from various remote sensing data is critical and essential for biodiversity conversion and restoration. This study collected long-term the Normalized Difference Vegetation Index (NDVI), the Leaf Area Index (LAI), the Fraction of Absorbed Photosynthetically Active Radiation (FAPAR), and the Fractional Vegetation Cover (FVC), and calculated the six vegetation phenological characteristic parameters: the mean of the growing season, the mean of the mature season, the mean of the withered season, the annual difference value, the annual cumulative value, and the annual standard deviation in the Xinjiang Uygur Autonomous Region. The relationships between the vegetation phenological characteristics and the species richness of birds and mammals were analyzed in spatial distribution. The main findings include: (1) The correlation between bird diversity and vegetation factors is greater than that of mammals. (2) For remote sensing data, FAPAR is the most important vegetation parameter for both birds and mammals. (3) For vegetation phenological characteristics, the annual cumulative value of the LAI is the most crucial vegetation phenological parameter for influencing bird diversity distribution, and the annual difference value of the NDVI is the most significant driving factor for mammal diversity distribution.