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Prof. Hafiz M. N. Iqbal
Tecnologico de Monterrey, School of Engineering and Sciences, Campus Monterrey, Mexico.

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0 Biocatalysis
0 Biomaterials
0 Bioremediation
0 Biosensors
0 Enzymes

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Enzymes
pollutants
immobilization
Biocatalysis
Bioremediation
Biomaterials
biomolecules
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Project

Project Goal: Call for papers

Starting Date:15 May 2020

Current Stage: Active @ https://www.researchgate.net/project/Call-for-papers-for-CSCEE-Special-Issue-Environmental-engineering

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Project

Project Goal: Call for papers

Starting Date:04 March 2020

Current Stage: Active @ https://www.researchgate.net/project/Special-Issue-Nano-Bio-catalysis-Fundamental-Aspects-and-Potential-Applications

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Project

Project Goal: Call for papers

Starting Date:03 March 2020

Current Stage: Active @ https://www.researchgate.net/project/Special-Issue-Advances-and-New-Perspectives-in-Marine-Biotechnology-20

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Journal article
Published: 13 August 2021 in Science of The Total Environment
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The growing persistence of estrogenic pollutants in water resources is a worrying concern because of their endocrine disrupting activities and potentially hazardous consequences on the environmental matrices, ecology, and human health, even at low concentration. The long-term persistence of steroidal estrogens leads to their bioaccumulation in aquatic organisms that can further reach to humans via food chain route. Considering the toxicity of steroidal estrogens, it is important to mitigate these environmentally related hazardous contaminants. So far, several treatment methods, like adsorption, oxidation, irradiation, and electrochemical techniques have been proposed to eliminate estrogens from aqueous ecosystems. Nevertheless, high operational costs, insufficient removal, generation of toxic sludge, and the necessity of skilled maintenance and operating workers are the major hindrances associated with large scale applications. Bioremediation of steroidal estrogens using enzyme-based biocatalytic system has recently emerged as a promising alternative to remove and bio-transform estrogens from aqueous systems. However, the current literature lacks a critique focusing specifically and comprehensively on steroidal estrogens. The presented review is a critical assessment of the existing literature on steroid-based endocrine disruptive estrogens. A detailed description about the occurrence and eco-fate of steroidal estrogens is given with representative examples. The later half of the review stresses on the redefining (removal) of endocrine disruptive estrogens in water resources with particular reference to enzyme-based approaches.

ACS Style

Muhammad Bilal; Damià Barceló; Hafiz M.N. Iqbal. Occurrence, environmental fate, ecological issues, and redefining of endocrine disruptive estrogens in water resources. Science of The Total Environment 2021, 800, 149635 .

AMA Style

Muhammad Bilal, Damià Barceló, Hafiz M.N. Iqbal. Occurrence, environmental fate, ecological issues, and redefining of endocrine disruptive estrogens in water resources. Science of The Total Environment. 2021; 800 ():149635.

Chicago/Turabian Style

Muhammad Bilal; Damià Barceló; Hafiz M.N. Iqbal. 2021. "Occurrence, environmental fate, ecological issues, and redefining of endocrine disruptive estrogens in water resources." Science of The Total Environment 800, no. : 149635.

Review article
Published: 29 July 2021 in Chemosphere
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Covalent organic frameworks (COFs) are an emergent group of crystalline porous materials that have gained incredible interest in recent years. With foreseeable controllable functionalities and structural configurations, the constructions and catalytic properties of these organic polymeric materials can be controlled to fabricate targeted materials. The specified monomer linkers and pre-designed architecture of COFs facilitate the post-synthetic modifications for introducing novel functions and useful properties. By virtue of inherent porosity, robust framework, well-ordered geometry, functionality, higher stability, and amenability to functionalization, COFs and COFs-based composites are regarded as prospective nanomaterials for environmental clean-up and remediation. This report spotlights the state-of-the-art advances and progress in COFs-based materials to efficiently mitigate pharmaceutical-based environmental pollutants from aqueous solutions. Synthesis approaches, structure, functionalization, and sustainability aspects of COFs are discussed. Moreover, the adsorptive and photocatalytic potential of COFs and their derived nanocomposites for removal and degradation of pharmaceuticals are thoroughly vetted. In addition to deciphering adsorption mechanism/isotherms, the stability, regeneratability and reproducibility are also delineated. Lastly, the outcomes are summed up, and new directions are proposed to widen the promise of COF-based smart materials in diverse fields.

ACS Style

Jiansong Gan; Xiaobing Li; Komal Rizwan; Muhammad Adeel; Muhammad Bilal; Tahir Rasheed; Hafiz M.N. Iqbal. Covalent organic frameworks-based smart materials for mitigation of pharmaceutical pollutants from aqueous solution. Chemosphere 2021, 286, 131710 .

AMA Style

Jiansong Gan, Xiaobing Li, Komal Rizwan, Muhammad Adeel, Muhammad Bilal, Tahir Rasheed, Hafiz M.N. Iqbal. Covalent organic frameworks-based smart materials for mitigation of pharmaceutical pollutants from aqueous solution. Chemosphere. 2021; 286 ():131710.

Chicago/Turabian Style

Jiansong Gan; Xiaobing Li; Komal Rizwan; Muhammad Adeel; Muhammad Bilal; Tahir Rasheed; Hafiz M.N. Iqbal. 2021. "Covalent organic frameworks-based smart materials for mitigation of pharmaceutical pollutants from aqueous solution." Chemosphere 286, no. : 131710.

Original paper
Published: 27 July 2021 in Clean Technologies and Environmental Policy
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This work reports the fabrication and catalytic exploitation of in-house engineered green composite (CaO/W-Mo) having catalytic potentialities with the aid of a wet-impregnation route. The in-house engineered CaO/W-Mo hybrid composite was fully characterized using FT-IR (Fourier transform-infrared spectroscopy), PXRD (powder X-ray diffraction), and SEM (scanning electron microscopy). The low concentration, i.e., 0.1–0.5% of hybrid composite (CaO/W-Mo), successfully converts the Brassica nigra oil into biodiesel through one-step transesterification reaction. The catalytic conversion was confirmed by FT-IR, 1H NMR (1H-nuclear magnetic resonance), and GC–MS (gas chromatography–mass spectrometry) analyses. Various fuel properties were determined using the ASTM (American Society for Testing and Materials) method. The optimization of transesterification efficiency was investigated by changing the different factors, such as catalyst concentration, oil to methanol ratio, and reaction period. The experimental results revealed that the highly economical catalyst loading (0.5%) gives transesterification efficiency up to 50% under optimized reaction conditions with an oil-to-methanol ratio 1:12 and reaction temperature 60 °C. This shows that the hybrid composite as a catalyst at minimum concentration could be highly beneficial, economical, and eco-friendly for the industrial-scale production of bioenergy.

ACS Style

Ushna Fatima; Farooq Ahmad; Muhammad Ramzan; Saba Aziz; Muhammad Tariq; Hafiz M. N. Iqbal; Muhammad Imran. Catalytic transformation of Brassica nigra oil into biodiesel using in-house engineered green catalyst: Development and characterization. Clean Technologies and Environmental Policy 2021, 1 -11.

AMA Style

Ushna Fatima, Farooq Ahmad, Muhammad Ramzan, Saba Aziz, Muhammad Tariq, Hafiz M. N. Iqbal, Muhammad Imran. Catalytic transformation of Brassica nigra oil into biodiesel using in-house engineered green catalyst: Development and characterization. Clean Technologies and Environmental Policy. 2021; ():1-11.

Chicago/Turabian Style

Ushna Fatima; Farooq Ahmad; Muhammad Ramzan; Saba Aziz; Muhammad Tariq; Hafiz M. N. Iqbal; Muhammad Imran. 2021. "Catalytic transformation of Brassica nigra oil into biodiesel using in-house engineered green catalyst: Development and characterization." Clean Technologies and Environmental Policy , no. : 1-11.

Article
Published: 26 July 2021 in Catalysis Letters
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Phytase, a key biocatalyst with multipurpose applied potentialities, and its significant relationship to contemporary industrial apprehensions provide unique physicochemical and catalytic attributes. Owing to the rising research advancement, the convergence of technologies fosters additional merits to this industrially relevant biocatalyst, i.e., phytase. Enriched with multipurpose characteristics, phytase holds a significant global market share expected to reach up to $590 million USD by 2024 compared to its share from $380 million USD in 2019. Major applications of phytase are in the pharmaceutical, food, and feed sectors. Growth performance, nutrient digestibility, and mineral availability are vital factors in aquaculture. The manufacturing cost of feed in aquaculture is about 50–80%. Fish meal is a rich protein source; however, plant-based proteins are preferred due to high price issues. Plant-based diets contain antinutritional factors, primarily phytate, and fishes are incapable of metabolizing phytate. About 1% phosphorus (P) is present in the fish meal, which if not digested by fish and remain undigested, causing water pollution. Phytate has unfavorable impacts on fish growth and body composition. Phytate binds with phosphorus in fishes to make phytate-phosphorus complex and make it unavailable for fishes. Phytate also forms complexes commonly with cations such as calcium, iron, copper, magnesium, and so on, limiting the bioavailability of minerals. Phytate forms a bond with trypsin and intervenes with available protein. Phytase enzyme emancipates inorganic phosphorus from phytate when supplemented with plant-based diets. Numerous varieties of stable phytases, handling ease, and resistant to high temperature are discussed herein. The maximum use of phytase in aquaculture nutrition extends to the necessities of economical feed and safety environment.

ACS Style

Syed Makhdoom Hussain; Saba Hanif; Aqsa Sharif; Fatima Bashir; Hafiz M. N. Iqbal. Unrevealing the Sources and Catalytic Functions of Phytase with Multipurpose Characteristics. Catalysis Letters 2021, 1 -14.

AMA Style

Syed Makhdoom Hussain, Saba Hanif, Aqsa Sharif, Fatima Bashir, Hafiz M. N. Iqbal. Unrevealing the Sources and Catalytic Functions of Phytase with Multipurpose Characteristics. Catalysis Letters. 2021; ():1-14.

Chicago/Turabian Style

Syed Makhdoom Hussain; Saba Hanif; Aqsa Sharif; Fatima Bashir; Hafiz M. N. Iqbal. 2021. "Unrevealing the Sources and Catalytic Functions of Phytase with Multipurpose Characteristics." Catalysis Letters , no. : 1-14.

Review article
Published: 14 July 2021 in International Journal of Biological Macromolecules
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Co-immobilization of multi-enzymes has emerged as a promising concept to design and signify bio-catalysis engineering. Undoubtedly, the existence and importance of basic immobilization methods such as encapsulation, covalent binding, cross-linking, or even simple adsorption cannot be ignored as they are the core of advanced co-immobilization strategies. Different strategies have been developed and deployed to green the twenty-first century bio-catalysis. Moreover, co-immobilization of multi-enzymes has successfully resolved the limitations of individual enzyme loaded constructs. With an added value of this advanced bio-catalysis engineering platform, designing, and fabricating co-immobilized enzymes loaded nanostructure carriers to perform a particular set of reactions with high catalytic turnover is of supreme interest. Herein, we spotlight the emergence of co-immobilization strategies by bringing multi-enzymes together with various types of nanocarriers to expand the bio-catalysis scope. Following a brief introduction, the first part of the review focuses on multienzyme co-immobilization strategies, i.e., random co-immobilization, compartmentalization, and positional co-immobilization. The second part comprehensively covers four major categories of nanocarriers, i.e., carbon based nanocarriers, polymer based nanocarriers, silica-based nanocarriers, and metal-based nanocarriers along with their particular examples. In each section, several critical factors that can affect the performance and successful deployment of co-immobilization of enzymes are given in this work.

ACS Style

Muhammad Bilal; Nazim Hussain; Juliana Heloisa Pinê Américo-Pinheiro; Yaaser Q. Almulaiky; Hafiz M.N. Iqbal. Multi-enzyme co-immobilized nano-assemblies: Bringing enzymes together for expanding bio-catalysis scope to meet biotechnological challenges. International Journal of Biological Macromolecules 2021, 186, 735 -749.

AMA Style

Muhammad Bilal, Nazim Hussain, Juliana Heloisa Pinê Américo-Pinheiro, Yaaser Q. Almulaiky, Hafiz M.N. Iqbal. Multi-enzyme co-immobilized nano-assemblies: Bringing enzymes together for expanding bio-catalysis scope to meet biotechnological challenges. International Journal of Biological Macromolecules. 2021; 186 ():735-749.

Chicago/Turabian Style

Muhammad Bilal; Nazim Hussain; Juliana Heloisa Pinê Américo-Pinheiro; Yaaser Q. Almulaiky; Hafiz M.N. Iqbal. 2021. "Multi-enzyme co-immobilized nano-assemblies: Bringing enzymes together for expanding bio-catalysis scope to meet biotechnological challenges." International Journal of Biological Macromolecules 186, no. : 735-749.

Journal article
Published: 13 July 2021 in Plants
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The therapeutic potential of whitish glaucous sub-shrub Haloxylon griffithii (H. griffithii), abundantly present in southern regions of South Asia, has been neglected. The current study aimed to assess the phytochemicals and pharmacological potential of native and gemm forms of H. griffithii. Results of antimicrobial activity revealed that all tested bacteria were susceptible at concentrations ≤50 µg/mL, while tested fungal species were susceptible at ≤25 µg/mL. The values of minimum bactericidal concentrations (MBCs) ranged between 10.75 ± 0.20 to 44.25 ± 0.42 µg/mL, 8.25 ± 0.02 to 28.20 ± 0.80 µg/mL. The value of minimum inhibitory concentration (MIC) of all microbial species was ≤100 µg/mL and the antibiotic mechanism showed that both extracts were highly bactericidal and fungicidal. Results of average log reduction of viable cell count in time kill assay indicated that Pseudomonas aeruginosa (P. aeruginosa) NCTC 1662, Candida albicans (C. albicans) IBL-01, Candidakrusei (C. krusei) ATCC 6258, and Aspergillus flavus (A. flavus) QC 6158 were the most susceptible microbial species. High performance liquid chromatography (HPLC)-based quantification confirmed the presence of gallic acid p.coumeric acid catechin, vanillin, ellagic acid, and salicylic acid, while in native extract only gallic acid. Native and gemm extracts exhibited excellent radical scavenging potential measured by 1,1-diphenyl-2-picryl-hydrazyl radical scavenging assay. Significant thrombolytic activity was found in both extracts with negligible haemolytic activity. Highest percent (%) clot lysis was observed with gemm extracts (87.9 ± 0.85% clot lysis). In summary, we infer that valuable evidence congregated can be exploited for better understanding of gemm H. griffithii’s health benefits, further, to increase its utility with enriching dietary sources of health-promoting compounds.

ACS Style

Shagufta Kamal; Ismat Bibi; Kanwal Rehman; Ameer Zahoor; Amna Kamal; Fatima Aslam; Fatmah Alasmary; Tahani Almutairi; Hassna Alhajri; Siham Alissa; Hafiz Iqbal. Biological Activities of In-House Developed Haloxylon griffithii Plant Extract Formulations. Plants 2021, 10, 1427 .

AMA Style

Shagufta Kamal, Ismat Bibi, Kanwal Rehman, Ameer Zahoor, Amna Kamal, Fatima Aslam, Fatmah Alasmary, Tahani Almutairi, Hassna Alhajri, Siham Alissa, Hafiz Iqbal. Biological Activities of In-House Developed Haloxylon griffithii Plant Extract Formulations. Plants. 2021; 10 (7):1427.

Chicago/Turabian Style

Shagufta Kamal; Ismat Bibi; Kanwal Rehman; Ameer Zahoor; Amna Kamal; Fatima Aslam; Fatmah Alasmary; Tahani Almutairi; Hassna Alhajri; Siham Alissa; Hafiz Iqbal. 2021. "Biological Activities of In-House Developed Haloxylon griffithii Plant Extract Formulations." Plants 10, no. 7: 1427.

Article
Published: 09 July 2021 in Water, Air, & Soil Pollution
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Nitrogen (N) is deficient in more than 90% of soils of Pakistan, mainly because of low organic matter contents. The use of nitrogenous fertilizers is a common practice for sustainable and profitable crop yields. A significant portion of added fertilizers is lost through volatilization, leaching, and denitrification. The low use efficiency of these fertilizers in our climate is a serious concern because of high costs and environmental issues. The present study evaluated the novel synergistic effect of urease and nitrification inhibitors such as ammonium thiosulfate (ATS) and 2-Chloro-6-(trichloromethyl)pyridine (nitrapyrin) to reduce the urea hydrolysis in the soil of three cities of Pakistan, i.e., Faisalabad, Gujranwala, and Sheikhupura, to manage the ammonia as well as N loss. Three different combinations, such as A1, A2, and A3 of both inhibitors, were prepared with varying ratios of 1:1, 0.25:0.75, and 0.75:0.25, respectively. Results showed that the minimum urea hydrolysis of about 2.41, 2.79, and 4.68 IU/g soil was observed with A1 combination after 4th-day observation with the rate of 0.50% concentration for Faisalabad, Gujranwala, and Sheikhupura, respectively. In addition, results showed better urease activity at a pH value of 6.50, incubation time of 30 min, and temperature of 37 °C for all A1, A2, and A3 combinations with 0.50% concentration. Moreover, inhibitor-treated urea showed the maximum plant height of 111, 101, and 101 cm, and root length of 15, 11, and 5 cm, number of tillers of 14, 16, and 19 per panicle, and number of spikes of 37, 21, and 38 per panicle with A1, A2, and A3 combination at 0.50% dose respectively in Faisalabad soil. Overall, it is concluded that 0.50% inhibitor concentration showed the much impressive urease inhibition results followed by 0.25 and 0.10%. However, the application of inhibitors was a good practice to reduce the N loss from soil.

ACS Style

Asim Hussain; Nazish Jahan; Zara Jabeen; Khalil Ur Rehman; Hamza Rafeeq; Muhammad Bilal; Hafiz M. N. Iqbal. Synergistic Effect of Urease and Nitrification Inhibitors in the Reduction of Ammonia Volatilization. Water, Air, & Soil Pollution 2021, 232, 1 -17.

AMA Style

Asim Hussain, Nazish Jahan, Zara Jabeen, Khalil Ur Rehman, Hamza Rafeeq, Muhammad Bilal, Hafiz M. N. Iqbal. Synergistic Effect of Urease and Nitrification Inhibitors in the Reduction of Ammonia Volatilization. Water, Air, & Soil Pollution. 2021; 232 (7):1-17.

Chicago/Turabian Style

Asim Hussain; Nazish Jahan; Zara Jabeen; Khalil Ur Rehman; Hamza Rafeeq; Muhammad Bilal; Hafiz M. N. Iqbal. 2021. "Synergistic Effect of Urease and Nitrification Inhibitors in the Reduction of Ammonia Volatilization." Water, Air, & Soil Pollution 232, no. 7: 1-17.

Review
Published: 06 July 2021 in Molecular Neurobiology
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Traumatic lesions in nerves present high incidence and may culminate in sensorimotor and/or autonomic dysfunctions or a total loss of function, affecting the patient's quality of life. Although the microenvironment favors peripheral nerve regeneration, the regenerative process is not always successful. Some herbs, natural products, and synthetic drugs have been studied as potential pro-regenerative interventions. We reviewed and discussed the most recent articles published over the last ten years in high impact factor journals. Even though most of the articles contemplated in this review were in vitro and animal model studies, those with herbs showed promising results. Most of them presented antioxidant and anti-inflammatory effects. Drugs of several pharmacological classes also showed optimistic outcomes in nerve functional recovery, including clinical trials. The results are hopeful; however, mechanisms of action need to be elucidated, and there is a need for more high-quality clinical studies. The study presents careful compilation of findings of dozens of compounds with consistent pro-regenerative evidence published in respected scientific journals. It may be valuable for health professionals and researchers in the field.

ACS Style

Natália Melo Souza; Mateus Figueiredo Gonçalves; Luiz Fernando Romanholo Ferreira; Muhammad Bilal; Hafiz M. N. Iqbal; Renato Nery Soriano. Revisiting the Role of Biologically Active Natural and Synthetic Compounds as an Intervention to Treat Injured Nerves. Molecular Neurobiology 2021, 1 .

AMA Style

Natália Melo Souza, Mateus Figueiredo Gonçalves, Luiz Fernando Romanholo Ferreira, Muhammad Bilal, Hafiz M. N. Iqbal, Renato Nery Soriano. Revisiting the Role of Biologically Active Natural and Synthetic Compounds as an Intervention to Treat Injured Nerves. Molecular Neurobiology. 2021; ():1.

Chicago/Turabian Style

Natália Melo Souza; Mateus Figueiredo Gonçalves; Luiz Fernando Romanholo Ferreira; Muhammad Bilal; Hafiz M. N. Iqbal; Renato Nery Soriano. 2021. "Revisiting the Role of Biologically Active Natural and Synthetic Compounds as an Intervention to Treat Injured Nerves." Molecular Neurobiology , no. : 1.

Review
Published: 05 July 2021 in 3 Biotech
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Enzyme immobilization is a widely used technology for creating more stable, active, and reusable biocatalysts. The immobilization process also improves the enzyme's operating efficiency in industrial applications. Various support matrices have been designed and developed to enhance the biocatalytic efficiency of immobilized enzymes. Given their unique physicochemical attributes, including substantial surface area, rigidity, semi-conductivity, high enzyme loading, hyper catalytic activity, and size-assisted optical properties, nanomaterials have emerged as fascinating matrices for enzyme immobilization. Tyrosinase is a copper-containing monooxygenase that catalyzes the o-hydroxylation of monophenols to catechols and o-quinones. This enzyme possesses a wide range of uses in the medical, biotechnological, and food sectors. This article summarizes an array of nanostructured materials as carrier matrices for tyrosinase immobilization. Following a detailed background overview, various nanomaterials, as immobilization support matrices, including carbon nanotubes (CNTs), carbon dots (CDs), carbon black (CB), nanofibers, Graphene nanocomposite, platinum nanoparticles, nano-sized magnetic particles, lignin nanoparticles, layered double hydroxide (LDH) nanomaterials, gold nanoparticles (AuNPs), and zinc oxide nanoparticles have been discussed. Next, applied perspectives have been spotlights with particular reference to environmental pollutant sensing, phenolic compounds detection, pharmaceutical, and food industry (e.g., cereal processing, dairy processing, and meat processing), along with other miscellaneous applications.

ACS Style

Asim Hussain; Hamza Rafeeq; Muhammad Qasim; Zara Jabeen; Muhammad Bilal; Marcelo Franco; Hafiz M. N. Iqbal. Engineered tyrosinases with broadened bio-catalysis scope: immobilization using nanocarriers and applications. 3 Biotech 2021, 11, 365 .

AMA Style

Asim Hussain, Hamza Rafeeq, Muhammad Qasim, Zara Jabeen, Muhammad Bilal, Marcelo Franco, Hafiz M. N. Iqbal. Engineered tyrosinases with broadened bio-catalysis scope: immobilization using nanocarriers and applications. 3 Biotech. 2021; 11 (8):365.

Chicago/Turabian Style

Asim Hussain; Hamza Rafeeq; Muhammad Qasim; Zara Jabeen; Muhammad Bilal; Marcelo Franco; Hafiz M. N. Iqbal. 2021. "Engineered tyrosinases with broadened bio-catalysis scope: immobilization using nanocarriers and applications." 3 Biotech 11, no. 8: 365.

Journal article
Published: 28 June 2021 in Chemosphere
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The current environmental research has fascinated the sustainable exploitation of mix bacterial consortium to biodegrade the environmentally-related toxic compounds, including hazardous synthetic dyes. Based on the existing literature evidence, textile and other industrial waste effluents pollute the natural water bodies. Textile effluent contains synthetic dyes which are liberated in the environment without proper treatment. The presence of toxic dyes added to the textile effluents undoubtedly affects the flora and fauna as that untreated water is used for irrigation by local farmers. Many conventional and biological methods are in action for the treatment of wastewater. Physical and chemical processes are expensive as compared to microbial treatments. The use of microbial consortia generates efficient results. Wastewater is a valuable resource, however, up to 80% of wastewater is released to different water matrices. This discernment needs to change for a better tomorrow. In this context, herein, we present a robust microbial-assisted treatment and simultaneously reuse of the treated wastewater as an added value to induce plant growth. Thus, the microbial approach for textile waste treatment release by-product after degradation should be non-toxic for the environment. In the present study, the toxicity of synthetic textile dye named Reactive Red 120, Reactive Orange 122, Reactive Yellow 160, and Reactive Blue 19 was investigated using a bioassay method with plant species namely Sorghum bicolor. Plate and Pot experiment was conducted with respect to untreated Azo dyes, degraded metabolites obtained from single bacteria, and consortium. Efficient Seed germination (89%), shoot length (12.4 cm), root length (15.6 cm) of the plants were observed for bacterial consortium degraded metabolites exposed seeds after comparing with the control. The degraded metabolite also increases protein (45.56 mg/g) and sugar (3.15 mg/g) contents. Bioremediation of various textile industrial effluents saves the ecosystem from the harmful effects of hazardous dyes. The biological decolorization of the textile azo dyes was investigated under co-metabolic conditions. The degraded metabolites can be used to enhance crop productivity and for commercial application. This mandates the current and future research to develop economically feasible and environmentally sustainable wastewater treatment practices.

ACS Style

Navneet Joshi; Uma Bhardwaj; Shellina Khan; Hafiz M.N. Iqbal. Synergistic role of bacterial consortium to biodegrade toxic dyes containing wastewater and its simultaneous reuse as an added value. Chemosphere 2021, 284, 131273 .

AMA Style

Navneet Joshi, Uma Bhardwaj, Shellina Khan, Hafiz M.N. Iqbal. Synergistic role of bacterial consortium to biodegrade toxic dyes containing wastewater and its simultaneous reuse as an added value. Chemosphere. 2021; 284 ():131273.

Chicago/Turabian Style

Navneet Joshi; Uma Bhardwaj; Shellina Khan; Hafiz M.N. Iqbal. 2021. "Synergistic role of bacterial consortium to biodegrade toxic dyes containing wastewater and its simultaneous reuse as an added value." Chemosphere 284, no. : 131273.

Review article
Published: 26 June 2021 in Journal of Drug Delivery Science and Technology
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Characteristics of small group of cancerous cells known as cancer stem cells (CSCs) or tumor initiating cells involve relapse, metastasis, and drug resistant which highly influence the available therapeutic processes. Tumoral drug resistance appears closely related to various acquire or intrinsic characteristics of CSCs e.g., detoxifying enzymes, drug efflux transporters, antiapoptotic protein overexpression, DNA repairability, specific morphology, and quiescence. The specific hypoxic stability and niche impart additional shield against anti-cancer therapeutic approaches for CSCs. Therefore, CSC-based therapeutics are intended to form the core of efficient anticancer strategies. Nanostructured constructs have demonstrated to possess promising applications e.g., targeted drug release, controlled delivery, CSC-targeting drugs, and the development of novel gene-specific diagnostic and treatment modalities. This review describes various drug resistant-related characteristics of CSCs and aim to summarize recent therapeutic and biomedical approaches using nanostructures (nanomedicines). The modification of components presents in extracellular matrix with the nanostructured constructs is considered as one of the promising strategies to upgrade the intra-tumoral drug delivery. Different CSCs-targeting therapies including photothermal therapy (PTT), photodynamic therapy (PDT), hyperthermia, and radiotherapy-based methodologies, which could serve as the novel combination of therapeutic approaches to eliminate CSCs and metastasis have also been described.

ACS Style

Yangyong Mao; Mahpara Qamar; Sarmad Ahmad Qamar; Muhammad Imran Khan; Muhammad Bilal; Hafiz M.N. Iqbal. Insight of nanomedicine strategies for a targeted delivery of nanotherapeutic cues to cope with the resistant types of cancer stem cells. Journal of Drug Delivery Science and Technology 2021, 64, 102681 .

AMA Style

Yangyong Mao, Mahpara Qamar, Sarmad Ahmad Qamar, Muhammad Imran Khan, Muhammad Bilal, Hafiz M.N. Iqbal. Insight of nanomedicine strategies for a targeted delivery of nanotherapeutic cues to cope with the resistant types of cancer stem cells. Journal of Drug Delivery Science and Technology. 2021; 64 ():102681.

Chicago/Turabian Style

Yangyong Mao; Mahpara Qamar; Sarmad Ahmad Qamar; Muhammad Imran Khan; Muhammad Bilal; Hafiz M.N. Iqbal. 2021. "Insight of nanomedicine strategies for a targeted delivery of nanotherapeutic cues to cope with the resistant types of cancer stem cells." Journal of Drug Delivery Science and Technology 64, no. : 102681.

Journal article
Published: 24 June 2021 in Chemosphere
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Over the last few years, industrial and anthropogenic activities have increased the presence of organic pollutants such as dyes, herbicides, pesticides, analgesics, and antibiotics in the water that adversely affect human health and the environment worldwide. Photocatalytic treatment is considered a promising, economical, effective, and sustainable process that utilizes light energy to degrade the pollutants in water. However, certain drawbacks like rapid recombination and low migration capability of photogenerated electrons and holes have restricted the use of photo-catalysts in industries. Hence, despite the abundance of lab-scale research, the technology is still not much commercialized in the mainstream. Several structural modifications in the photo-catalysts have been adopted to enhance the pollutant degradation performance to overcome the same. In this context, the present review article outlines the different advanced heterostructures synthesized to date for improved degradation of three major organic pollutants: antibiotics, dyes, and pesticides. Moreover, the article also emphasizes the degradation kinetics of photo-catalysts and the publication trend in the past decade along with the roadblocks preventing the transfer of technology from the laboratory to industry and new age photo-catalysts for the profitable implications in industrial sectors.

ACS Style

Kavya Bisaria; Surbhi Sinha; Rachana Singh; Hafiz M.N. Iqbal. Recent advances in structural modifications of photo-catalysts for organic pollutants degradation – A comprehensive review. Chemosphere 2021, 284, 131263 .

AMA Style

Kavya Bisaria, Surbhi Sinha, Rachana Singh, Hafiz M.N. Iqbal. Recent advances in structural modifications of photo-catalysts for organic pollutants degradation – A comprehensive review. Chemosphere. 2021; 284 ():131263.

Chicago/Turabian Style

Kavya Bisaria; Surbhi Sinha; Rachana Singh; Hafiz M.N. Iqbal. 2021. "Recent advances in structural modifications of photo-catalysts for organic pollutants degradation – A comprehensive review." Chemosphere 284, no. : 131263.

Journal article
Published: 22 June 2021 in Chemosphere
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MXenes are a rapidly expanding and large family of two-dimensional (2D) materials that have recently garnered incredible research interests for diverse applications domains in various industrial sectors. Owing to unique inherent structural and physicochemical characteristics, such as high surface area, biological compatibility, robust electrochemistry, and high hydrophilicity, MXenes are appraised as a prospective avenue for environmental-clean-up technologies to detect and mitigate an array of recalcitrant hazardous contaminants from environmental matrices. MXene-based nanoarchitectures are thought to mitigate inorganic pollutants via interfacial chemical transformation and sorption, while three different mechanisms, including i) surface complexation and sorption (ii) catalytic activation and removal and (iii) radical's generation-based photocatalytic degradation, are involved in the removal of organic contaminants. Considering the application performance of MXenes on the incessant rise to expansion, in this review, we discuss the wide-spectrum applicability of diverse MXenes-based hybrid nanocomposites in environmental remediation. A brief description related to environmental pollutants, structural properties, chemical abilities, and synthesis route of MXenes is delineated at the start. Afterwards, the adsorption and degradative robustness of MXene-based designer nanomaterials for various contaminants including organic dyes, toxic heavy metals, pesticide residues, phenolics, antibiotics, radionuclides, and many others are thoroughly vetted to prove their potentiality in the arena of wastewater purification and remediation. Lastly, challenges and trends in assessing the wide-range applicability and scalability of MXenes are outlined. Seeing encouraging outcomes in plenty of reports, it can be concluded that MXenes-based nanostructures could be considered the next-generation candidates for water sustainability.

ACS Style

Shuangshuang Zhang; Muhammad Bilal; Muhammad Adeel; Damià Barceló; Hafiz M.N. Iqbal. MXene-based designer nanomaterials and their exploitation to mitigate hazardous pollutants from environmental matrices. Chemosphere 2021, 283, 131293 .

AMA Style

Shuangshuang Zhang, Muhammad Bilal, Muhammad Adeel, Damià Barceló, Hafiz M.N. Iqbal. MXene-based designer nanomaterials and their exploitation to mitigate hazardous pollutants from environmental matrices. Chemosphere. 2021; 283 ():131293.

Chicago/Turabian Style

Shuangshuang Zhang; Muhammad Bilal; Muhammad Adeel; Damià Barceló; Hafiz M.N. Iqbal. 2021. "MXene-based designer nanomaterials and their exploitation to mitigate hazardous pollutants from environmental matrices." Chemosphere 283, no. : 131293.

Review article
Published: 22 June 2021 in Chemosphere
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Effective separation and remediation of environmentally hazardous pollutants are burning areas of research because of a constant increase in environmental pollution problems. An extensive number of emerging contaminants in the environmental matrices result in serious health consequences in animals, humans, and plants, even at trace levels. Therefore, it is of paramount significance to quantify these undesirable pollutants, even at a very low concentration, from the natural environment. Magnetic solid-phase extraction (MSPE) has recently achieved huge attention because of its strong magnetic domain and easy separation through an external magnetic field compared with simple solid-phase extraction. Therefore, MSPE appeared the most promising technique for removing and pre-concentration of emerging pollutants at trace level. Compared to the normal solid-phase extraction, MSPE as magnetic hybrid adsorbents offers the unique advantages of distinct nanomaterials and magnetic hybrid materials. It can exhibit efficient dispersion and rapid recycling when applying to a very complex matrix. This review highlights the possible environmental applications of magnetic hybrid nanoscale materials as effective MSPE sorbents to remediate a diverse range of environmentally toxic pollutants. We believe this study tends to evoke a variety of research thrust that may lead to novel remediation approaches in the forthcoming years.

ACS Style

Nisar Ali; Mahamudul Hassan Riead; Muhammad Bilal; Yong Yang; Adnan Khan; Farman Ali; Shafiul Karim; Cao Zhou; Ye Wenjie; Farooq Sher; Hafiz M.N. Iqbal. Adsorptive remediation of environmental pollutants using magnetic hybrid materials as platform adsorbents. Chemosphere 2021, 284, 131279 .

AMA Style

Nisar Ali, Mahamudul Hassan Riead, Muhammad Bilal, Yong Yang, Adnan Khan, Farman Ali, Shafiul Karim, Cao Zhou, Ye Wenjie, Farooq Sher, Hafiz M.N. Iqbal. Adsorptive remediation of environmental pollutants using magnetic hybrid materials as platform adsorbents. Chemosphere. 2021; 284 ():131279.

Chicago/Turabian Style

Nisar Ali; Mahamudul Hassan Riead; Muhammad Bilal; Yong Yang; Adnan Khan; Farman Ali; Shafiul Karim; Cao Zhou; Ye Wenjie; Farooq Sher; Hafiz M.N. Iqbal. 2021. "Adsorptive remediation of environmental pollutants using magnetic hybrid materials as platform adsorbents." Chemosphere 284, no. : 131279.

Article
Published: 18 June 2021 in Water, Air, & Soil Pollution
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The quality of water resources can be altered by human activities carried out in watersheds. These changes can lead to the occurrence of antibiotic-resistant bacteria and compromise public health. The aim of the present study was to evaluate the presence and concentration of total coliforms and Escherichia coli in the water at the Ecological Park Tietê in São Paulo, the antibiotics resistance of isolated E. coli, and the interaction between season, collection points, and water quality variables. Sample localities were georeferenced and identified as P1—drinking water from the distribution system (23°29′33.46″S, 46°31′16.12″O); P2—main lagoon of the park (23°29′37.59″S, 46°31′28.22″O); and P3—connection between the main lagoon and the Tietê River (23°29′14.66″S, 46°31′26.57″O). Physical–chemical and microbiological variables were measured. Data were subjected to analysis of variance. The isolated effect or the interaction between season, collection points, and variables had the means compared to each other by the Scott-Knott test. The microbiological analysis was performed by inoculating the samples in 3 M™ Petrifilm™ E. coli/Coliform Count Plates (containing agar medium with Violet Red Bile nutrients), incubated at 37 °C for 48 h and the E. coli isolated had their antibiotic resistance profile tested by the disk diffusion technique using Mueller–Hinton agar. Total coliforms and E. coli were not identified at P1. Total coliforms were identified in 64% of the samples and E. coli was identified in 36% of the samples. The microbial contamination of the surface waters of the park presents seasonal variation with higher concentrations of E. coli in the hottest and rainiest seasons (spring and summer). The isolated E. coli showed greater resistance to erythromycin (82%) and amoxicillin (55%) in P2 and to erythromycin (82%) and amoxicillin (27%) in P3, with the presence of multiresistant isolates at both points. No strain showed resistance to amikacin. The high rate of resistance of E. coli to the antibiotics frequently used in human and veterinary medicine demonstrates that the contribution of these substances in aquatic ecosystems over the years has exerted a selection pressure on microorganisms, assisting the appearance and spread of resistant bacteria, changing the environmental biota, and turning these locations in possible reservoirs of antibiotic resistance.

ACS Style

Darlan Storto; Luana Barbosa Carvalho Nara; Dora Inés Kozusny-Andreani; Luiz Sergio Vanzela; Cleber Fernando Menegasso Mansano; Muhammad Bilal; Hafiz M. N. Iqbal; Juliana Heloisa Pinê Américo-Pinheiro. Seasonal Dynamics of Microbial Contamination and Antibiotic Resistance in the Water at the Tietê Ecological Park, Brazil. Water, Air, & Soil Pollution 2021, 232, 1 -18.

AMA Style

Darlan Storto, Luana Barbosa Carvalho Nara, Dora Inés Kozusny-Andreani, Luiz Sergio Vanzela, Cleber Fernando Menegasso Mansano, Muhammad Bilal, Hafiz M. N. Iqbal, Juliana Heloisa Pinê Américo-Pinheiro. Seasonal Dynamics of Microbial Contamination and Antibiotic Resistance in the Water at the Tietê Ecological Park, Brazil. Water, Air, & Soil Pollution. 2021; 232 (7):1-18.

Chicago/Turabian Style

Darlan Storto; Luana Barbosa Carvalho Nara; Dora Inés Kozusny-Andreani; Luiz Sergio Vanzela; Cleber Fernando Menegasso Mansano; Muhammad Bilal; Hafiz M. N. Iqbal; Juliana Heloisa Pinê Américo-Pinheiro. 2021. "Seasonal Dynamics of Microbial Contamination and Antibiotic Resistance in the Water at the Tietê Ecological Park, Brazil." Water, Air, & Soil Pollution 232, no. 7: 1-18.

Micro article
Published: 18 June 2021 in MethodsX
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Nowadays, consumer interest in food with natural ingredients has increased. This need has led to the research of new sources and green extraction methods. Betalains are compounds responsible for giving color to cacti fruits. The aim is to obtain low-sugar betacyanins extracts from jiotilla Escontria chiotilla using aqueous two-phase systems (ATPS) to color food with the extract. The effect of principal parameters of ATPS (Ethyl alcohol- KH2PO4/K2HPO4) as tie-line length (TL;40,50 and 70), phase volume ratios (Vr; 1 and 3) on the partitioning of betacyanins, betaxanthins, total sugars, reducing sugars, and antioxidant activity in the extract was evaluated. The yields were determined from the top and bottom phases of the aforementioned parameters. Multivariate analysis of variance (MANOVA, α = 0.05) showed that TLL and Vr were statistically significant (P < 0.05). The lowest bottom sugar yield (25.78 ± 3.14%) corresponds to TLL = 40, Vr = 3. Under these conditions, the corresponding value for betacyanins yield is 62.98±4.52%. For the first time, the ATPS was used to extract betacyanins from cactus fruit.

ACS Style

Luisaldo Sandate-Flores; José Rodríguez-Rodríguez; Magdalena Rostro-Alanis; Jorge Alejandro Santiago Urbina; Karla Mayolo-Deloisa; Elda M. Melchor-Martínez; Juan Eduardo Sosa-Hernández; Roberto Parra-Saldívar; Hafiz M.N. Iqbal. Validation of aqueous two-phase extraction method. MethodsX 2021, 8, 101421 .

AMA Style

Luisaldo Sandate-Flores, José Rodríguez-Rodríguez, Magdalena Rostro-Alanis, Jorge Alejandro Santiago Urbina, Karla Mayolo-Deloisa, Elda M. Melchor-Martínez, Juan Eduardo Sosa-Hernández, Roberto Parra-Saldívar, Hafiz M.N. Iqbal. Validation of aqueous two-phase extraction method. MethodsX. 2021; 8 ():101421.

Chicago/Turabian Style

Luisaldo Sandate-Flores; José Rodríguez-Rodríguez; Magdalena Rostro-Alanis; Jorge Alejandro Santiago Urbina; Karla Mayolo-Deloisa; Elda M. Melchor-Martínez; Juan Eduardo Sosa-Hernández; Roberto Parra-Saldívar; Hafiz M.N. Iqbal. 2021. "Validation of aqueous two-phase extraction method." MethodsX 8, no. : 101421.

Journal article
Published: 17 June 2021 in Biomolecules
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In the present study, soybean peroxidase (SBP) was covalently immobilized onto two functionalized photocatalytic supports (TiO2 and ZnO) to create novel hybrid biocatalysts (TiO2-SBP and ZnO-SBP). Immobilization caused a slight shift in the pH optima of SBP activity (pH 5.0 to 4.0), whereas the free and TiO2-immobilized SBP showed similar thermal stability profiles. The newly developed hybrid biocatalysts were used for the degradation of 21 emerging pollutants in the presence and absence of 1-hydroxy benzotriazole (HOBT) as a redox mediator. Notably, all the tested pollutants were not equally degraded by the SBP treatment and some of the tested pollutants were either partially degraded or appeared to be recalcitrant to enzymatic degradation. The presence of HOBT enhanced the degradation of the pollutants, while it also inhibited the degradation of some contaminants. Interestingly, TiO2 and ZnO-immobilized SBP displayed better degradation efficiency of a few emerging pollutants than the free enzyme. Furthermore, a combined enzyme-chemical oxidation remediation strategy was employed to degrade two recalcitrant pollutants, which suggest a novel application of these novel hybrid peroxidase-photocatalysts. Lastly, the reusability profile indicated that the TiO2-SBP hybrid biocatalyst retained up to 95% degradation efficiency of a model pollutant (2-mercaptobenzothiazole) after four consecutive degradation cycles.

ACS Style

Rana Morsi; Khadega Al-Maqdi; Muhammad Bilal; Hafiz Iqbal; Abbas Khaleel; Iltaf Shah; Syed Ashraf. Immobilized Soybean Peroxidase Hybrid Biocatalysts for Efficient Degradation of Various Emerging Pollutants. Biomolecules 2021, 11, 904 .

AMA Style

Rana Morsi, Khadega Al-Maqdi, Muhammad Bilal, Hafiz Iqbal, Abbas Khaleel, Iltaf Shah, Syed Ashraf. Immobilized Soybean Peroxidase Hybrid Biocatalysts for Efficient Degradation of Various Emerging Pollutants. Biomolecules. 2021; 11 (6):904.

Chicago/Turabian Style

Rana Morsi; Khadega Al-Maqdi; Muhammad Bilal; Hafiz Iqbal; Abbas Khaleel; Iltaf Shah; Syed Ashraf. 2021. "Immobilized Soybean Peroxidase Hybrid Biocatalysts for Efficient Degradation of Various Emerging Pollutants." Biomolecules 11, no. 6: 904.