Microorganisms in the guts of insects enhance the adaptability of their hosts with different lifestyles, or those that live in different habitats. Tuta absoluta is an invasive pest that is a serious threat to tomato production in China. It has quickly spread and colonized Xinjiang, Yunnan and other provinces and regions. We used Illumina HiSeq next generation sequencing of the 16S rRNA gene to study and analyze the composition and diversity of the gut microbiota of three geographical populations of T. absoluta. At the phylum level, the most common bacteria in T. absoluta across all three geographical populations were Proteobacteria and Firmicutes. An uncultured bacterium in the Enterobacteriaceae was the dominant bacterial genus in the T. absoluta gut microbiotas. There were no significant differences in alpha diversity metrics among the Spanish, Yunnan and Xinjiang populations. The structures of the gut microbiota of the three populations were similar based on PCoA and NMDS results. The results confirmed that the microbial structures of T. absoluta from different regions were similar.

The regulation of reproductive division of labor in eusocial insects is pivotal for the evolution and maintenance of social organization. In Bombus terrestris, dominance hierarchies emerge among orphan workers through repeated agonistic interactions, forming distinct behavioral ranks. To explore the neural basis of this process, we combined behavioral assays with single-nucleus RNA sequencing to profile brain-wide gene expression across α-, β-, and γ-bumblebee workers. Our analyses revealed pronounced transcriptional divergence among Kenyon cells, which exhibited enrichment in synaptic, insulin, and MAPK signaling pathways. Among the neuropeptides examined, Neuroparsin-A was markedly upregulated in the Kenyon cells and glial cells of dominant workers, while its receptor, OR1, showed strong expression within Kenyon populations, suggesting a conserved neuropeptide–receptor axis in social Hymenoptera. Gene regulatory network inference further identified ecdysone-responsive transcription factors, including br, Eip74EF, Hr38, Hr3 and Hr4, as key regulators linked to neural plasticity and behavioral differentiation. Together, our findings uncover a neuroendocrine mechanism in which Neuroparsin-A signaling coordinates brain transcriptional programs associated with dominance hierarchy formation in queenless bumblebee societies, offering new insights into the molecular underpinnings of eusocial behavior.