Storage pests and the food spoilage they cause are problems of great concern. Using essential oil obtained from different plants as an insecticide against these storage pests can be considered an environmentally friendly pest management option. Lantana camara Linn. (family Verbenaceae) is a flowering species, and is also a noxious weed that can proliferate well in nearly all geographical habitats. A biopesticide derived from the essential oil extracted from this plant can offer an effective solution for controlling storage pests. The goal of this study is to extract and analyse the chemical composition of essential oil obtained from L. camara leaves, and assess its effectiveness as a bioactive substance against three storage pests: Tribolium castaneum, Lasioderma serricorne, and Callosobruchus chinensis. The yield of essential oil extracted from L. camara leaves was about 0.24 ± 0.014%. By employing the GC-MS technique, the major phytochemicals contained in L. camara leaf essential oil were identified as caryophyllene (69.96%), isoledene (12%), and ɑ-copaene (4.11%). The essential oil exhibited excellent fumigant toxicity (LC₅₀ of 16.70 mg/L air for T. castaneum, 4.141 mg/L air for L. serricorne and 6.245 mg/L air for C. chinensis at 24 h), contact toxicity (LC₅₀ of 8.93 mg/cm² for T. castaneum, 4.82 mg/cm² for L. serricorne and 6.24 mg/cm² for C. chinensis after 24 h) along with effective repellent activity towards the test insects. In addition, the oil showed no significant phytotoxicity on the germination of paddy seeds. This presents the potential to utilize a weed in developing a biopesticide for effectively managing stored product insects because of its strong bioactivity.

Botanical insecticides are promising pest control agents. This research investigated the novel pesticidal efficacy of Araucaria heterophylla and Commiphora molmol extracts against four ectoparasites through treated envelopes. Seven days post-treatment (PT) with 25 mg/mL of C. molmol and A. heterophylla, complete mortality of the camel tick, Hyalomma dromedarii and cattle tick, Rhipicephalus (Boophilus) annulatus were reached. Against H. dromedarii, the median lethal concentrations (LC_50s) of the methanol extracts were 1.13 and 1.04 mg/mL and those of the hexane extracts were 1.47 and 1.38 mg/mL, respectively. The LC₅₀ values of methanol and hexane extracts against R. annulatus were 1.09 and 1.41 plus 1.55 and 1.08 mg/mL, respectively. Seven days PT with 12.5 mg/mL, extracts completely controlled Haematopinus eurysternus and Hippobosca maculata; LC₅₀ of Ha. eurysternus were 0.56 and 0.62 mg/mL for methanol extracts and 0.55 and 1.00 mg/mL for hexane extracts, respectively, whereas those of Hi. maculata were 0.67 and 0.78 mg/mL for methanol extract and 0.68 and 0.32 mg/mL, respectively, for hexane extracts. C. molmol extracts contained sesquiterpene, fatty acid esters and phenols, whereas those of A. heterophylla possessed monoterpene, sesquiterpene, terpene alcohols, fatty acid, and phenols. Consequently, methanol extracts of C. molmol and A. heterophylla were recommended as ecofriendly pesticides.

Mosquitoes are one of the most lethal animals in the world and transmit many dangerous human pathogens, causing millions of deaths each year. The search for modern and better mosquito control is an endless effort almost all over the world. Phytochemicals are promising biological agents for getting rid of pests that are harmful to human and animal health and crops, they are inexpensive, biodegradable, and have diverse modes of action. The efficacy of acetone and hexane leaf extracts of Acacia nilotica, Eucalyptus camaldulensis, and Salix safsafs was investigated against the 2nd and 4th larvae and pupae of two vectors, Culex pipiens and Aedes aegypti. The results showed the obvious effect of A. nilotica extract on the mortality of mosquito larvae, the reduction of female eggs, and a higher mortality rate in sunlight than in shadow (fluorescein). Data from field trials revealed that A. nilotica extracts had the greatest effect on larval reduction, reaching 89.8% in 24 h and having a 12-day stability. Polyethylene glycol, sesquiterpenes, and fatty acids were the most common compounds found in A. nilotica, E. camaldulensis, and S. safsafs, respectively. The acacia plant had promising larvicidal activity, safe and effective alternative to chemical insecticides.

Effective management of insect disease vectors requires a detailed understanding of their ecology and behavior. In Anopheles gambiae sensu lato (s.l.) (Diptera: Culicidae) mating occurs during swarming, but knowledge of their mating behavior under natural conditions is limited. Mosquitoes mate in flight over specific landmarks, known as swarm markers, at particular locations. Swarms consist of males; the females usually approach the swarm and depart following copulation. The number of mating pairs per swarm is closely associated with swarm size. The shape and height of swarm markers vary and may depend on the environmental conditions at the swarm’s location. Male–male interactions in mosquito swarms with similar levels of attractive flight activity can offer a mating advantage to some individuals. Flight tone is used by mosquitoes to recognize the other sex and choose a desirable mate. Clarifying these and other aspects of mosquito reproductive behavior can facilitate the development of population control measures that target swarming sites. This review describes what is currently known about swarming behavior in Anopheles gambiae s.l., including swarm characteristics; mating within and outside of swarms, insemination in females, and factors affecting and stimulating swarming.

The Red Palm Weevil (Rhynchophorus ferrugineus (Oliv.) (Coleoptera, Dryophthoridae) is a well-known palm tree pest that has caused enormous economic damage all over the globe. Insecticides are still the primary method of controlling this pest at this period. However, field populations of RPW have been shown to be resistant to pesticides. Using Bacillus spp. might be one of the options for controlling R. ferruginous. In this study, 23 species of Bacillus spp. were isolated from the rhizosphere of date palm trees in Al Ahsa Oasis, Saudi Arabia. The isolates were identified using 16S rRNA gene sequencing. R. ferrugineus larvae and adults were tested on sugarcane pieces that were treated with the B. thuringiensis strain PDC-AHSAA1 and B. cereus strains (PDC-AHSAA2, PDC-AHSA3 and PDC-AHSA4). The LC₅₀ values for larvae and adults were quite low when they were compared with those of the other isolated strains. The B. thuringiensis strain PDC-AHSAA1 was more effective against both the larvae and adults. The determined LC₅₀ values for B. thuringiensis ranged from 4.19 × 10⁷–3.78 × 10⁹. After 21 days, the data on larval mortality and body weight were evaluated. The surviving larvae that were treated with the bacterial isolates did not acquire a substantial weight. For the RPW larvae and adults, the mortality and corrected mortality death rates were increased by increasing the concentration of B. thuringiensis. In conclusion, Bacillus-treated diets negatively influenced the growth and development of the RPW. This research reported on the interaction between the RPW and the rhizosphere Bacillus spp. and highlighted the tremendous potential for the development of microbial resource-based control strategies for this pest.

The rice weevil, Sitophilus oryzae (L.), and the red flour beetle, Tribolium castaneum (Herbst), are key stored-product pests in Egypt and worldwide. The extensive use of synthetic insecticides has led to adverse effects on the environment, human health, and pest resistance. As a result, environmentally friendly pest management alternatives are desperately required. The botanical oils of jojoba, Simmondsia chinensis (L.), and rosemary, Rosmarinus officinalis L. plants growing in Egypt were extracted, identified by gas chromatography/mass spectrometry (GC–MS), and evaluated for their insecticidal activity against S. oryzae and T. castaneum. The main constituents identified in BOs were carvyl acetate (20.73%) and retinol (16.75%) for S. chinensis and camphor (15.57%), coumarin (15.19%), verbenone (14.82%), and 1,8-cineole (6.76%) for R. officinalis. The S. chinensis and R. officinalis BOs caused significant contact toxicities against S. oryzae and T. castaneum adults, providing LC₅₀ values of 24.37, 68.47, and 11.58, 141.8 ppm at 3 days after treatment (DAT), respectively. S. chinensis oil exhibited significant fumigation toxicity against both insects; however, it was more effective against S. oryzae (LC₅₀ = 29.52 ppm/L air) than against T. castaneum (LC₅₀ = 113.47 ppm/L air) at 3 DAT. Although the essential oil (EO) of R. officinalis significantly showed fumigation toxicity for S. oryzae (LC₅₀ = 256.1 and 0.028 ppm/L air at 1 and 3 DAT, respectively), it was not effective against T. castaneum. These BOs could be beneficial for establishing IPM programs for suppressing S. oryzae and T. castaneum.