In nitrogen (N) -limited agricultural systems, a high microbial immobilization of applied fertilizer-N can limit its availability to plants. However, there is scarce information on the effect of the form of fertilizer used on the plant–microorganism competition in clay-rich soils under a severe semi-arid climate. In a field study, we investigated the wheat–microorganism competition after the direct application of NH₄¹⁵NO₃ closely to seeds in arable fields in North Kazakhstan, documenting the effect of the use of liquid versus granular fertilizer and mini-tillage versus no-tillage. Our results barely showed any fertilizer-N translocation in the soil. Plants outcompete microorganisms for fertilizer-N during the vegetation period. Microbial-to-plant ¹⁵N ratios revealed a predominant fertilizer-¹⁵N uptake by plants. The strong competition for N was mainly related to the placement of the fertilizer close to the seeds. Moreover, the long time interval between fertilization and sampling enhanced the competition for N, meaning that previously microbially immobilized N became available to plants through the death of microorganisms and their subsequent mineralization. The fertilizer distribution between microorganisms and plants did depend on the form of fertilizer used, owing to the good solubility of granular fertilizer. The smaller fertilizer-N uptake under the no-tilling condition was probably due to the more intense soil compaction, which caused a reduction in plant growth. The application of fertilizer close to the seeds and the small fertilizer translocation during the vegetation period ultimately resulted in a high level of plant N being derived from the fertilizer.

Cropping systems and agronomic practices play a critical role in regulating soil organic matter dynamics and carbon dioxide (CO₂) emissions, which are key components of the global carbon cycle and climate change mitigation. However, the combined effects of tillage practices and seasonal climatic variability on CO₂ fluxes in chernozem soils (chernozems, WRB classification; highly fertile, humus-rich soils typical of steppe regions) of Northern Kazakhstan remain insufficiently understood. The aim of this study was to quantify soil CO₂ emissions under conventional tillage, no-till, and bare fallow systems during spring wheat cultivation on ordinary chernozems. Field experiments were conducted between 2023 and 2025 in the Kostanay Region (Kazakhstan). Soil CO₂ fluxes were measured using a chamber-based method, while soil temperature, moisture, and microbial community structure were monitored simultaneously. The results revealed pronounced seasonal and interannual variability in CO₂ emissions, ranging from 2 to 27 g CO₂·m⁻²·day⁻¹. Conventional tillage resulted in higher peak emissions due to increased soil aeration and accelerated organic matter mineralization, whereas no-till systems exhibited a more stable seasonal pattern and lower temperature sensitivity of soil respiration (Q₁₀ = 2.40 for no-till and 3.25 for conventional tillage). The application of machine learning techniques (Random Forest) significantly improved the prediction accuracy of CO₂ fluxes (R² = 0.67; RMSE = 3.37 g CO₂·m⁻²·day⁻¹) compared to linear models. These findings provide a scientific basis for the development of climate-smart agricultural practices aimed at improving carbon management in semi-arid steppe agroecosystems.