Novel therapies to prevent bacterial infections are of utmost importance in biomedical research due to the emergence of multidrug-resistant strains of bacteria. Herein, we report the preparation, characterization and antibacterial evaluation of sulfonated polystyrene nanoparticles simultaneously releasing two antibacterial species, nitric oxide (NO) and singlet oxygen (O₂(¹Δ_g)), upon irradiation with visible light. The nanoparticles were prepared by simple and scalable processes from nanofiber membranes with an encapsulated NO photodonor and/or ionically entangled tetracationic porphyrin/phthalocyanine photosensitizers. The release of NO and O₂(¹Δ_g) from the polystyrene nanoparticles is controlled by light wavelength and dose, as well as by temperature, which influences the diffusion coefficient and solubility of both species in the polystyrene matrix. The concentrations of NO and O₂(¹Δ_g) were measured by amperometric and time-resolved spectroscopic techniques and by chemical analysis. Due to the efficient photogeneration of both species at physiological temperature and resultant strong antibacterial action observed on Escherichia coli, the nanoparticles are a promising material for antibacterial applications triggered/modulated by light and temperature.

Chemotherapeutics such as platinum-based drugs are commonly used to treat several cancer types, but unfortunately, their use is limited by several side effects, such as high degradation of the drug before entering the cells, off-target organ toxicity and development of drug resistance. An interesting strategy to overcome such limitations is the development of nanocarriers that could enhance cellular accumulation in target cells in addition to decreasing associated drug toxicity in normal cells. Here, we aim to prepare and characterize a graphene-oxide-based 2D nanoplatform functionalised using highly branched, eight-arm polyethylene-glycol, which, owing to its high number of available functional groups, offers considerable loading capacity over its linear modalities and represents a highly potent nanodelivery platform as a versatile system in cancer therapy. The obtained results show that the GO@PEG carrier allows for the use of lower amounts of Pt drug compared to a Pt-free complex while achieving similar effects. The nanoplatform accomplishes very good cellular proliferation inhibition in osteosarcoma, which is strictly related to increased cellular uptake. This enhanced cellular internalization is also observed in glioblastoma, although it is less pronounced due to differences in metabolism compared to osteosarcoma. The proposed GO@PEG nanoplatform is also promising for the inhibition of migration, especially in highly invasive breast carcinoma (i.e., MDA-MB-231 cell line), neutralizing the metastatic process. The GO@PEG nanoplatform thus represents an interesting tool in cancer treatment that can be specifically tailored to target different cancers.

Clinically approved photodynamic therapy (PDT) is a minimally invasive treatment procedure that uses three key components: photosensitization, a light source, and tissue oxygen. However, the photodynamic effect is limited by both the photophysical properties of photosensitizers as well as their low selectivity, leading to damage to adjacent normal tissue and/or inadequate biodistribution. Nanoparticles (NPs) represent a new option for PDT that can overcome most of the limitations of conventional photosensitizers and can also promote photosensitizer accumulation in target cells through enhanced permeation and retention effects. In this in vitro study, the photodynamic effect of TPP photosensitizers embedded in polystyrene nanoparticles was observed on the non-tumor NIH3T3 cell line and HeLa and G361 tumor cell lines. The efficacy was evaluated by viability assay, while reactive oxygen species production, changes in membrane mitochondrial potential, and morphological changes before and after treatment were imaged by atomic force microscopy. The tested nanoparticles with embedded TPP were found to become cytotoxic only after activation by blue light (414 nm) due to the production of reactive oxygen species. The photodynamic effect observed in this evaluation was significantly higher in both tumor lines than the effect observed in the non-tumor line, and the resulting phototoxicity depended on the concentration of photosensitizer and irradiation time.

Interactions between hyaluronic acid (HA) and the CD44 receptor represent a key mechanism in tumor cell recognition and selective drug uptake. In this study, we compare the efficacy of a graphene oxide (GO)-based nanoplatform in two cell lines with markedly different CD44 expression levels. The aim is to investigate how HA functionalization and its concentration influence the biological behavior of these GO nanocarriers designed for targeted delivery of doxorubicin (DOX). The nanoplatform was prepared by sequential PEGylation of nanosized GO, followed by HA conjugation at three concentrations (0.1, 1, and 10 mg/mL) and subsequent DOX loading. Spectroscopic and microscopic analyses confirmed stepwise surface modification, formation of a stable polymer coating, and successful DOX incorporation through π-π stacking and hydrogen bonding. Biological assays demonstrated that HA enhances CD44-mediated internalization and increases anticancer activity in CD44⁺ HT-1080 cells, while the GO@PEG carrier alone showed minimal cytotoxicity, highlighting its good biocompatibility. In contrast, CD44⁻ SKBR3 cells displayed limited uptake and higher viability, consistent with weaker HA-CD44 interactions and lower receptor expression. Confocal microscopy and Raman spectroscopy visualized effective intracellular accumulation and perinuclear localization of the nanocarrier, further confirming selective internalization mechanisms. Overall, the results provide important insight into the role of HA in improving the specificity, cellular uptake, and safety of GO-based nanoplatforms. The study underscores the significance of CD44 receptor levels in determining therapeutic efficiency and supports the development of receptor-targeted, biocompatible nanocarrier systems for precision cancer therapy.