The fungi of the genus Alternaria are among the main pathogens causing post-harvest diseases and significant economic losses. The consumption of Alternaria contaminated foods may be a major risk to human health, as many Alternaria species produce several toxic mycotoxins and secondary metabolites. To protect consumer health and extend the shelf life of food products, the development of new ways of packaging is of outmost importance. The aim of this work was to investigate the antifungal capacity of a biodegradable poly(lactic acid) (PLA) package filled with thymol or carvacrol complexed in β-cyclodextrins (β-CDs) by the solubility method. Once solid complexes were obtained by spray drying, varying proportions (0.0%, 1.5%, 2.5%, and 5.0 wt%) of β-CD–thymol or β-CD–carvacrol were mixed with PLA for packaging development by injection process. The formation of stable complexes between β-CDs and carvacrol or thymol molecules was assessed by Fourier-transform infrared spectroscopy (FTIR). Mechanical, structural, and thermal characterization of the developed packaging was also carried out. The polymer surface showed a decrease in the number of cuts and folds as the amount of encapsulation increased, thereby reducing the stiffness of the packaging. In addition, thermogravimetric analysis (TGA) revealed a slight decrease in the temperature of degradation of PLA package as the concentration of the complexes increased, with β-CD–carvacrol or β-CDs–thymol complexes acting as plasticisers that lowered the intermolecular forces of the polymer chains, thereby improving the breaking point. Packages containing 2.5% and 5% β-CD–carvacrol, or 5% β-CD–thymol showed Alternaria alternata inhibition after 10 days of incubation revealing their potential uses in agrofood industry.

Using cyclodextrins (CDs) in packaging technologies helps volatile or bioactive molecules improve their solubility, to guarantee the homogeneous distribution of the complexed molecules, protecting them from volatilization, oxidation, and temperature fluctuations when they are associated with polymeric matrices. This technology is also suitable for the controlled release of active substances and allows the exploration of their association with biodegradable polymer targeting to reduce the negative environmental impacts of food packaging. Here, we present a fresh look at the current status of and future prospects regarding the different strategies used to associate cyclodextrins and their derivatives with polymeric matrices to fabricate sustainable and biodegradable active food packaging (AFP). Particular attention is paid to the materials and the fabrication technologies available to date. In addition, the use of cutting-edge strategies, including the trend of nanotechnologies in active food packaging, is emphasized. Furthermore, a critical view on the risks to human health and the associated updated legislation is provided. Some of the more representative patents and commercial products that currently use AFP are also listed. Finally, the current and future research challenges which must be addressed are discussed.

Blackberries and raspberries are highly perishable and fragile products, which limits their shelf life. The effect of biodegradable active packaging of blackberries and raspberries containing 2.5% and 5.0% weight (wt%) of thymol or carvacrol complexed in β-cyclodextrins (β-CDs), successively added to poly (lactic acid) (PLA), and melt-processed by injection molding was evaluated under stored conditions at 4 °C for 21 days, using as reference commercial clamshell and PLA package control samples. Thus, physicochemical, headspace, microbiological, and sensory quality studies were carried out in order to compare the efficacy of the different packages. Concerning weight loss, color, and total phenolic and soluble solids content, significant differences were detected when compared with commercial clamshell packaging. The results show that the PLA packages containing thymol and carvacrol complexes maintained the color, weight, and phenolic content of berries until day 21, with a score up to 45% better compared to commercial clamshell. The headspace analysis detected 101 mg L⁻¹ (ppm) of thymol and 35 ppm of carvacrol on the first day of refrigeration; these concentrations decreased with time. This release mechanism of carvacrol and thymol into the PLA package modified the initial atmosphere composition. After 21 days of storage, the berries had 4.25 degrees of acceptance, without adverse perception of aroma or flavor for both carvacrol and thymol compounds. A general microbial inhibition was observed for yeast and molds, which increased with the concentration of monoterpene in PLA packages, and showed an inhibition of 3.5 log units for PLA packages containing thymol, and of 3 log units for those containing carvacrol. Overall results show that PLA/β-CD-thymol 5.0% packages prolonged raspberries’ and blackberries’ shelf life by one more week at 4 °C, compared with commercial clamshell packaging.

Amaranth is one of the most representative crops in Mexico and could represent an alternative for consumers with celiac disease. Since it possesses no gluten, it can be included in the production of non-conventional starchy foods. This work was focused on the evaluation of process conditions and adequacy of a gluten-free lasagna. Four amaranth formulations containing corn and tapioca starches at different concentrations were analyzed. Ready-to-cook samples were obtained after drying (80°C for 45 min). Pasta containing 8% corn starch reported the highest stability during blending (1.5 min) and a firmness value of 16.060 kg. This formulation was subjected to sensory analysis by 10 celiac consumers. Overall acceptability received an average score of 4.7 on a 9-point hedonic scale with a possible intention of buying.

Tenate cheese is a traditional Mexican pressed semi-hard cheese made from raw cow’s milk and wrapped in palm fiber. The characterization of this cheese remains scarce. This study presents an exploratory characterization of a single production batch of traditional Tenate cheese obtained from one artisanal producer, providing preliminary information on its physicochemical, microbiological, and proximate analyses, combined with consumer evaluation. The latter was analyzed using hierarchical cluster analysis (HCA) as an exploratory segmentation tool. Tenate cheese was characterized as a semi-hard cheese with active lactic fermentation, a lactic aroma, acidic and umami flavors, and a firm, granular texture. Microbiological analyses showed the absence of coliforms, enterobacteria, and Staphylococcus aureus among the microorganisms evaluated, whereas yeast counts exceeded the regulatory limit. As major foodborne pathogens were not included in the microbiological assessment, the overall microbiological safety of the product could not be confirmed. A total of 318 consumers from Aguascalientes (AGS, n = 149) and the Guadalajara Metropolitan Area (GMA, n = 169) evaluated the product using hedonic and Just-About-Right scales. Consumers from AGS reported significantly higher liking scores than those from GMA. Penalty analysis identified insufficient softness as the main attribute associated with lower liking in AGS, whereas low flavor intensity and weak aftertaste reduced acceptance in GMA. Hierarchical cluster analysis (HCA) identified three consumer segments in each location, revealing distinct preference patterns linked to regional expectations. The main contributions of this study are threefold. First, it contributes to the limited scientific knowledge available on Tenate cheese by providing a comprehensive characterization of the analyzed sample. Second, it shows that consumer acceptance differed between two regional markets, comparing two university-affiliated consumer groups, highlighting the value of consumer segmentation for product positioning. Third, it proposes and applies an integrated framework combining physicochemical, microbiological, nutritional, sensory, and consumer segmentation analyses that can be applied to the study of other artisanal cheeses.