The main objective of the present study was to apply a slope-based spectral method to both dry and fresh pasture vegetation. Differences in eight spectral ranges were identified across the near infrared-shortwave infrared (NIR-SWIR) that were indicative of changes in chemical properties. Slopes across these ranges were calculated and a partial least squares (PLS) analytical model was constructed for the slopes vs. crude protein (CP) and neutral detergent fiber (NDF) contents. Different datasets with different numbers of fresh/dry samples were constructed to predict CP and NDF contents. When using a mixed-sample dataset with dry-to-fresh ratios of 85%:15% and 75%:25%, the correlations of CP (R² = 0.95, in both) and NDF (R² = 0.84 and 0.82, respectively) were almost as high as when using only dry samples (0.97 and 0.85, respectively). Furthermore, satisfactory correlations were obtained with a dry-to-fresh ratio of 50%:50% for CP (R² = 0.92). The results of our study are especially encouraging because CP and NDF contents could be predicted even though some of the selected spectral regions were directly affected by atmospheric water vapor or water in the plants.

In this study, we develop a spectral method for assessment of pasture quality based only on the spectral information obtained with a small number of wavelengths. First, differences in spectral behavior were identified across the near infrared–shortwave infrared spectral range that were indicative of changes in chemical properties. Then, slopes across different spectral ranges were calculated and correlated with the changes in crude protein (CP), neutral detergent fiber (NDF) and metabolic energy concentration (MEC). Finally, partial least squares (PLS) regression analysis was applied to identify the optimal spectral ranges for accurate assessment of CP, NDF and MEC. Six spectral domains and a set of slope criteria for real-time evaluation of pasture quality were suggested. The evaluation of three level categories (low, medium, high) for these three parameters showed a success rate of: 73%–96% for CP, 72%–87% for NDF and 60%–85% for MEC. Moreover, only one spectral range, 1748–1764 nm, was needed to provide a good estimation of CP, NDF and MEC. Importantly, five of the six selected spectral regions were not affected by water absorbance. With some modifications, this rationale can be applied to further analyses of pasture quality from airborne sensors.

Salinization of irrigated lands in the semi-arid Jezreel Valley, Northern Israel results in soil-structure deterioration and crop damage. We formulated a generic rule for estimating salinity of different vegetation types by studying the relationship between Cl/Na and different spectral slopes in the visible–near infrared–shortwave infrared (VIS–NIR–SWIR) spectral range using both field measurements and satellite imagery (Sentinel-2). For the field study, the slope-based model was integrated with conventional partial least squares (PLS) analyses. Differences in 14 spectral ranges, indicating changes in salinity levels, were identified across the VIS–NIR–SWIR region (350–2500 nm). Next, two different models were run using PLS regression: (i) using spectral slope data across these ranges; and (ii) using preprocessed spectral reflectance. The best model for predicting Cl content was based on continuum removal reflectance (R2 = 0.84). Satisfactory correlations were obtained using the slope-based PLS model (R2 = 0.77 for Cl and R2 = 0.63 for Na). Thus, salinity contents in fresh plants could be estimated, despite masking of some spectral regions by water absorbance. Finally, we estimated the most sensitive spectral channels for monitoring vegetation salinity from a satellite perspective. We evaluated the recently available Sentinel-2 imagery’s ability to distinguish variability in vegetation salinity levels. The best estimate of a Sentinel-2-based vegetation salinity index was generated based on a ratio between calculated slopes: the 490–665 nm and 705–1610 nm. This index was denoted as the Sentinel-2-based vegetation salinity index (SVSI) (band 4 − band 2)/(band 5 + band 11).