Objective: Analysis the chemical constituents from the roots of Ficus hirta. Methods: The Ficus hirta Vahl. were extracted with 75% ethanol. Qualitative analysis of ethanol extracts was carried out by using high performance liquid chromatography-tandem mass spectrometry (LCMS-IT-TOF) and gas chromatography-mass spectrometry (GC-MS). Result: Twenty compounds were identified by LCMS-IT-TOF. Nine compounds were identified by GC-MS. Psoralens and bergapten were identified by LCMS-IT-TOF and GC-MS. Conclusion: LC-MS-TOF and GC-MS have identified 27 compounds, including 9 flavonoids, 6 coumarins, 3 organic acids, 3 organic alcohols, 2 organic esters, 1 terpene, 1 polyphenol, 1 alkaloid and 1 anthraquinone, which laid a foundation for further study of Ficus hirta Vahl. and its compound preparations.

Subcritical fluid extraction technology was used to collect the extract of Ficus hirta, and the volatile oil was obtained by molecular distillation. The chemical composition of the volatile oil was characterized by gas chromatography-mass spectrometry (GC-MS), and the relative content of each component in the volatile oil was obtained by GC-MS peak area normalization analysis. Subcritical extraction technology combined with molecular distillation method is not only can be very good in the preparation of volatile oil Ficus hirta, but also can preserved its rich fragrance; Ficus hirta volatile oil identified a total of 29 kinds of substances, of which the higher levels of components of caproic acid, pelargonic acid, psoralen, caprylic acid. This method is simple and feasible, what's more, it can be used to prepare the composition of the volatile oil from Ficus hirta preliminary identification.