Saffron (Crocus sativus L.) is a valuable geophyte plant and one of the most expensive spices in the world. Recently, the demand for saffron spice has increased in worldwide markets owing to its enormous application and value. However, the production of saffron is limited by the vegetative propagation technique and the limited number of high-quality corms planted. Furthermore, climatic changes, notably increasing temperatures, negatively influence saffron multiplication and growth. Thus, it is important to develop alternative cultivation and propagation techniques for saffron under a controlled environment, which could ensure an increase in saffron yield and avoid the negative impact of climatic changes. The present study aimed to develop an alternative method for vegetative propagation of Crocus sativus under controlled conditions. The effect of different cross-cuttings, including basal cuttings (BCs) and top-to-bottom cuttings (CTBs), was evaluated on shoot, leaf, flower, and daughter corm production. All the growth parameters examined were influenced by the cutting treatment applied. The results showed that the highest number of shoots formed was obtained by BCs and CTBs, with an average of 6.68 and 5.47 shoots per corm, respectively, compared to the control with an average of 2.70 shoots per corm. The cutting treatment positively affected the formation of daughter corms in which, the high mean number of corms recorded was obtained by the BC treatment. Meanwhile, the lower size of the daughter corms was obtained after the cross-cutting treatment. This is the first report that provides an alternative propagation for saffron using a controlled environment, which could help to improve the production of saffron.

The ornamental plant Spathiphyllum wallisii is becoming increasingly popular worldwide, and tissue culture is regarded as a practical option for its massive production. Following the multiplication phase and successive subcultures for an extended period, some morphological changes in shoots were observed in Spathiphyllum explants, primarily a reduction in leaf area. For the first time, we report the application of silver nitrate to enhance Spathiphyllum shoot and root quality. The Murashige and Skoog (MS) medium, with 30 g·L⁻¹ of sucrose and 100 mg·L⁻¹ of ascorbic acid, was supplemented with different concentrations of AgNO₃. The addition of AgNO₃ improved shoot development and leaf expansion by increasing their length and width. Specifically, at the 2.0–4.0 mg·L⁻¹ range, silver nitrate successfully induced the highest leaf area (1.7 cm²), while stem elongation was promoted with the application of 3.0 mg·L⁻¹ of AgNO₃. Additionally, optimal leaf numbers of new shoots were observed at 1 mg·L⁻¹. Furthermore, when used at an optimal concentration, AgNO₃ significantly boosted rhizogenesis, increasing the number (9.2 roots), length (5.8 cm), and diameter (1 mm) of roots per explant. After the plantlets were transferred to pots, they acclimatized successfully with a 100% survival rate. This finding underscores the potential of silver nitrate to optimize the growth and rooting of Spathiphyllum shoots, paving the way for a more efficient micropropagation process.