Temperature and food quality are the most important environmental factors determining the performance of herbivorous insects. The objective of our study was to evaluate the responses of the spongy moth (formerly known as the gypsy moth) [Lymantria dispar L. (Lepidoptera: Erebidae)] to simultaneous variation in these two factors. From hatching to the fourth instar, larvae were exposed to three temperatures (19 °C, 23 °C, and 28 °C) and fed four artificial diets that differed in protein (P) and carbohydrate (C) content. Within each temperature regime, the effects of the nutrient content (P+C) and ratio (P:C) on development duration, larval mass, growth rate, and activities of digestive proteases, carbohydrases, and lipase were examined. It was found that temperature and food quality had a significant effect on the fitness-related traits and digestive physiology of the larvae. The greatest mass and highest growth rate were obtained at 28 °C on a high-protein low-carbohydrate diet. A homeostatic increase in activity was observed for total protease, trypsin, and amylase in response to low substrate levels in the diet. A significant modulation of overall enzyme activities in response to 28 °C was detected only with a low diet quality. A decrease in the nutrient content and P:C ratio only affected the coordination of enzyme activities at 28 °C, as indicated by the significantly altered correlation matrices. Multiple linear regression analysis showed that variation in fitness traits in response to different rearing conditions could be explained by variation in digestion. Our results contribute to the understanding of the role of digestive enzymes in post-ingestive nutrient balancing.

The mealworm Tenebrio molitor L. (Coleoptera: Tenebrionidae) feeds on wheat bran and is considered both a pest and an edible insect. Its larvae contain proteins and essential amino acids, fats, and minerals, making them suitable for animal and human consumption. Zearalenone (ZEA) is the mycotoxin most commonly associated with Fusarium spp. It is found in cereals and cereal products, so their consumption is a major risk for mycotoxin contamination. One of the most important effects of ZEA is the induction of oxidative stress, which leads to physiological and behavioral changes. This study deals with the effects of high doses of ZEA (10 and 20 mg/kg) on survival, molting, growth, weight gain, activity of antioxidant enzymes superoxide dismutase (SOD) and glutathione S-transferase (GST), and locomotion of mealworm larvae. Both doses of ZEA were found to (i) have no effect on survival, (ii) increase molting frequency, SOD, and GST activity, and (iii) decrease body weight and locomotion, with more pronounced changes at 20 mg/kg. These results indicated the susceptibility of T. molitor larvae to high doses of ZEA in feed.

Janković-Tomanić Milena Ž, Doktorska disertacija, Univerzitet u Beogradu, Biološki fakultet, Beograd 2012, Odbranjena 27.12.2012., Jezik srpski - latinica

Magnetic fields are abiotic environmental factors that can cause a wide range of biological effects at both the cellular and whole-organism levels. In this study, we investigated the effects of a static magnetic field (SMF, 110 mT) on life history traits and antioxidant defence mechanisms during the preadult development of Lymantria dispar. SMF exposure did not affect the mass of younger larvae, whereas older larvae and pupae showed significantly reduced mass compared to controls. Estimated larval mortality was higher in the SMF group, while developmental duration was significantly prolonged in the fifth larval instar and in both male and female pupae. SMF induced stage-dependent modifications in antioxidant defence. Superoxide dismutase activity and catalase activities were significantly increased, predominantly in later developmental stages, while glutathione reductase and glutathione S-transferase showed instar-dependent responses. In addition, the content of total and oxidised glutathione was significantly higher in the fifth and sixth instars of SMF-exposed larvae compared to controls. The study shows that static magnetic field exposure can interfere with normal developmental processes and redox homeostasis in insects, implying potential adaptive mechanisms under stressful conditions.