Small ruminants are important hosts for various tick species and tick-associated organisms, many of which are zoonotic. The aim of the present study was to determine the presence of tick-borne protozoans and bacteria of public health and veterinary significance in goats and wild Siberian roe deer (Capreolus pygargus) from Heilongjiang Province, northeastern China. The occurrence of piroplasms, Anaplasma phagocytophilum, A. bovis, A. marginale, A. capra, A. ovis, Ehrlichia spp. and spotted fever group rickettsiae was molecularly investigated and analyzed in 134 goats and 9 free ranging C. pygargus living in close proximity. Piroplasm DNA was detected in 16 (11.9%) goats and 5 C. pygargus. Sequence analysis of 18S rRNA sequences identified 3 Theileria species (T. luwenshuni, T. capreoli and T. cervi). Four Anaplasma species (A. ovis, A. phagocytophilum, A. bovis and A. capra) were identified in goats and C. pygargus. Anaplasma ovis and A. bovis were detected in 11 (8.2%) and 6 (4.5%) goats, respectively; A. phagocytophilum, A. bovis and A. capra were found in 3, 7 and 3 C. pygargus, respectively. Sequence analysis of 16S rRNA sequences revealed the presence of 5 different genetic variants of A. bovis in goats and C. pygargus, while the analysis of 16S rRNA and gltA sequence data showed that A. capra isolates identified from C. pygargus were closely related to the genotype identified from sheep and Haemaphysalis qinghaiensis, but differed with the genotype from humans. Anaplasma/Theileria mixed infection was observed in 2 (1.5%) goats and 5 C. pygargus, and co-existence involving potential zoonotic organisms (A. phagocytophilum and A. capra) was found in 2 C. pygargus. All samples were negative for A. marginale, Ehrlichia spp. and SFG rickettsiae. These findings report the tick-borne pathogens in goats and C. pygargus, and a greater diversity of these pathogens were observed in wild animals. Three Theileria (T. luwenshuni, T. capreoli and T. cervi) and four Anaplasma species (A. ovis, A. phagocytophilum, A. bovis and A. capra) with veterinary and medical significance were identified in small domestic and wild ruminants. The contact between wild and domestic animals may increase the potential risk of spread and transmission of tick-borne diseases.

Amid global health concerns and the constant threat of zoonotic diseases, this study delves into the diversity of circular replicase-encoding single-stranded DNA (CRESS-DNA) viruses within Chinese wild bird populations. Employing viral metagenomics to tackle the challenge of “viral dark matter,” the research collected and analyzed 3,404 cloacal swab specimens across 26 bird families. Metagenomic analysis uncovered a rich viral landscape, with 67.48% of reads classified as viral dark matter, spanning multiple taxonomic levels. Notably, certain viral families exhibited host-specific abundance patterns, with Galliformes displaying the highest diversity. Diversity analysis categorized samples into distinct groups, revealing significant differences in viral community structure, particularly noting higher diversity in terrestrial birds compared to songbirds and unique diversity in migratory birds versus perching birds. The identification of ten novel Circoviridae viruses, seven Smacoviridae viruses, and 167 Genomoviridae viruses, along with 100 unclassified CRESS-DNA viruses, underscores the expansion of knowledge on avian-associated circular DNA viruses. Phylogenetic and structural analyses of Rep proteins offered insights into evolutionary relationships and potential functional variations among CRESS-DNA viruses. In conclusion, this study significantly enhances our understanding of the avian virome, shedding light on the intricate relationships between viral communities and host characteristics in Chinese wild bird populations. The diverse array of CRESS-DNA viruses discovered opens avenues for future research into viral evolution, spread factors, and potential ecosystem impacts.

The outbreaks of African Swine Fever (ASF) in China are ongoing, and the inadequate management of the pig supply chain is criticized. In the past four years, a series of preventive and control measures have been supplied national wide, while the outbreaks haven't been terminated. This suggests the existing animal disease management at the district level may not be appropriate to control ASF under the current situation of the ASF outbreak in China. It is urgent to further describe real distribution areas of ASF in China. In this study, we combined four regional-scale models to predict the risk distribution of ASF in mainland China and identify risk factors related to ASF outbreaks. The results showed that the four regional-scale models were more accurate in predicting the ASF outbreaks than the nationwide scale model. The four regional-scale models identified the potential risk factors associated with ASF outbreaks, such as population density, pig density, land cover, temperature, and elevation factors. Moreover, seven clusters with high potential risk of ASF outbreaks were identified. Then, based on the results, we proposed more suitable prevention and control plans for ASF, which can assist the implementation of transport management policies within and between risk clusters.

Peste-des-Petits Ruminants Virus (PPRV) causes a highly contagious and severe infectious disease known as Peste-des-Petits Ruminants (PPR), resulting in significant mortality in both domestic and wild ruminants. An in‐depth understanding of the molecular relationship between PPRV and susceptible hosts is essential for the prevention of PPR. The signaling lymphocytic‐activation molecule (SLAM) acts as a key receptor in susceptible host species, mediating interactions with PPRV and triggering PPR in ruminants. This study offers an in‐depth analysis of PPRV‐susceptible host species as well as the identified SLAM amino acid sequences to date. Investigation reveals that nine families—Bovidae, Camelidae, Cervidae, Elephantidae, Suidae, Felidae, Canidae, Muridae, and Ceratopogonidae—have been affected by PPRV infection. Furthermore, a bioinformatics‐based approach was proposed to screen the least common amino acid patterns (LCAP) in important SLAM receptor regions of known PPRV‐susceptible species. Research findings reveal that 14 least common amino acid sites (LCAS) in SLAM amino acid sequences (I61, I63, S60, S70, K76, K78, I79, S81, L82, E123, N125, S127, V128, and F131) exhibit a prevalent similarity to LCAP across all known susceptible species. Comparative analysis of these 14 LCAP with SLAM nucleotide sequences from unknown susceptible ruminants to identify species at heightened risk of PPRV. In the result, 48 species from 20 different families across six orders were at potential risk of being infected with PPRV. This exploration suggests the feasibility of assessing potential hosts at high risk of PPRV infection through the LCAS screening technique. Moreover, it offers a means to anticipate and issue warnings regarding the likelihood of interspecies transmission. In conclusion, this study integrates molecular biology and bioinformatics, shedding light on PPRV infection dynamics and paving the way for predictive strategies to prevent the spread of this devastating disease among ruminant populations.