MiRNAs are small non‐coding RNA molecules which serve as promising biomarkers due to their important roles in the development and progression of various cancer types. The detection of miRNAs is of vital importance to the early‐stage diagnostics and prognostics of multiple diseases. However, traditional detection strategies have faced some challenges owing to the intrinsic characteristics of miRNAs including small size, short sequence length, low concentration level and high sequence homology in complex real samples. To overcome these challenges, we proposed a MXene‐enhanced plasmonic biosensor for real‐time and label‐free detection of miRNA. By utilizing MXene nanomaterial which possesses unique characteristics including large surface area and strong carrier confinement abilities, we tuned the absorption of our plasmonic sensing substrate to reach a “zero‐reflection” state and induced an extremely sharp phase change at the resonance angle. Combined with the sensing mechanism based on phase‐induced lateral displacement measurement, this MXene‐enhanced plasmonic biosensor can achieve a much superior sensing performance compared to traditional SPR devices. Based on this biosensing scheme, the ultrasensitive detection of target miRNA with a detection limit down to 10 fM has been successfully demonstrated. More importantly, single‐base mismatched miRNA can be easily distinguished from the target miRNA according to the sensing signal. Furthermore, our plasmonic biosensor is capable of detecting miRNA in complex media such as 100 % human serum samples without compromising the detection sensitivity. This MXene‐enhanced plasmonic sensing scheme has the ability of detecting miRNAs with extremely low concentration levels in complex surrounding media without the need of introducing extra labels or amplification tags, which holds great potential in various biological applications and clinical diagnostics.

Digital droplet PCR (ddPCR) is a powerful amplification technique for absolute quantification of viral nucleic acids. Although commercial ddPCR devices are effective in the lab bench tests, they cannot meet current urgent requirements for on-site and rapid screening for patients. Here, we have developed a portable and fully integrated lab-on-a-disc (LOAD) device for quantitively screening infectious disease agents. Our designed LOAD device has integrated (i) microfluidics chips, (ii) a transparent circulating oil-based heat exchanger, and (iii) an on-disc transmitted-light fluorescent imaging system into one compact and portable box. Thus, droplet generation, PCR thermocycling, and analysis can be achieved in a single LOAD device. This feature is a significant attribute for the current clinical application of disease screening. For this custom-built ddPCR setup, we have first demonstrated the loading and ddPCR amplification ability by using influenza A virus-specific DNA fragments with different concentrations (diluted from the original concentration to 10⁷ times), followed by analyzing the droplets with an external fluorescence microscope as a standard calibration test. The measured DNA concentration is linearly related to the gradient–dilution factor, which validated the precise quantification for the samples. In addition to the calibration tests using DNA fragments, we also employed this ddPCR-LOAD device for clinical samples with different viruses. Infectious samples containing five different viruses, including influenza A virus (IAV), respiratory syncytial virus (RSV), varicella zoster virus (VZV), Zika virus (ZIKV), and adenovirus (ADV), were injected into the device, followed by analyzing the droplets with an external fluorescence microscope with the lowest detected concentration of 20.24 copies/µL. Finally, we demonstrated the proof-of-concept detection of clinical samples of IAV using the on-disc fluorescence imaging system in our fully integrated device, which proves the capability of this device in clinical sample detection. We anticipate that this integrated ddPCR-LOAD device will become a flexible tool for on-site disease detection.

In this paper, we report a simple, rapid, low-cost, biocompatible, and detachable microfluidic chip fabrication method for customized designs based on Parafilm^®. Here, Parafilm^® works as both a bonding agent and a functional membrane. Its high ultimate tensile stress (3.94 MPa) allows the demonstration of high-performance actuators such as microvalves and micropumps. By laser ablation and the one-step bonding of multiple layers, 3D structured microfluidic chips were successfully fabricated within 2 h. The consumption time of this method (~2 h) was 12 times less than conventional photolithography (~24 h). Moreover, the shear stress of the PMMA–Parafilm^®–PMMA specimens (0.24 MPa) was 2.13 times higher than that of the PDMS–PDMS specimens (0.08 MPa), and 0.56 times higher than that of the PDMS–Glass specimens (0.16 MPa), showing better stability and reliability. In this method, multiple easily accessible materials such as polymethylmethacrylate (PMMA), PVC, and glass slides were demonstrated and well-incorporated as our substrates. Practical actuation devices that required high bonding strength including microvalves and micropumps were fabricated by this method with high performance. Moreover, the biocompatibility of the Parafilm^®-based microfluidic devices was validated through a seven-day E. coli cultivation. This reported fabrication scheme will provide a versatile platform for biochemical applications and point-of-care diagnostics.

Droplet microfluidics is an emerging tool in many biological and chemical application areas such as digital polymerase chain reaction (PCR) and in vitro diagnosis because of its extremely small sample volume and wide range of possibilities for on-demand adjustment of droplet properties. Although centrifugal microfluidics has been reported as a viable scheme for droplet generation, there is not much progress as far as droplet manipulation and droplet-based reactions are concerned. In this paper, we report a microfluidic pressure regulator scheme along with the use of microcapillaries for periodic droplet generation and the subsequent fusion. This scheme enables fine control over droplet generation and the fusion process by varying the rotational frequency. To control the solution concentration in droplets, we have implemented several fusion devices, including one-to-one mode using a symmetric structure and ratio-adjustable mode with an asymmetric structure. As an application example, we performed cell transfection using the reported droplet-based technique, which resulted in considerable improvement in terms of transfection efficiency compared to the traditional bulk approach. In another example, we synthesized quasi-2D perovskites with controllable compositions and tunable photoluminescence peaks, thus confirming the volumetric accuracy of this approach down to the nano-liter scale. Compared to the common pressure pulsation approach, our centrifugal force actuation scheme offers the advantages of compactness and highly parallel batch processing. We anticipate that the new scheme will find many applications in cell biology and chemical synthesis.

Based on the optical trapping force of an evanescent wave at a micro-ring resonator alongside a waveguide, we propose a tunable optofluidic sorting unit for micro-nanoparticles by localized thermal phase tuning. With the tuning of field build-up factor of resonator, the depth of trapping potential well and the size of trapped particle are adjustable. Furthermore, by considering the Brownian motion of trapped particles from a statistics perspective, we verify the critical trapping threshold of a potential well, which is usually assumed to be $1k_{B}T$. The threshold depends not only on the optical power and particle size, but also on the length of the coupling region. Compared with a wavelength tuning mechanism, localized thermal tuning enables large-scale integration of many independent tunable resonators. As a demonstration, we propose a set of operations with three resonators for nanoparticle manipulation, including sorting, storing, and mixing. Our proposed function units are of great importance for on-chip large-scale integration of optofluidic systems.