The increase in the world population has generated an important need for both quality and quantity agricultural products, which has led to a significant surge in the use of chemical pesticides to fight crop diseases. Consumers, however, have become very concerned in recent years over the side effects of chemical fungicides on human health and the environment. As a result, research into alternative solutions to protect crops has been imposed and attracted wide attention from researchers worldwide. Among these alternatives, biological controls through beneficial microorganisms have gained considerable importance, whilst several biological control agents (BCAs) have been screened, among them Bacillus, Pantoea, Streptomyces, Trichoderma, Clonostachys, Pseudomonas, Burkholderia, and certain yeasts. At present, biopesticide products have been developed and marketed either to fight leaf diseases, root diseases, or fruit storage diseases. However, no positive correlation has been observed between the number of screened BCAs and available marketed products. Therefore, this review emphasizes the development of biofungicides products from screening to marketing and the problems that hinder their development. Finally, particular attention was given to the gaps observed in this sector and factors that hamper its development, particularly in terms of efficacy and legislation procedures.

Grapevine trunk diseases (GTD) are currently one of the most devastating and challenging diseases in viticulture, leading to considerable yield losses and a remarkable decline in grapevine quality. The identification of the causal agents is the cornerstone of an efficient approach to fighting against fungal diseases in a sustainable, non-chemical manner. This review attempts to describe and expose the symptoms of each pathology related to GTD, the modes of transmission, and the harmfulness of recently reported agents. Special attention was given to new diagnostic tests and technologies, grapevine defense mechanisms, molecular mechanisms of endophytes fungal colonization, and management strategies used to control these threats. The present extended review is, therefore, an updated state-of-the-art report on the progress in the management of vineyards.

Canker symptoms were observed on almond trees in the Fez-Meknes region, Morocco. Isolations were conducted from the infected branch and trunk showing internal and external brown lesions. Four representative fungal isolates were screened, and their identities were confirmed by cultural traits and sequence analysis of DNA using two genes, the ITS region (internal transcribed spacer) and calmodulin (cmdA). The identified fungi were Curvularia hawaiiensis, Fusarium ambrosium, Lasiodiplodia theobromae, and Chondrestereum purpureum. The pathogenicity test on almond twigs revealed that these species were pathogenic to their host with different degrees of virulence, with Lasiodiplodia theobromae as the most virulent causing the longest necrotic lesion (285.17 mm) and the death of twigs. Physiological traits analysis of the above-mentioned fungi showed that the optimum mycelium growth response at different temperatures varies from 10 to 35 °C, while the pH ranges between 3.0 and 8.0. This study confirms the presence of canker pathogens on almond trees, which will contribute valuable information to improve the understanding of the contemporary status of almond trees, thus helping the improvement of the management of almond orchards. To our knowledge, all these fungi represent new records in Morocco and some of them are confirmed on the almond trees for the first time in the world.

Citrus fruits are vulnerable to green mold (caused by Penicillium digitatum) and blue mold (caused by Penicillium italicum) during storage, posing significant challenges to the industry. Therefore, biological control utilizing antagonistic bacteria has emerged as a dependable strategy for managing postharvest diseases. In this study, halophilic bacterial isolates were carefully selected from diverse saline ecosystems, including the Dead Sea, the Agadir Sea, the Rabat Sea, saline soil, and water of the Amassine Oued in Taounate, based on rigorous in vitro and in vivo antagonism bioassays. Out of 21 bacteria from different saline environments, 10 were chosen for further characterization based on the 16S rDNA gene. Notably, the EAM1 isolate demonstrated exceptional inhibitory effects, reaching a 90% inhibition rate against P. digitatum, while the ER2 isolate closely followed with an 89% inhibition rate against P. italicum. Furthermore, in bacterial supernatant experiments, six bacterial isolates effectively curbed the growth of P. digitatum, and three demonstrated efficacy against P. italicum development. In an in vivo trial spanning ten days of incubation, three highly effective isolates against P. digitatum displayed zero severity, and two of these isolates also demonstrated zero severity against P. italicum. Interestingly, a comparison of bacterial filtrates revealed that all isolates exhibited a severity level of over 50% against the pathogen causing green rot (P. digitatum), while the severity was lower than 50% for the supernatants of the two isolates used against P. italicum. In conclusion, this study highlights the promising role of halophilic bacteria, specifically Bacillus amyloliquefaciens EAM1 and B. amyloliquefaciens ER2, in controlling postharvest fruit pathogens. The findings shed light on the potential of utilizing these bioprotective agents to address the challenges posed by green and blue citrus molds, providing valuable insights for the citrus industry.

A decline of various grapevines (Vitis vinifera L.) in the province of Doukkala in Morocco was observed in 2021. The causal pathogen was identified as Lasiodiplodia viticola based on morphological characteristics and phylogenetic analysis of the internal transcribed region (ITS), the β-tubulin gene (TUB) and calmodulin (cmdA). Koch’s postulates were confirmed by successful re-isolation of L. viticola from plants inoculated with the pathogen under controlled conditions. The disease was shown to be prevalent in Bni Hilal (71.43%), Laamria (60%), and Boulaouane (40%) districts, but was quasi-absent in Lmechrek. To understand the dominance of L. viticola as one of the grapevine trunk pathogens, effects of temperature (10–40 °C) and pH (pH 3–pH 12) on growth and sporulation were investigated. The species were able to grow in a range of temperatures ranging from 15 to 40°C and showed a higher growth rate at 35 °C. The fungus were also characterized by a broad optimum pH ranging between 3–12. This study is the first report dealing with L. viticola associated with grapevine trunk diseases in Morocco. Additional studies are therefore required to understand the high occurrence of this disease in vineyards, which is likely due to climate changes. A good understanding of this complex disease might help to develop a reliable and sustainable preventive control strategy.