Some studies revealed that thermography is a noninvasive diagnostic technic that can detect body heat emission through infrared radiation and determine the superficial body temperature of the animal (
Stewart et al., 2005). Physical exercise produces net heat since only 20 to 25% of the energy used by the muscle is converted to mechanical energy (
Hyyppa and Poso, 2004). During exercise, metabolic heat production increases with increasing exercise intensity (
Mckeever, 2004). To understand exercise and the influence of exertion on thermographic images of clinically normal horses requires using thermography appropriately (
Simon et al., 2006). In this context, the present study used thermography to evaluate whether the superficial temperature of upper fore and hindlimbs of Mangalarga horses increased as a result of heat production during a four-beat gait exercise at 15km.h
-1 for 20 minutes.