Metal nanoparticles (NPs), with sizes ranging from 1–100 nm, are of great scientific interest because their functions and features differ greatly from those of bulk metal. Chemical or physical methods are used to synthesize commercial quantities of NPs, and green, energy-efficient approaches generating byproducts of low toxicity are desirable to minimize the environmental impact of the industrial methods. Some microorganisms synthesize metal NPs for detoxification and metabolic reasons at room temperature and pressure in aqueous solution. Metal NPs have been prepared via green methods by incubating microorganisms or cell-free extracts of microorganisms with dissolved metal ions for hours or days. Metal NPs are analyzed using various techniques, such as ultraviolet-visible spectroscopy, electron microscopy, X-ray diffraction, electron diffraction, energy-dispersive X-ray spectroscopy, and X-ray photoelectron spectroscopy. Numerous publications have focused on microorganisms that synthesize various metal NPs. For example, Ag, Au, CdS, CdSe, Cu, CuO, Gd₂O₃, Fe₃O₄, PbS, Pd, Sb₂O₃, TiO₂, and ZrO₂ NPs have been reported. Herein, we review the synthesis of metal NPs by microorganisms. Although the molecular mechanisms of their synthesis have been investigated to some extent, experimental evidence for the mechanisms is limited. Understanding the mechanisms is crucial for industrial-scale development of microorganism-synthesized metal NPs.

Gold nanoparticles have particular properties distinct from those of bulk gold crystals, and such nanoparticles are used in various applications in optics, catalysis, and drug delivery. Many reports on microbial synthesis of gold nanoparticles have appeared. However, the molecular details (reduction and dispersion) of such synthesis remain unclear. In the present study, we studied gold nanoparticle synthesis by Lactobacillus casei. A comparison of L. casei components before and after addition of an auric acid solution showed that the level of unsaturated lipids decreased significantly after addition. NMR and mass spectrum analysis showed that the levels of diglycosyldiacylglycerol (DGDG) and triglycosyldiacylglycerol (TGDG) bearing unsaturated fatty acids were much reduced after formation of gold nanoparticles. DGDG purified from L. casei induced the synthesis of gold nanoparticles in vitro. These results suggested that glycolipids, such as DGDG, play important roles in reducing Au(III) to Au(0) and in ensuring that the nanoparticles synthesized remain small in size. Our work will lead to the development of novel, efficient methods by which gold nanoparticles may be produced by, and accumulated within, microorganisms.

CdSe quantum dots are often used in industry as fluorescent materials. In this study, CdSe quantum dots were synthesized using Fusarium oxysporum. The cadmium and selenium concentration, pH, and temperature for the culture of F. oxysporum (Fusarium oxysporum) were optimized for the synthesis, and the CdSe quantum dots obtained from the mycelial cells of F. oxysporum were observed by transmission electron microscopy. Ultra-thin sections of F. oxysporum showed that the CdSe quantum dots were precipitated in the intracellular space, indicating that cadmium and selenium ions were incorporated into the cell and that the quantum dots were synthesized with intracellular metabolites. To reveal differences in F. oxysporum metabolism, cell extracts of F. oxysporum, before and after CdSe synthesis, were compared using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The results suggested that the amount of superoxide dismutase (SOD) decreased after CdSe synthesis. Fluorescence microscopy revealed that cytoplasmic superoxide increased significantly after CdSe synthesis. The accumulation of superoxide may increase the expression of various metabolites that play a role in reducing Se⁴⁺ to Se²⁻ and inhibit the aggregation of CdSe to make nanoparticles.