Induction of defense-related enzymes, such as phenylalanine ammonia lyase (PAL), peroxidase (PO), polyphenol oxidase (PPO), superoxide dismutase (SOD) and catalase (CAT) due to bacterial antagonists viz., Pseudomonas fluorescens (Pf1) and Bacillus subtilis (EPCO16) and plant-derived lipoxygenase volatile compound hexanal, were studied in mango fruits against Lasiodiplodia theobromae causing stem-end rot disease. The results showed increased induction of all the defense-related enzymes in mango fruits 3–5 days after dipping treatment with combination of bacterial antagonists and hexanal when compared to untreated control treatment and treatment with fungicide carbendazim in storage condition. The increased activity was observed up to 3 days after treatment and thereafter declined. Further, increased expression of specific isoforms of PO, PPO, SOD and CAT were also observed in the treatment effect of P. fluorescens (0.5%) + hexanal (0.02%) treated fruits against L. theobromae. From the results obtained, it is inferred that due to the enhancement of defense-related enzymes via the phenylpropanoid pathway and due to secretion of secondary metabolites that would play significant role in hindering the pathogen quiescence and further invasion in mango fruits and thereby prevent the fruit rot.

The global COVID-19 pandemic has hit the agriculture sector hard around the world. A study was conducted to assess the impact of the pandemic on cropping patterns, crop management, usage of chemical inputs and their organic alternatives, harvesting, and marketing avenues through a survey approach in the two states of Kerala and Tamil Nadu in India. A total of 250 farmers participated in the study, the data was analyzed by Chi-square test and Kruskal–Wallis test. The assessment of the impact of COVID-19 on some aspects was undertaken by dividing the study period into three phases. Though a smaller number of people were infected with COVID-19 in the initial phase of the pandemic compared to the later phases, farm operations and the procurement of inputs were significantly affected at this phase as there was a sudden disruption in transportation due to COVID-19-induced movement restrictions. During the entire study period, commodities such as rice, bananas, vegetables, coconuts, and flowers suffered maximum crop loss compared to pulses, groundnuts, cotton, and rubber. Among fertilizers, the maximum shortage was observed for chemical fertilizers (46%) and biofertilizers (30%) compared to cow dung (18%) and poultry manure (6%), indicating that farmers tended to use more local materials that could be easily procured and accessed compared to shop-based inputs. A rise in the cost of cultivation, scarcity of farm workforce, and difficulty in hiring farm machinery all have contributed to the loss of profit during the pandemic period. As a response to COVID-19, growers initiated post-harvest processing of commodities, and cropping systems remained the same during the period. The paper also discusses some remedial measures to be adopted by households in the future, to minimize the impacts of such pandemics in the agrarian sector.

Kappaphycus alvarezii (Doty) L. M. Liao, a red seaweed widely cultivated for carrageenan polysaccharide, is also a potential source of the valuable pigment phycoerythrin (PE). Therefore, this study aims to extract phycoerythrin from K. alvarezii, evaluate its antimicrobial, antioxidant, and anticancer activities, and identify its biomedical potential for future therapeutic applications. The protein content of phycoerythrin pigment extracted from K. alvarezii was found to be 69.84% and showed excellent antimicrobial activity against Klebsiella oxytoca and Proteus mirabilis, with a minimum inhibition zone of 11 mm. It showed significant in vitro antioxidant activity, as analyzed using total antioxidant, hydrogen peroxide scavenging, reducing power, DPPH, and ABTS assays. Further, the pigment exhibited potent cytotoxicity against a human lung cancer cell line, with an IC₅₀ value of 131.7 µg mL⁻¹. Furthermore, increasing concentration of phycoerythrin pigment decreased the cell proliferation and induced apoptosis, as confirmed by Annexin V/PI staining. Comprehensive characterization using FT-IR, HPLC, and GC-MS analysis revealed the nature of pigment and functional groups, highlighting its potential for biomedical applications. The molecular docking of K. alvarezii-derived compounds revealed significant binding affinities with 13 antibacterial target proteins. These results highlight the potential of K. alvarezii bioactive compounds as promising antibacterial agents. The phycoerythrin extract from K. alvarezii demonstrated potent antimicrobial, antioxidant, and anticancer properties, with significant cytotoxicity against lung cancer cells and confirmed apoptosis induction. Structural analysis revealed its bioactive composition, emphasizing its potential as a natural therapeutic agent. These findings support its potential application in the biomedical and pharmaceutical industries.

This study investigates the specific challenges and opportunities faced by organic farmers in Tamil Nadu, focusing on how region-specific practices influence the adoption and success of organic farming. It draws insights from a survey of 300 organic growers across five districts: Coimbatore, Dindigul, Erode, Tirupur, and Villupuram. This study is particularly relevant because there is a lack of comprehensive research on the specific challenges and opportunities faced by organic farmers in Tamil Nadu, with limited data on the long-term impact of government initiatives and region-specific management options, especially for smallholder farmers. Additionally, gaps exist in understanding the economic viability, market demand, and farmer perceptions of organic farming together with challenges like knowledge gaps, pest control, managing risks, and lower initial yields during the transition from conventional farming to organic farming. The research evaluated the factors affecting organic farming, including crop yield, the use of different organic formulations, the role of different information services, opportunities and challenges, and the impact on the perceived effectiveness of various agricultural outcomes. Various statistical analyses were performed, including K-means clustering, Poisson regression, and chi-square test. The results revealed significant variations in the adoption of various organic inputs and regionally practiced organic formulations. Crop residues (82.67%), Jeevamrith (78.33%), Amudhakaraisal (77.00%), and 3G-Extracts (74.00%) have high adoption rates, reflecting their perceived effectiveness and accessibility among farmers. However, the adoption of microbial bio-inputs such as Trichoderma viride (1.00%), Acetobacter spp. (19.00%), Azophos (consortium of phosphorus fixing microbes) (26.33%), and Azospirillum spp. (28.67%) was notably poor despite their known benefits for soil health and crop yield. The chi-square test also highlighted key challenges faced by farmers in adopting organic farming practices. The Poisson regression analysis showed significant positive impacts on crop growth and yield, disease resistance, insect control, overall plant health, and input efficiency due to organic farming. The study’s insights are essential for developing targeted strategies to overcome barriers, supporting the growth and sustainability of organic farming. Addressing these challenges and promoting effective information channels will better equip stakeholders to support organic growers, contributing to more resilient and productive agricultural systems in the region.