Waste glycerol generated during biofuel production accounts for ~10% of total biodiesel volume. Increasing the use of renewable energy sources, including so-called biodiesel, will significantly increase the amount of waste glycerol for disposal. One possible route for waste glycerol reuse is to use it as a draw solution in forward osmosis (FO). Glycerol solutions are particularly suited as FO draw solutions due to their high osmotic pressures. In this work, the effects of waste glycerol composition on FO draw solution osmotic pressures, as well as the effects of membrane type and linear flow velocities on FO water and reverse flux, were investigated. Those results indicated the feasibility of using waste glycerol as a draw solution in FO, allowing the reuse of significant amounts of this by-product.