In this study, we investigated the mechanism of photosynthesis and physiological function of blueberry leaves under low temperature stress (4–6°C) by exogenous hydrogen sulfide (H₂S) by spraying leaves with 0.5 mmol·L^–1 NaHS (H₂S donor) and 200 μmol·L^–1 hypotaurine (Hypotaurine, H₂S scavenger). The results showed that chlorophyll and carotenoid content in blueberry leaves decreased under low temperature stress, and the photochemical activities of photosystem II (PSII) and photosystem I (PSI) were also inhibited. Low temperature stress can reduce photosynthetic carbon assimilation capacity by inhibiting stomatal conductance (G_s) of blueberry leaves, and non-stomatal factors also play a limiting role at the 5^th day of low temperature stress. Low temperature stress leads to the accumulation of Pro and H₂O₂ in blueberry leaves and increases membrane peroxidation. Spraying leaves with NaHS, a donor of exogenous H₂S, could alleviate the degradation of chlorophyll and carotenoids in blueberry leaves caused by low temperature and reduce the photoinhibition of PSII and PSI. The main reason for the enhancement of photochemical activity of PSII was that exogenous H₂S promoted the electron transfer from Q_A to Q_B on PSII acceptor side under low temperature stress. In addition, it promoted the accumulation of osmotic regulator proline under low temperature stress and significantly alleviated membrane peroxidation. H₂S scavengers (Hypotaurine) aggravated photoinhibition and the degree of oxidative damage under low temperature stress. Improving photosynthetic capacity as well as alleviating photosynthetic inhibition and oxidative stress with exogenous H₂S is possible in blueberry seedlings under low temperature stress.

Experiences in natural environments can enhance human wellbeing and promote the recovery of physiological and psychological health. During the COVID-19 pandemic, university students' activity off-campus was limited, thus, the campus environment was particularly important for the recovery of students' physiological and psychological health. Although the benefits of sustained natural exposure are obvious for people's physiological and psychological health, the effects of brief exposure on physiological and psychological are unclear. In the present study, four types of campus environments, including square space, dense forest space, sparse forest space, and waterfront space, were selected to explore the transient recovery effects of different types of campus environments. Sixty university students were recruited, measuring their systolic blood pressure, diastolic blood pressure, and heart rate as physical parameters to assess stress recovery. Respondents also reported scores about their personal feelings in questionnaires to evaluate their psychological states. Both physiological and psychological indicators responded to the brief natural exposure (5 min), and physiological and psychological health was restored. However, only the recovery amounts of psychological indicators were significantly different in waterfront space, dense forest space, and sparse forest space. These results indicate that being compare with other spaces, the brief exposure in the waterfront space was the most beneficial to students' psychological health recovery. This recovery was attributed to the great role played by the sense of escape, but after the brief exposure, the attraction and compatibility of the environment would hinder the psychological health recovery. In conclusion, according to tests on both physiological and psychological aspects, the waterfront environment on campus is the best choice for students' transient health recovery.

As an ornamentally and medicinally worthy plant, Hosta plantaginea (Lam.) Aschers. has the adapted capacity to survive cold temperate monsoon climates in Northeastern China. However, its use is limited by the soil alkalization of urban gardens. Our pre-experiment found that Hosta ‘Golden Cadet’ has the potential to be alkali-tolerant. Hence, tissue-cultured seedlings of Hosta ‘Golden Cadet’ were used as experimental material. Its related growth, physiology, and transcripts were examined to reveal the molecular mechanism of Hosta plantaginea in response to alkali stress. The results show that the development of Hosta ‘Golden Cadet’ was affected by alkali stress. In comparison with the control, malondialdehyde (MDA) content increased by 4.28-fold at the 24th hour, superoxide dismutase (SOD) activity increased by 49% at the 6th hour, and peroxidase (POD) activity and soluble sugar (SS) content increased by 67% and 30% at the 12th hour, respectively. The RNA-seq analysis revealed that Hosta ‘Golden Cadet’ gene expressions at 0 h, 6 h, 12 h, 21 h and 48 h differed after 200 mmol/L NaHCO₃ treatment. During 48 h under alkali stress, 2366 differentially expressed genes were found. The transcription factors MYB, AP2/ERF, and WRKY were activated in differentially expressed genes. The KEGG analysis found that phytohormone signaling pathways, starch and sucrose metabolism, and phenylpropane production were activated in Hosta ‘Golden Cadet’ in response to alkali stress. In summary, Hosta ‘Golden Cadet’ can reduce membrane damage by improving osmoregulation and antioxidant capacity, increase sucrose and starch metabolism, and regulate phenylpropane biosynthesis by activating transcription factors and inducing related phytohormone signaling, mitigating the effects of alkali toxicity. These findings guide an investigation into the mechanism of alkali tolerance in Hosta plants, screening alkali tolerance genes, and selecting and breeding novel alkali-tolerant Hosta plantaginea cultivars.