Soil aggregation plays a critical role in the maintenance of soil structure and crop productivity. Fertilization influences soil aggregation, especially by regulating soil organic carbon (SOC) and total nitrogen (TN) contents in aggregate fractions. Here, we conducted a fixed-site field experiment to quantify the effect of five N application rates: 0, 75, 150, 225, and 300 kg·N·ha⁻¹, denoted as N0, N75, N150, N225, and N300, respectively, on soil aggregate stability, aggregate-associated SOC and TN sequestration and crop productivity. Soil aggregates were divided into >0.25 (>5, 5–2, 2–1, 1–0.5, 0.5–0.25) and 0.25 mm), decreased the proportion of micro-aggregates (5 mm macro-aggregates in N225 was significantly increased by 19.24% under dry sieving (p < 0.05). With the increase in N application rate, the bulk SOC and TN contents first increased and then decreased, and the SOC and TN of N225 were significantly increased by 17.75% and 72.33% compared with N0 (p < 0.05). More specifically, fertilization promoted the distribution and enrichment of SOC and TN in macro-aggregates and reduced the C/N of the micro-aggregates and the contribution of SOC and TN in the micro-aggregates. Compared with N0, the contribution rate of macro-aggregates to SOC and TN of N225 under wet sieving was significantly increased by 84.13 and 17.18%, respectively, while the C/N of micro-aggregates of N225 under wet and dry sieving methods was significantly decreased by 45.95 and 31.74%, respectively (p < 0.05). Moreover, fertilization improved the yield, and N225 significantly increased the total yield by 80.68% compared with N0 (p < 0.05). In conclusion, N225 was the suitable N application for improving soil aggregate stability, carbon and nitrogen sequestration, and crop productivity on the Loess Plateau, China.

Deep fertilization is a beneficial approach for reducing nitrogen losses. However, the effects of various fertilization depths on maize (Zea mays L.) productivity and environmental footprints have not been thoroughly understood. Therefore, a field experiment was conducted to investigate the effects of different fertilization depths of 5 cm (D5), 15 cm (D15), 25 cm (D25), and 35 cm (D35) on maize productivity and environmental footprints. Reactive nitrogen (Nr) losses and greenhouse gas (GHG) emissions were assessed using life cycle analysis. We hypothesized that deep fertilization can obtain lower carbon and nitrogen footprint. The results indicated that deep fertilization decreased the N₂O and NH₃ emissions while increasing the CH₄ uptake. Compared with D5, D15 resulted in an increase in total GHG emissions and carbon footprint (CF), whereas D25 decreased by 13.0% and 23.6%, respectively. Compared with D5, the Nr losses under D15, D25, and D35 conditions was reduced by 11.3%, 17.3%, and 21.0%, respectively, and the nitrogen footprint (NF) was reduced by 16.0%, 27.4%, and 19.0%, respectively. The maize yield under D15 and D25 increased by 5.7% and 13.8%, respectively, compared with the D5 treatment, and the net economic benefits of the ecosystem increased by 7.1% and 17.1%, respectively. In summary, applying fertilizer at a depth of 25 cm can significantly reduce the environmental footprints and increase maize productivity, making it an effective fertilization strategy in the Loess Plateau region of China.

Low solar radiation, caused by climate change or dense planting patterns, now limits wheat production. Although wheat breeding has increased lodging resistance and yield potential through the introduction of dwarfing genes, it still reduces wheat yields. Few studies have been conducted to clarify the lodging sensitivity to shading of different-era wheat cultivars in China’s Huang-Huai-Hai region, as well as the characteristics of lodging resistance as affected by paclobutrazol under shading stress. To address this gap, the experiment included two wheat cultivars released in different decades, grown under shade and treated with or without paclobutrazol. The results showed that reductions in filling degree and lignin content, together with increases in length of the basal internode and gravity center height, markedly reduced the section modulus and breaking strength of shaded wheat culms. These changes impaired lodging resistance and raised lodging risk. However, paclobutrazol application effectively reduced lodging incidence and increased wheat yield under shading stress. Furthermore, these responses were more pronounced in the old cultivar (YZM) than in the modern cultivar (S28). This indicates that the culm mechanical parameters of the old cultivar were more shade-sensitive than those of the modern cultivar. Moreover, shading downregulated the relative expression levels of key genes associated with lignin biosynthesis to decrease the activities of key enzymes, thereby inhibiting the biosynthesis and deposition of lignin in culms to increase the risk of wheat lodging. Paclobutrazol application alleviated the inhibitory effects of shading on lignin biosynthesis, thereby strengthening culms and enhancing lodging resistance. These findings may provide a basis for exploring cultivation regulation methods to enhance wheat lodging resistance under overcast and low-sunshine conditions, and to offer guidance for the breeding of wheat cultivars with lodging resistance and shade tolerance.

Knowledge of the characteristics of N₂O emissions and the influential mechanism is of great significance to mitigate greenhouse gas emissions in semi-arid areas. In the present study, a three-year water-control study was conducted; three simulated rainfall amounts (heavy, normal, and light rainfall = 275, 200, and 125 mm, respectively), two wheat (Triticum aestivum L.) planting modes (RF (ridge–furrow mulching system) and TF (traditional flat planting)) and four supplementary irrigation amounts (150, 75, 37.5, and 0 mm) were set up. The effects of different cultivation methods and irrigation amounts on soil N₂O emissions, the soil water content, available nitrogen content, and denitrifying enzyme activity were investigated to clarify the N₂O emission mechanism in winter wheat fields (Triticum aestivum L.). The results obtained after three years showed that compared with TF, the N₂O emissions under RF decreased by 21.62–30.72% (p < 0.001), whereas the soil water content increased by 6.26–8.82%, the available nitrogen content decreased by 1.71–16.24%, and the denitrifying enzyme activities increased by 0.2–24.16% under heavy rainfall conditions. Under conditions with normal and light rainfall, the N₂O emission fluxes under RF increased by 3.66–12.46% and 6.08–15.57% (p > 0.05), while the soil water contents increased by 6.13–11.49% and 8.05–13.88%, the soil available nitrogen contents decreased by 11.0–21.42% and 19.93–34.44%, and the denitrifying enzyme activities increased by 0.01–24.08% and 0.03–20.79% compared with TF. Principal component analysis showed that the main factors related to N₂O emissions under RF were the soil moisture content and available nitrogen content; these factors combined explained 94.37% the variation of the N₂O emissions. However, the main factors under TF were the soil moisture content and denitrifying enzyme activity; these factors combined explained 85.81%. In the heavy and normal rainfall years, compared with TF, using RF and 75 mm irrigation achieved the goal of reducing water usage as well as decreasing the N₂O emissions (or N₂O increase was not significant). In light rainfall years, RF with 150 mm irrigation obtained significant reductions in water usage compared with TF but it also increased the N₂O emission flux. Under different rainfall years, the yield of RF increased by 2.89–50.44% compared with the TF system, and the increase in wheat grain yield increased with decreasing rainfall.

The ridge–furrow rainwater harvesting (RFRH) planting mode is widely used in arid and semi-arid areas to solve the problems of agricultural water shortage and low productivity. However, the impact of film mulching on the stability of soil carbon pools makes this planting mode vulnerable to the risk of increased soil carbon emissions and carbon pool losses. In order to clarify the relationship between soil carbon emissions and hydrothermal factors, as well as the regulatory effect of biochar application on soil carbon sequestration and reduced emissions under this planting mode, we set up a biochar application experiment. The effects of the biochar application (at 10 Mg ha⁻¹ biochar and 20 Mg ha⁻¹ biochar) on the soil water dynamics, soil temperature changes, CO₂-C and CH₄-C flux dynamics, grain yield, carbon emission efficiency, and the net ecosystem carbon budget in wheat fields under the RFRH planting mode were investigated, with no biochar application as the control. The results showed that applying biochar increased the soil water content, soil average temperature, cumulative CH₄-C uptake, wheat grain yield, and carbon emission efficiency by 3.10–12.23%, 0.98–3.53%, 59.27–106.65%, 3.51–16.42%, and 18.52–61.17%, respectively; reduced the cumulative CO₂-C emissions by 7.51–31.07%; and increased the net ecosystem carbon budget by 2.91 Mg C ha⁻¹ to 6.06 Mg C ha⁻¹. The results obtained by equation fitting showed that in wheat fields under RFRH, the CO₂-C emission fluxes had negative and positive exponential relationships with the soil water content and soil temperature, respectively, while the CH₄-C uptake fluxes had no significant correlation with the soil water content and had an inverse U-shaped quadratic function relationship with soil temperature. Overall, these results suggest that the application of biochar to wheat fields under RFRH can improve grain yield, farmland carbon emission efficiency, and the net ecosystem carbon budget, and change wheat fields from a carbon source to a carbon sink. These results can provide a theoretical basis and technical support for efficient, green, and sustainable production in farmland in arid and semi-arid areas.