Hepatitis B has been one of the most prevalent infectious diseases in the world and specifically in Indonesia. Although the total conversion of hepatitis B virus (HBV) to chronic disease in Indonesia was reduced by 50%, the total number of hepatitis B cases increased by 2.5 times in 2021. Ineffective HBV immunization program in Indonesia prior to 1997 was addressed by the Ministry of Health through a more comprehensive HBV control, which, among others, involved Health Promotion to increase people’s knowledge and awareness towards hepatitis B infection prevention. In this regard, this study aims to identify the level of knowledge, attitude, and practice/behavior of the Indonesian population towards hepatitis B infection prevention and their willingness for screening, particularly in areas with high prevalence of hepatitis B. This study used a quantitative approach in looking at correlations between this set of knowledge, attitude, and practice, mainly by using Structural Equation Model (SEM) and SmartPLS 3.3.3 toolkit in SPSS. Through an analysis of online questionnaire results from over 400 respondents in four provinces (DKI Jakarta, West Java, DI Yogyakarta, and South Sulawesi), this study shows that respondents have a very high level of knowledge about hepatitis B; high level of positive attitude; and very high level of positive practice towards hepatitis B infection prevention. However, we also noticed some dissonances between the key aspects, namely that knowledge on hepatitis B correlates negatively with the behavior of the respondents and that the behavior also correlates negatively with their willingness for screening. In conclusion, we suggest that factors such as socio-economic context and prior informed knowledge on hepatitis B be considered to build a better strategy of Health Promotion and hepatitis B diagnostic screening among the population.