The present study explored the eco-friendly approach of utilizing plant-growth-promoting rhizobacteria (PGPR) inoculation and foliar application of silicon (Si) to improve the physiology, growth, and yield of mung bean under saline conditions. We isolated 18 promising PGPR from natural saline soil in Saudi Arabia, and screened them for plant-growth-promoting activities. Two effective strains were selected from the screening trial, and were identified as Enterobacter cloacae and Bacillus drentensis using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry and 16S rRNA gene sequencing techniques, respectively. Subsequently, in a 2-year mung bean field trial, using a randomized complete block design with a split-split plot arrangement, we evaluated the two PGPR strains and two Si levels (1 and 2 kg ha-1), in comparison with control treatments, under three different saline irrigation conditions (3.12, 5.46, and 7.81 dS m-1). The results indicated that salt stress substantially reduced stomatal conductance, transpiration rate, relative water content, total chlorophyll content, chlorophyll a, chlorophyll b, carotenoid content, plant height, leaf area, dry biomass, seed yield, and salt tolerance index. The PGPR strains and Si levels independently improved all the aforementioned parameters. Furthermore, the combined application of the B. drentensis strain with 2 kg Si ha-1 resulted in the greatest enhancement of mung bean physiology, growth, and yield. Overall, the results of this study provide important information for the benefit of the agricultural industry.

Plant growth promoting rhizobacteria (PGPR) and silicon (Si) are known for alleviating abiotic stresses in crop plants. In this study, Bacillus drentensis and Enterobacter cloacae strains of PGPR and foliar application of Si were tested for regulating the antioxidant metabolism and nutrient uptake on grain yield of mung bean under irrigation of saline water (3.12 and 7.81 dS m⁻¹). Bacterial inoculation and supplemental Si (1 and 2 kg ha⁻¹) reduced salinity-induced oxidative stress in mung bean leaves. The improved salt stress tolerance was achieved by enhancing the activities of catalase (45%), peroxidase (43%) and ascorbate peroxidase (48%), while decreasing malondialdehyde levels (57%). Enhanced nutrient uptake of magnesium 1.85 mg g⁻¹, iron 7 mg kg⁻¹, zinc 49.66 mg kg⁻¹ and copper 12.92 mg kg⁻¹ in mung bean seeds was observed with foliar application of Si and PGPR inoculation. Biomass (7.75 t ha⁻¹), number of pods per plant (16.02) and 1000 seed weight (60.95 g) of plants treated with 2 kg Si ha⁻¹ and B. drentensis clearly outperformed treatments with Si or PGPR alone. In conclusion, application of Si and PGPR enhances mung bean productivity under saline conditions, thereby helping exploitation of agriculture in low productive areas.