Zhenxi Zhang

Dr. Zhenxi Zhang


Research Focus & Metrics

Academic Career Length

25Years (2002-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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1 January 2005 in Journal of Biomedical Optics
Verified
Journal Article
Closed access
PDF file
CYCuiping Yao
Cuiping Yao
RRRamtin Rahmanzadeh
Ramtin Rahmanzadeh
EEElmar Endl
Elmar Endl
ZZZhenxi Zhang
Zhenxi Zhang
JGJohannes Gerdes
Johannes Gerdes
GHGereon Hüttmann
Gereon Hüttmann
Irradiation of nanoabsorbers with pico- and nanosecond laser pulses could result in thermal effects with a spatial confinement of less than 50nm. Therefore absorbing nanoparticles could be used to create controlled cellular effects. We describe a combination of laser irradiation with nanoparticles, which changes the plasma membrane permeability. We demonstrate that the system enables molecules to penetrate impermeable cell membranes. Laser light at 532nm is used to irradiate conjugates of colloidal gold, which are delivered by antibodies to the plasma membrane of the Hodgkin’s disease cell line L428 and/or the human large-cell anaplastic lymphoma cell line Karpas 299. After irradiation, membrane permeability is evaluated by fluorescence microscopy and flow cytometry using propidium iodide (PI) and fluorescein isothiocyanate (FITC) dextran. The fraction of transiently permeabilized and then resealed cells is affected by the laser parameter, the gold concentration, and the membrane protein of the different cell lines to which the nanoparticles are bound. Furthermore, a dependence on particle size is found for these interactions in the different cell lines. The results suggest that after optimization, this method could be used for gene transfection and gene therapy.

1 January 2019
Verified
Journal Article
Closed access
HLHuifang Liu
Huifang Liu
SWSijia Wang
Sijia Wang
JXJing Xin
Jing Xin
JWJing Wang
Jing Wang
CYCuiping Yao
Cuiping Yao
ZZZhenxi Zhang
Zhenxi Zhang

19 December 2016 in Journal of Nanomaterials
Verified
Journal Article
Open access
PDF file
CYCuiping Yao
Cuiping Yao
LZLuwei Zhang
Luwei Zhang
JWJing Wang
Jing Wang
YHYulu He
Yulu He
JXJing Xin
Jing Xin
ZZZhenxi Zhang
Zhenxi Zhang
Gold nanoparticles exhibit very unique physiochemical and optical properties, which now are extensively studied in range of medical diagnostic and therapeutic applications. In particular, gold nanoparticles show promise in the advancement of cancer treatments. This review will provide insights into the four different cancer treatments such as photothermal therapy, gold nanoparticle-aided photodynamic therapy, gold nanoparticle-aided radiation therapy, and their use as drug carrier. We also discuss the mechanism of every method and the adverse effects and its limitations.

1 May 2015 in Journal of Biomedical Optics
Verified
Journal Article
Open access
PDF file
ZZZhenxi Zhang
Zhenxi Zhang
SWSijia Wang
Sijia Wang
HXHao Xu
Hao Xu
BWBo Wang
Bo Wang
CYCuiping Yao
Cuiping Yao
Since photodynamic therapy (PDT) has many advantages such as its minimally invasive procedure, low morbidity, minimum functional disturbance, good tolerance, ability to be used repeatedly at the same site, and the fact that it is largely an outpatient therapy,1,2 it is a promising treatment modality for cancers and other malignant diseases.

1 January 2009 in Journal of Biomedical Optics
Verified
Journal Article
Open access
PDF file
CYCuiping Yao
Cuiping Yao
XQXiaochao Qu
Xiaochao Qu
ZZZhenxi Zhang
Zhenxi Zhang
GHGereon Hüttmann
Gereon Hüttmann
RRRamtin Rahmanzadeh
Ramtin Rahmanzadeh
Light-absorbing micro- and nanoparticles, which are bound to cell membranes and irradiated by lasers, have been proven to create highly localized cell damage. By this method, membranes can be permeabilized1 and our previous results demonstrated the ability to introduce exogenous molecules into mammalian cells using this technique.2, 3