Phytomedicine refers to the use of naturally derived products to cure and mitigate human conditions. Natural products have the advantages of causing minimum side effects, being biocompatible, available, and economical, with a wide array of biological activities. Reports have described the use of natural products with antimicrobial and anti-inflammatory properties to treat oral conditions and promote wound healing. Moringa oleifera, known as the drumstick or horseradish tree, is believed to have medicinal properties regarding a range of medical conditions, though there is limited information on its use in oral medicine. This narrative review focuses on the use of Moringa extracts in the management of oral conditions, including oral infections, inflammatory conditions, the remineralization of hard tissues, oral wound healing, and tissue regeneration, drawing from both in vitro and in vivo studies which indicate that the potential of Moringa extracts in supporting dentin-pulp regeneration after caries or trauma is worthy of more careful consideration.

Background: Regeneration dentistry demonstrates significant challenges due to the complexity of different dental structures. This study aimed to investigate osteogenic differentiation of human pulp stem cells (hDPSCs) cultured on a 3D-printed poly lactic acid (PLA) scaffold coated with nano-hydroxyapatite (nHA) and naringin (NAR) as a model for a dental regenerative. Methods: PLA scaffolds were 3D printed into circular discs (10 × 1 mm) and coated with nHA, NAR, or both. Scaffolds were cultured with hDPTCs to identify cellular morphological changes and adhesion over incubation periods of 3, 7, and 21 days using SEM. Then, the osteogenic potential of PLA, PLA/nHA/NAR, or PLA scaffolds coated with MTA elutes (PLA/MTA scaffolds) were evaluated by measuring mineralized tissue deposition using calcium concentration assays and alizarin red staining (ARS). Also, immunofluorescence labelling of alkaline phosphatase (ALP) and dentine sialophosphoprotein (DSPP) within cultured cells were evaluated. Results: The highest cellular attachment was identified on the PLA/nHA/NAR scaffold, with morphological changes reflecting cellular differentiation. The highest calcium deposition and ARS were recognized in the PLA/nHA/NAR culture, with statistically significant difference (p < 0.05) compared to PLA/MTA. Also, ALP and DSPP markers showed statistically significantly higher (p < 0.05) immunoreactivity in cells cultured within PLA/nHA/NAR compared to PLA/MTA. Conclusions: The results confirm the osteogenic potential of PLA scaffolds coated with nHA/NAR for future animal and human investigations.

Several factors can directly damage dental pulp. Pulp healing requires controlled inflammation, which can be directed through specialized medical materials to eliminate infection and promote pulp repair. This review aimed to categorise these materials and identify their histological and molecular effects on pulp tissue or isolated cells in culture. In addition, we sought to identify which of these materials could trigger a favourable inflammatory pathway that could direct the pulpal response toward healing and regeneration. A single database (PubMed) was used, and the search strategy was based on MeSH terms. The search was conducted for articles published in English between January 2010 and December 2023, including those with histological and molecular findings. Only 33 articles met our inclusion criteria. Several conventional pulp capping agents have been shown to induce pulp healing and repair through dentine bridge formation. These materials show varying degrees of inflammation, ranging from moderate to mild, which may diminish over time. Other experimentally developed materials were also studied, either alone or in combination with conventional products; these materials demonstrated promising potential to reduce inflammation and superficial necrosis associated with conventional products. However, they still do not meet all the criteria for ideal pulp-capping materials and need further development for commercialisation. Several inflammatory pathways were also addressed in this review, along with favourable tissue responses to induce pulp regeneration. The immunomodulatory role of M2 phenotype macrophages is currently the most accepted, though the lack of standardised experimental procedures across studies hinder precise decision-making.

The use of medicinal plant preparations to clean and disinfect root canal infection is gaining popularity. The aim of this study was to evaluate the bioactive composition of leaf extracts of Moringa oleifera plants cultivated in Iraq (specifically Baghdad) and their antimicrobial activity against selected root canal pathogens for potential application in endodontic treatment. Materials and Methods. Moringa leaf extracts were prepared either through cold maceration or warm digestion techniques to perform an ethanolic or aqueous extraction, respectively. Phytochemical detection was performed before thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC) to measure flavonoids and phenolic compounds within both extracts. Then, their antimicrobial activities were investigated against Streptococcus mutans, Enterococcus faecalis, and Candida albicans through minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC), and agar well diffusion assay in comparison to NaOCl and Ca(OH)₂. Results. Phytochemical screening showed several active ingredients but with higher expression of flavonoids and phenolic compounds. Also, different types of these compounds were detected through TLC and quantified by HPLC. MIC values for ethanolic extract against Streptococcus mutans, Enterococcus faecalis, and Candida albicans were 60, 65, and 55, respectively, while for aqueous extract, MIC values were 70, 80, and 50, respectively. Aqueous extract showed a higher inhibition zone than ethanolic extract for both Streptococcus mutans and Enterococcus faecalis with a statistically significant difference ( ) for all tested materials except with NaOCl and Ca(OH)₂ in Streptococcus mutans and Enterococcus faecalis, respectively. The ethanolic extract showed a higher inhibition zone against Candida albicans, with a statistically significant difference ( ) for all tested materials. Conclusion. Ethanolic and aqueous extracts of Moringa oleifera leaves cultivated in Baghdad contain considerable quantities of phytochemicals, especially flavonoid and phenolic compounds, and demonstrated antimicrobial activities against selected endodontic pathogens. Therefore, Moringa leaf extracts could be suggested as an alternative antimicrobial material in endodontic treatment.

Background: Morphology of the root canal system is divergent and unpredictable, and rather linked to clinical complications, which directly affect the treatment outcome. This objective necessitates continuous informative update of the effective clinical and laboratory methods for identifying this anatomy, and classification systems suitable for communication and interpretation in different situations. Data: Only electronic published papers were searched within this review. Sources: “PubMed” website was the only source used to search for data by using the following keywords "root", "canal", "morphology", "classification". Study selection: 153 most relevant papers to the topic were selected, especially the original articles and review papers, from 1970 till the 28th of July 2021. Conclusions: This review divided the root canal analysis methods into two approaches; clinical and in vitro techniques. The latter has shown more precise non-subjective readings, on the other hand; the clinical methods provide direct chair side diagnosis for the clinical cases. The classification systems reviewed in the present study, started with the oldest trials that simply presented the root canal systems, according to the degree of angulation, or by coded Latin numbers or English letters. Then, the most recent systems were also presented that were persisted with continuous editions up to date. These new systems could briefly describe the root and root canal’s internal and external details in a small formulation, without confusion and in an easily communicated manner, highly recommended specially for students, teachers, and researchers