The cardiopulmonary system is made up of the heart and the lungs, with the core function of one complementing the other. The unimpeded and optimal cycling of blood between these two systems is pivotal to the overall function of the entire human body. Although the function of the cardiopulmonary system appears uncomplicated, the tissues that make up this system are undoubtedly complex. Hence, damage to this system is undesirable as its capacity to self-regenerate is quite limited. The surge in the incidence and prevalence of cardiopulmonary diseases has reached a critical state for a top-notch response as it currently tops the mortality table. Several therapies currently being utilized can only sustain chronically ailing patients for a short period while they are awaiting a possible transplant, which is also not devoid of complications. Regenerative therapeutic techniques now appear to be a potential approach to solve this conundrum posed by these poorly self-regenerating tissues. Stem cell therapy alone appears not to be sufficient to provide the desired tissue regeneration and hence the drive for biomaterials that can support its transplantation and translation, providing not only physical support to seeded cells but also chemical and physiological cues to the cells to facilitate tissue regeneration. The cardiac and pulmonary systems, although literarily seen as just being functionally and spatially cooperative, as shown by their diverse and dissimilar adult cellular and tissue composition has been proven to share some common embryological codevelopment. However, necessitating their consideration for separate review is the immense adult architectural difference in these systems. This review also looks at details on new biological and synthetic biomaterials, tissue engineering, nanotechnology, and organ decellularization for cardiopulmonary regenerative therapies.

Background: A critical unmet medical need in clinical management of colorectal cancer (CRC) pivots around lack of noninvasive and or minimally invasive techniques for early diagnosis and prognostic prediction of clinical outcomes. Because DNA methylation can capture the regulatory landscape of tumors and can be measured in body fluids, it provides unparalleled opportunities for the discovery of early diagnostic and prognostics markers predictive of clinical outcomes. Here we investigated use of DNA methylation for the discovery of potential clinically actionable diagnostic and prognostic markers for predicting survival in CRC. Methods: We analyzed DNA methylation patterns between tumor and control samples to discover signatures of CpG sites and genes associated with CRC and predictive of survival. We conducted functional analysis to identify molecular networks and signaling pathways driving clinical outcomes. Results: We discovered a signature of aberrantly methylated genes associated with CRC and a signature of thirteen (13) CpG sites predictive of survival. We discovered molecular networks and signaling pathways enriched for CpG sites likely to drive clinical outcomes. Conclusions: The investigation revealed that CpG sites can predict survival in CRC and that DNA methylation can capture the regulatory state of tumors through aberrantly methylated molecular networks and signaling pathways.

Background: the development and progression of triple-negative breast cancer (TNBC) is driven by somatic driver mutations and the tumor-immune microenvironment. To date, data on somatic mutations has not been leveraged and integrated with information on the immune microenvironment to elucidate the possible oncogenic interactions and their potential effects on clinical outcomes. Here, we investigated possible oncogenic interactions between somatic mutations and the tumor-immune microenvironment, and their correlation with patient survival in TNBC. Methods: We performed analysis combining data on 7,875 somatic mutated genes with information on 1,751 immune-modulated genes, using gene-expression data as the intermediate phenotype, and correlated the resulting information with survival. We conducted functional analysis to identify immune-modulated molecular networks and signaling pathways enriched for somatic mutations likely to drive clinical outcomes. Results: We discovered differences in somatic mutation profiles between patients who died and those who survived, and a signature of somatic mutated immune-modulated genes transcriptionally associated with TNBC, predictive of survival. In addition, we discovered immune-modulated molecular networks and signaling pathways enriched for somatic mutations. Conclusions: The investigation revealed possible oncogenic interactions between somatic mutations and the tumor-immune microenvironment in TNBC, likely to affect clinical outcomes.

BACKGROUND: To be acquainted with gastrointestinal vasculature variations is of utmost importance for establishing proper surgical management, improving safety, and decreasing the frequency of iatrogenic errors or complications arising in operational and post-operational settings. CASE REPORT: The subject of the present publication involves a unique case of an 80-year-old Caucasian male who presented with various vascular variations during routine cadaveric dissection. Key variations presented in this report include unique findings such as an abnormal trifurcation of the celiac trunk, a bifurcation of the superior mesenteric artery, and its associated branches; an unusual portocaval system communication; and various renal variations. These variations are examined in an anatomical and clinical context. We further discuss the possible embryologic and genetic mechanisms which may lead to such vascular abnormalities. CONCLUSION: Furthermore, with this report, we aim to demonstrate the strong need for adequate knowledge of vascular variations as well as the important role of pre-operative imaging in the identification of vascular variations and the elimination of iatrogenic errors during surgical procedures.

Title: Feeding habits and its impact on concentration and attentiveness among medical students in Dominica. Background: Food is essential for good health. Aside other benefits, it has been acknowledged that the status of the learning process is directly proportional to good nutrition. However, studies have shown that majority of medical student do not participate in a healthy dietary plan, because of time and workload. As a result, it is suspected that some medical students may have a poor learning process. This study thus tries to correlate food and academic performance among medical students in Dominica. Methods and Findings: A total of 182 students participated. Self-administered questionnaires were distributed and analysis done by Stata IC 13. Amongst the group studied, 169 (92.35%) acknowledged to skipping meals, while 111 (62.01%) preferred junk food to healthy diets while studying. Majority of students with poor intake of balanced diet could only spend 3-4 hrs studying. Also, students who had regular breakfast testified to be able to stay more than 6 hrs studying. Conclusion: This study supported the fact that food plays a vital role in the learning process of students. In addition to identifying the correlate, the study also recognized the need for proper student awareness on food choices.

In recent years, there have been increasing efforts to further our understanding of the toxic potential or otherwise of nanomaterials. These efforts are laudable and welcome, however, we cannot afford to be oblivious to some factors that could influence our interpretation of the nanotoxicity data and to a larger extent our ability to accurately predict the biological effects of these materials. Among these factors are the choice of test model, choice of assay, and proper characterization of nanoparticles and/or nanomaterials to name a few. In the current chapter, we will be presenting an overview of in vivo nanotoxicity test models, and highlighting, comparing, and discussing the consistency and otherwise of available nanotoxicity reports.