• Fruit juices (FTs) are important nutritional part of daily life. • Demand for FJs processing with a certain safety standards urges nonthermal technologies (NNTs). • NTTs can causes inactivate enzyme by conformational alteration. • Food matrix, processing intensity, properties of enzymes and proteins present in FJs may affect NNTs efficiency. • Combination of NNTs may cause synergistic enzyme inactivation effect. • NNTs employment potentially ensures to maintain the FJs quality.

A novel rapid analytical method for the determination of four non-steroidal anti-inflammatory drugs (NSAIDs) – nabumetone, ibuprofen, naproxen and diclofenac – in tap water and drinks is presented. The method is based on ultrasound-assisted ionic liquid dispersive liquid–liquid microextraction (US-IL-DLLME) followed by ultra-high performance supercritical fluid chromatography (UHPSFC) coupled to a photo-diode array detector (PDA). The ionic liquid 1-octyl-3-methylimidazolium hexafluorophosphate ([C₈MIM][PF₆]) and methanol were used as the extraction and dispersion solvents for the DLLME procedure other than using a toxic chlorinated solvent. Plackett–Burman and Box–Behnken designs were applied as the experimental design strategies to screen and optimize the experimental variables such as the volume of the ionic liquid, the volume of the disperser solvent, sample pH, ionic strength, ultrasonication time and centrifugation time which affected the extraction efficiency. Separation conditions of UHPSFC, such as column screening, modifiers, column temperature, back pressure and flow rate, were also optimized in this study. 4 NSAIDs were simultaneously separated and determined in 2.1 minutes. The optimized US-IL-DLLME-UHPSFC-PDA method showed good enrichment factors (126–132) and recoveries (81.37–107.47%) for the rapid extraction of nabumetone, ibuprofen, naproxen and diclofenac in tap water and drinks. The method's limits of detection for nabumetone, ibuprofen, naproxen and diclofenac were 1.56, 7.69, 0.62, and 7.37 ng mL⁻¹ with excellent linearity (R > 0.9957).