Rice (Oryza sativa L.) is cultivated in a wide range of climatic conditions, thereby inducing great variations in the rice growth, yield and quality. However, the comprehensive effects of temperature and solar radiation under different ecological regions on the rice growth, yield and quality are not well understood, especially in a middle rice cropping system. The rice growth, yield- and quality-related traits were investigated under different ecological regions. Among different areas, the days before the heading stage and after the heading stage of six cultivars ranged from 80 to 120 and from 30 to 50. The gaps of the grain yield, head rice rate, chalky grain rate and chalkiness level were about 1.2–52.4%, 1.0–3.0%, 2.7–12.7% and 0.3–4.5%, respectively. This study demonstrated that in these regions, temperature is a limiting factor compared with radiation. Moreover, the rice growth, yield and quality were closely associated with daily air (DT), maximum (MaT), minimum (MiT) and effective accumulated temperatures (EAT). An excellent rice growth, a high grain yield and an excellent quality could be achieved if the EAT was higher than 1592 °C·d and the MiT was lower than 23.1 °C before the heading stage, and if the DT, MiT and MaT were lower than 25.7 °C, 22.0 °C and 30 °C after the heading stage, respectively. These findings served as an important reference for optimizing cultivar selection for a specific area and determining suitable areas for a certain variety.

In this review, the significance of ratoon rice was introduced, and the research status and development trends of ratoon rice were also summarized. It is pointed out that mechanically harvested ratoon rice is the developing direction of future ratoon rice. On this basis, we analyzed the relationship between the yield of ratoon rice and many factors, such as variety characteristics, sowing date, water control, fertilizer, and many others. It is important to construct a comprehensive and practical evaluation system for rice regeneration that can provide a basis for high-yield cultivation of machine-harvested ratoon rice. At the same time, it is suggested that combining high-yield cultivation with the green ecological efficiency of rice can achieve better production and improve the quality of rice. Finally, some problems with ratoon rice development were put forward. An in-depth study on the rhizosphere biology and regulation techniques of ratoon rice and the effective ecological compensation mechanism increased the capacity and quality of ratoon rice. Further, the functioning of such research can enhance the planting area for ratoon rice and improve food security.

Mechanized production plays an important role in fulfilling food security demands during the period of labor shortage. Despite its benefits, the mechanical transplanted method (MET) has not been widely adopted due to a lack of awareness. Thus, this study aimed to evaluate the effect of the MET, the manual transplanted method (MAT), and the directed seeded method (DS) on rice yield and quality in farmers’ fields. A two-years field experiment (2016 and 2017) and a one-year survey (2020) were conducted to compare rice yield and quality among the MAT, MET, and DS methods. MET exhibited a higher-yielding population, increased biomass production, enhanced yield, and improved grain quality, compared with MAT. Moreover, japonica rice in MET (MET-JR) produced the maximum yield, 0.6 t hm⁻² to 3.1 t hm⁻² higher than in other treatments. However, japonica rice showed a poorer appearance quality than indica rice, as well as large panicle size (grains number per panicle ≥ 190); hybrid indica rice (HIR) also presented a high yield with poor appearance quality. These results confirmed that the application of MET could be useful in attaining high panicles per m², high biomass production, high rice yield, and considerably improved rice quality in farmers’ fields under labor shortage circumstances. Furthermore, it is also imperative to consider balancing the yield and quality of japonica rice and large panicle HIR and employing MET at a broader scale in China, as well as other developing countries having rice–based cropping systems.

Grain chalkiness adversely affects rice quality, and the positional variation of grain chalkiness within a rice panicle presents a substantial obstacle to quality improvement in China. However, the molecular mechanism underlying this variation is unclear. This study conducted a genetic and physiological analysis of grains situated at distinct positions (upper, middle, and bottom primary branches of the rice panicle, denoted as Y1, Y2, and Y3) within a rice panicle using the Yangdao 6 variety. The results indicated that the percentage of chalky grains (PCG) in Y1 was the highest, i.e., 17.12% and 52.18% higher than that of Y2 and Y3, respectively. Y2 exhibited the highest degree of grain chalkiness (DGC), attributable to its greater area of endosperm chalkiness (AEC) than the others. Y3 demonstrated the lowest PCG and DGC. Additionally, Y1 and Y2 were characterized by lower amylose and protein contents, as well as looser starch granule morphology, in comparison to Y3. Compared with Y3, both the average and maximum filling rates of Y1 and Y2 increased markedly; however, the active filling duration was notably reduced by 7.10 d and 5.56 d, respectively. The analysis of genomic expression levels indicated an enrichment of starch and sucrose metabolism in Y1-vs.-Y2, Y2-vs.-Y3, and Y1-vs.-Y3, with 7 genes (5 up-regulated and 2 down-regulated), 53 genes (12 up-regulated and 41 down-regulated), and 12 genes (2 up-regulated and 10 down-regulated) in the Y1-vs.-Y2, Y2-vs.-Y3, and Y1-vs.-Y3. The majority of these genes were down-regulated, linking metabolic activity to grain filling and contributing to the occurrence of grain chalkiness in rice panicles. In conclusion, the metabolic processes associated with sucrose and starch play a crucial role in regulating grain filling and the formation of chalkiness in rice.

Grain protein is a critical quality attribute of rice that influences consumer preferences. However, the spatial variation in protein accumulation within a rice panicle remains poorly understood. This study investigated the dynamics of protein accumulation, including protein components and protein synthesis-related enzymes and genes, among grains located at the top, middle, and bottom primary rachises of a rice panicle during the grain filling. The results revealed significant variations in protein compositions across different rachis positions. The contents of albumin, globulin, prolamin, glutelin, and total protein contents exhibited fluctuations during grain filling. Notably, the grain position had a significant effect on glutelin content, with grains at the bottom primary rachis consistently having higher glutelin level than those at the top and middle rachises, except 17 days after flowering (DAF). A similar trend was observed for total protein content. Grains at the bottom rachis demonstrated dominance in the rate of protein accumulation, initiating rapid accumulation 2.0 d later and 2.2 d earlier than grains at the top and middle rachises, respectively. Furthermore, the duration of active protein accumulation was 1.9 d and 3.4 d shorter for grains at the bottom rachis compared to those at the top and middle rachises, respectively. This phenomenon was attributed to alterations in enzymatic activities. Specifically, the activities of glutamine synthetase (GS), glutamate synthase (GOGAT), glutamate pyruvate transaminase (GPT), and glutamic oxalo-acetic transaminase (GOT) in grains located at the basal rachis exhibited a marked increase from 8 DAF to 17 DAF. These activities were significantly elevated compared to those observed in grains at the top and middle rachis, although they experienced a subsequent sharp decline. The glutelin content and enzymatic activities demonstrated a strong correlation, either positive or negative, at 11 DAF and 20 DAF. These findings suggest that the positional changes of grain protein were closely associated with nitrogen assimilation and glutelin accumulation during the rice grain filling process.