Background and Aim: Secondary bioactive compounds of medicinal plants exert anti-inflammatory, antimicrobial, antioxidant, and metabolism-modulating effects. This study aimed to investigate the effect of feeding 4-hexylresorcinol, as well as its combinations with gamma-octalactone and 7-hydroxycoumarin, on the digestibility of dietary nutrients, weight gain, and quality characteristics of the meat and liver of Arbor Acres broiler chickens. Materials and Methods: The following feeding scheme was applied on the chickens: Control, basal diet (BD); I experimental, BD + 4-hexylresorcinol at 0.5 mg/kg of live weight per day; II experimental, BD + 4-hexylresorcinol + gamma-octalactone at 0.4 mg/kg of live weight per day; III experimental, BD + 4-hexylresorcinol + 7-hydroxycoumarin at 0.1 and 0.15 mg/kg of live weight per day; and IV experimental, BD + 4-hexylresorcinol + gamma-octalactone + 7-hydroxycoumarin at 0.05, 0.15, and 0.01 mg/kg of live weight per day. Results: Chickens in I, II, and IV experimental groups at the age of 35 days showed superior live weight than chickens in the control group. Supplementation with all the tested additives, except the combination 4-hexylresorcinol + 7-hydroxycoumarin, significantly increased the digestibility coefficients of dietary nutrients. Supplementation with the combinations 4-hexylresorcinol + gamma-octalactone and 4-hexylresorcinol + gamma-octalactone + 7-hydroxycoumarin significantly increased the amount of fat in the pectoral muscles. However, the mass fraction of fat in the thigh muscles of broiler chickens decreased in II, III, and IV experimental groups. The pectoral muscles of broiler chickens in experimental Group IV contained small amounts of lysine, tyrosine, histidine, leucine–isoleucine, methionine, valine, proline, threonine, serine, alanine, and glycine. Supplementation with pure 4-hexylresorcinol significantly reduced the levels of lysine, phenylalanine, histidine, leucine–isoleucine, methionine, valine, proline, threonine, and alanine in the thigh muscles. However, supplementation with pure 4-hexylresorcinol significantly increased the concentrations of P, Fe, Se, Zn, and B and decreased the concentrations of I, Ni, V, Al, and Pb in the pectoral muscles. Supplementation with the combination 4-hexylresorcinol + gamma-octalactone + 7-hydroxycoumarin resulted in the accumulation of Ca, Co, Fe, Mn, Se, Zn, and Li and a decrease in the concentrations of K, Mg, and V. Conclusion: Supplementation with all the tested additives, except the combination 4-hexylresorcinol + 7-hydroxycoumarin, exerted a positive effect on the indicators of live weight gain and dietary nutrient digestibility in broiler chickens. Supplementation with the combinations 4-hexylresorcinol + gamma-octalactone and 4-hexylresorcinol + gamma-octalactone + 7-hydroxycoumarin increased the amount of fat in the pectoral muscles but decreased it in the thigh muscles. Supplementation with all the tested additives decreased the concentrations of I in the pectoral muscles and Zn in the thigh muscles in all the experimental groups compared with those in the control group.

Background and Aim: Phytobiotics are a safe alternative to feed antibiotics in industrial poultry farming that increases productivity by stimulating various digestive enzymes to reduce the number of pathogenic microorganisms in the intestines and improve antioxidant status and immune response. This study aimed to evaluate the effect of vanillic acid in its pure form and in combination with gamma lactone on weight growth and the physiological parameters of broiler chickens. Materials and Methods: The studies were performed on 120 Arbor Acres broiler chickens (7 days old) that were randomly divided into four groups with five replicates (cages) and six birds per replicate. The control group was fed the basal diet (BD) only. Group I was fed the BD + gamma lactone (average dose 0.07 mL/animal/day). Group II was fed the BD + vanillic acid (average dose 0.07 mL/animal/day). Group III was fed the BD + gamma lactone + vanillic acid in a 1:1 ratio (average dose 0.07 mL/body/day). Live weight of broiler chickens for all group was assessed at the end of each period (age of 7, 14, 21, 28, 35, 42 days). At the end of the experiment (on day 42), 10 broilers with an average live weight were selected for blood sampling from the axillary vein. Results: The results showed a growth-promoting effect of vanillic acid when fed with a diet free of synthetic antioxidants. Groups I and II had increased live weights throughout the entire experiment and a significant increase at the end of the experiment (8.2%–8.5%; p ≤ 0.05) compared to the control group. Toxic effects were not found in the biochemical blood serum analyses of Groups II and III. The metabolic processes in the experimental groups were intensified, especially in the enzyme associated with amino acid metabolism (gamma-glutamyl transferase) in Groups I and III (p ≤ 0.05). Vanillic acid, whether fed alone or in combination with gamma lactone, exhibited high antioxidant activities, protected cells from oxidative damage by inducing total antioxidant, catalase, and superoxide dismutase activities (p ≤ 0.05), and reduced the level of malondialdehyde (p ≤ 0.05) measured. No significant changes in the morphological blood parameters were found. Conclusion: The use of vanillic acid alone and in combination with gamma lactone increases the digestive enzyme activities in blood plasma, increases body weight, and has a positive effect on lipid metabolism and the antioxidant status of broiler chickens. These findings indicate the significant potential use of vanillic acid and gamma lactone in poultry due to their antioxidant properties. Keywords: antioxidant status, Arbor Acres cross, blood, broiler chickens, feeding, gamma lactone, productivity, vanillic acid.

Annotation. The study was performed on clinically healthy cows of Holstein breed during the period of milking in the CJSC "Gatchina" of the Leningrad region (Russia). Animals (n = 60) were divided into groups depending on the severity of "loaded metabolism" estimated by lead concentration in wool: up to the 25th centile, within the 25th to 75th centile, above the 75th centile. The concentration of lead in wool was determined by atomic emission and mass spectrometry (AES-ICP and MS-ICP). It was found that about the increase in lead content from the minimum to the maximum in centile intervals from 25-75 to> 75, the level of malondialdehyde (Total-MDA) in the blood increased, which was recorded against the background of a decrease in milk productivity. A significant negative correlation was observed between the concentration of lead in wool and the yield of milk fat, protein, dry matter, and corrected for 1% milk fat.