Aim: Through the use of CBCT images, many unidentified features of impacted canines can be easily resolved. The potential collision of impacted maxillary canines and adjacent teeth could lead to root resorption (RR). The aim of this study is to examine the prevalence, location and severity of RR on adjacent teeth caused by impacted maxillary canines and the association between the adjacent teeth and the features of maxillary impacted canines on CBCT. Methods: This study examined 89 subjects with 108 maxillary impacted canines, having had no previous orthodontic treatment (mean age: 18.3 ± 4.1 years). The following impacted-canine-related parameters were analyzed on the CBCT images: location; RR levels on adjacent teeth; occlusal line and midline distances of impacted canines; and the angulations of impacted canines to the midline, lateral incisor and occlusal line. Logistic regression was used to evaluate the association between RR and the measured parameters on CBCT. Results: In this study, we found that the majority of our patients with impacted maxillary canines were female (62.5%). Of the total 108 maxillary impacted canines, 60.2% resorbed the adjacent teeth of the affected quadrants. Lateral incisors were the most affected (34.3%). The mean age of subjects with RR was 16.7 ± 3.5 years. The frequency of RR was statistically significantly higher in female subjects (40.4%). Slight RR was the most frequent (30.5%) and the highest incidence noted at the apical third of the root (29.6%). Regarding the impacted maxillary canine angulation to the midline and adjacent tooth, higher values of angulation caused severe forms of RR (p < 0.05). Conclusion: The sensitivity of CBCT allows for the accurate diagnosis of the location and the degree of RR, alongside the angulation and distance of impacted canines to adjacent teeth. The association between the linear and angular features of the impacted maxillary canines and RR was confirmed.

Background/Aim. Proinflammatory cytokines can act like endogenous pyrogen interleukin 1 (IL-1), interleukin 6 (IL-6) and tumour necrosis factor alpha (TNF ?) which regulate the synthesis of secondary mediators and other proinflammatory cytokines through macrophages and mesenchymal cells. They stimulate acute-phase proteins and attract inflammatory cells. The aim of this study was to determine interleukin 1-? (IL-1 ?) concentrations in chronically inflamed and healthy dental pulps. Methods. A total of 41 pulps (19 from patients with pulpitis chronic causa and 22 from patients with pulpatis chronic aperta), divided into two groups, were obtained from teeth with chronic pulp inflammation. The control group consisted of 12 teeth with healthy pulp. After extirpation, pulp samples were immediately placed in sterile Eppendorf tubes and frozen. After that, homogenisation was performed by a Teflon? pestle in ice-cold phosphate buffer solution at pH 7.4 whose volume was adjusted according to the weight of tissue. The supernatant was then frozen at -70?C until the performance of appropriate biochemical analyses. Cytokine IL-1 ? value was determined by a commercial enzyme- linked immunosorbent assay (ELISA test). We applied the high sensitivity system technique, which may register low levels of cytokines, ranging from 0.125 to 8.0 pg/mL for IL-1 ?. Results. By comparing the mean value of IL-1?, in the pulps we can see a statistically significant difference (p < 0.01) among them. The highest value of IL-1 ? was in the subjects with pulpitis chronica clausa and it was 6.21 ? 2.70 pg/mL. Conclusion. Proinflammatory cytokine IL-1 ? is present in detectable quantities in the pulp tissue of all vital pulps. Its highest concentrations were found in the sample group with pulpitis chronica clausa.

Background/Aim. Treatment of periodontitis undergoes several treatment phases. Non-surgical periodontal treatment (NSPT) represents the basic treatment stage, and it is applied to all the patients undergoing periodontal treatment. Adjunctive antimicrobial photodynamic therapy (aPDT) is one of several contemporary and relatively new possibilities with a role to inactivate microorganisms responsible for the occurrence and progression of the disease. The aim of this study was to comparatively analyze the clinical and microbiological effects of the NSPT alone, as well as combined with aPDT. Methods. A split-mouth method design was used in a prospective randomized controlled trial. The following clinical parameters were registered and monitored: plaque index (PI), bleeding on probing, probing depth (PD), and clinical attachment level (CAL). The presence of microorganisms Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Treponema denticola was detected by the polymerase chain reaction (PCR) method. Samples were tested before the therapy, as well as three months after the therapy. Therapeutic modalities of NSPT and NSPT with adjunct aPDT were examined. Results. All of the analyzed clinical parameters proved statistically significant improvement after the application of both treatment modalities (p < 0.001). Microbiological analyses showed that the total number of microorganisms was statistically significantly lower after the application of both methods (p < 0.001). Following the treatment, there was a statistically significantly reduced number of microorganisms Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Treponema denticola (p < 0.001). NSPT combined with aPDT led to a statistically significant improvement of both clinical parameters and microbiological status compared to NSPT applied on its own. Conclusion. The study showed improvement of all clinical indicators after the application of both treatment modalities. The total number of microorganisms was reduced as well as the number of specific microorganisms. Combining aPDT with NSPT led to a statistically significantly higher reduction in the number of microorganisms compared to NSPT alone.

Background/Aim. Many authors find that impacted maxillary canines is associated with missing and peg-shaped lateral incisor. The aim of this study was to examine the prevalence of peg-shaped and missing lateral incisor in subjects with impacted maxillary canines, and compare the size of maxillary lateral incisor on the side with palatally impacted canines and on the opposite side of the jaw where there is no impaction. Methods. The study included 64 patients with 80 impacted maxillary canines (23 males and 41 females, mean age 16.3). For each maxillary unerupted canine, precisely correct localization and classification into groups was done. We analyzed the morphology of the lateral incisor (normal, atypical) and frequency of missing of lateral maxillary incisors with canine impaction. Then, from the mentioned examinees sample with the maxillary canine teeth, a subgroup was formed. The criteria for selection were those with unilateral palatally impacted canines (33 subjects, 22 females and 11 males, mean age 17.8 years). The linear variables of the maxillary lateral incisor were measured by using digital measurements tools. The t-test was used to test the differences between the groups. Results. Normal morphology of the lateral incisors was found in 72% of the subjects with the impacted canines, 11.2% of the subjects had the peg-shaped lateral incisors, 6% had a bilateral and 4% had unilateral deficiency of lateral incisors. In the subgroup of the patients with unilateral palatal impaction, the middle value of the length of the lateral incisors was 1.9 mm shorter and the middle value of the width of the lateral incisors was smaller by 0.9 mm when comparing to the control group. Conclusion. The frequency of the deficiency of lateral incisors was statistically significantly higher in the group with palatal canine impaction. The maxillary lateral incisors on the side with palatally impacted canines were smaller than those on the side where there was no impaction.

Background / Aim. In the planning of posterior teeth extraction it is necessary to determine their position and correlation with the surrounding anatomical structures. The aim of this study was to perform CBCT analysis in order to evaluate predictive value of specific OPT radiographic signs in determining the proximity of posterior upper teeth to the maxillary sinus and lower teeth to mandibular canal. Methods. In a prospective study, 460 cases out of 423 patients were analyzed. Seven OPT radiographic signs were assessed to determine the correlation of the third molars to the mandibular canal, while five radiographic signs were followed to determine the correlation of the maxillary sinus floor to the upper posterior teeth. For each OPT radiographic sign a precise analysis of the axial slices of the CBCT was performed. Results. Interruption of the white line is a radiographic sign showing statistically significant interruption of the integrity of the mandibular canal on the CBCT axial slices (p=0.002). Also, diversion of the inferior alveolar canal at the axial sections shows statistically significant interruption of the continuity of the mandibular canal (p=0.003). Radiographic sign darkening of the root showed superimposition of the anatomical structures, a close relationship with the tooth but often with preserved mandibular canal integrity (p<0.001). Absence of lamina dura, interruption of the maxillary sinus floor's cortex and projection of the root apices in the sinus cavity are radiographic signs that were statistically the most commonly associated with interruption of maxillary sinus integrity on CBCT axial slices (p<0.001). Conclusion. The results of our study indicate that the presence of certain radiographic signs on the OPT may have predictive significance. The exact relationship between anatomical structures and posterior teeth cannot be accurately estimated on OPT. The precise position of the molars and correlation to the mandibular canal and the maxillary sinus can be accurately determined by CBCT imaging.

Correlation of fillings on the tooth and changes in the tissues having direct contact with the tooth aroused keen interest amongst many professionals. Irregularly shaped fillings can damage the periodontal tissues. The primary indicator of the validity of the dental work is its ability to ensure the preservation of normal physiological status of the tissue, primarily gingival, alveolus and periodontum. The main purpose of this paper is to make histological assessments of the tissue of the interdental region before and after correction of irregular filling in experimental animals. The research was conseived as a histiological study with experimental animals ( rabbits). The tested teeth were divided into two groups: a) the experimental group consisted of teeth with irregular fills, and b)the control group consisted of teeth with regular fillings and healthy teeth. The experiment carried out under general anesthesia. The experiment was being monitored over 12-month period. Having been expired the first part of the experiment , after 6-month period, in experimental animals, it was carried out the second part of the one consisting of the correction of irregular fillings. The second part of thhe experiment was closely monitored a month, three and six months after the correction of irregular filings. After sacrificing the animals, tissue was prepared for histological analysis. Histological analysis showed changes in the periodontal tissues prior to correction of irregular fillings and significant improvementof the tissue of the interdental region after correction of the same ones. The pathological changes occuring in the periodontal tissues are not irreversible since after correction of irregular filligs are being lost mainly.