Under extreme drought and climate change, golden pear trees have experienced problems such as yield reduction, dryness and death. This suggests that we know very little about the mechanisms regulating pear tree growth, assuming that meteorological factors positively influence plant sap flow. Based on this, we used the heat ratio method to monitor the sap flow of pear trees from June to December 2020, and recorded the changes in various environmental factors. The results showed that: (1) Sap flow velocity has obvious radial variability in tree sections; the sap flow velocity during the day was significantly higher than that at night (p < 0.05) and was higher in the growing season than in the non-growing season. (2) All environmental factors, except relative humidity and precipitation, were positively correlated with sap flow, vapor pressure deficit and photosynthetically active radiation, which are the key factors affecting daytime flow, and vapor pressure deficit and plant water potential are the key factors affecting nighttime flow. The linear regression results also showed that the daytime sap flow had a significant positive effect on the nighttime sap flow (p < 0.05). (3) The contribution of night flow to total daily flow varied from 17.3% to 50.7%, and most of the non-growing season values were above 40%. The results show that nighttime sap flow accounts for a significant portion of the pear tree’s water budget. Continuous irrigation during fruit enlargement and non-growing seasons will increase fruit yield and maintain plant sap flow activity to avoid death due to drought.

Subtropical forests in southwestern karst areas are the top priority for ecosystem restoration, as studying the water absorption strategies of the major plants in these regions is crucial to determining the species distribution and coexistences within these seasonal subtropical forests, which will help us to cope with the forest ecosystem crisis under future climate change. We used the stable isotope ratios (δD and δ¹⁸O) of tree xylem and soil water to assess the seasonal changes in the water use patterns and hydrological niche separations of four dominant tree species in seasonal subtropical forests in southwestern karst areas. The results showed that the soil water’s isotopic composition varied gradiently in the vertical direction and that the variation of the soil water’s isotopic composition was greater in the shallow layer than in its depths. Juglans regia (HT) mainly depended on soil water at a depth of 30–60 cm (41.8 ± 6.86%) and fissure water (32.5 ± 4.21%), while Zanthoxylum bungeanum Maxim (HJ) and Eriobotrya japonica Lindl (PP) had the same water use pattern. In the dry season, HT competed with HJ and PP for water resources, and in the rainy season, HJ and PP competed with Lonicera japonica (JYH), while HJ competed with PP all the time. JYH and HT were in a separate state of hydrologic niche and they did not pose a threat to each other. Coexisting trees are largely separated along a single hydrological niche axis that is defined by their differences in root depth, which are closely related to tree size. Our results support the theory of hydrological niche isolation and its potential responses in relation to drought resistance. This study provides a method for determining more efficient plant combinations within karst forest vegetation habitats and its results will have important implications for ecosystem vegetation restoration.