• Ozone disinfected seed-borne bacteria and fungi from seeds and ornamental bulbs. • Gaseous ozone showed better performance than ozonated water. • Pre-soaking the seeds in water increased the efficacy of ozone sterilization. • Sterilization success of ozone depended on the type of the seed, pathogen, and treatment conditions.

Trihoderma citrinoviride protects plants from diseases by functioning as antagonists of many pathogenic fungi or by triggering the antioxidant defense system in plants. In the present study, to uncover the possible alleviative role of Trichoderma against salinity and Rhizoctonia solani infection, strawberry plants were pretreated Trichoderma citrinoviride and then subjected to salinity, R. solani and combined salinity and R. solani. The effect of T. citrinoviride on the alleviation of the effects of salt stress and Rhizoctonia solani infection was investigated by analysing leaf dry weight, PSII efficiency, and the activity of some antioxidant enzymes in the leaves of strawberry plants. T. citrinoviride improved competitive capability against salinity and R. solani infection. It showed 79% inhibition of the growth of pathogen R. solani. T. citrinoviride reduced 63% of the severity of disease in the leaves. Trichoderma pretreatment maximized plant dry weight. The T. citrinoviride-pretreated plants showed higher levels of PSII efficiency (Fv/Fm). Decreased lipid peroxidation and H₂O₂ accumulation compared to untreated seedlings under salt stress and R. solani infection was observed. Trichoderma-pretreated and –untreated plants respond differently to salt stress and R. solani infection by means of antioxidant defense. As compared to untreated seedlings, treated seedlings showed significantly lower activities of antioxidant enzymes, superoxide dismutase (SOD), peroxidase (POX), cell wall peroxidase (CWPOX) under salt stress and R. solani infection, indicating that treated seedlings might sense lower stress as compared to untreated seedlings. The study reports the effective adaptive strategy and potential of T. citrinoviride in alleviating the negative impact of salt stress and R. solani infection in strawberry.

Tr en T arımsal üretimde çevre dostu üretim tekniklerine uyarak sağlıklı ürün eldesi son yılların başlıca konusu haline gelmiştir. Bitkisel üretim sürecinde sağlıklı üretim materyali temini tarımsal üretimin en önemli ön koşulları arasında yer almaktadır. Ozon yüksek oksidasyon yeteneği ile çok kuvvetli bir dezenfektandır. Bu özelliği sayesinde birçok sektörde genel dezenfektan olarak ve farklı amaçlarla kullanılmaktadır.  Uygulama öncelikle 100-150 mg O₃/Nm³ ve 1 saat muamele olarak şekillenmiştir. Söz konusu doz ve sürede ortama uygulanan ozon gazı mevcut mikrobiyolojik yükü tümüyle eradike etmiştir. Ozon gazının 75 ve 85 mg O₃/Nm³ dozlarının 1 saat uygulanması ile de ortam atmosferi ve yüzeylerde mevcut mikrobiyolojik kontrol altına alınmıştır. Artan ozon dozu ile mikrobiyolojik yük arasında kuvvetli negatif ilişki belirlenmiştir. Benzer etki metal yüzeylere suni olarak bulaştırılan Fusarium oxysporum f. sp. lycopersici propagüllerinin dezenfeksiyonunda izlenmiştir. I n agricultural production, it has become a main topic in recent years to obtain healthy crops in accordance with environmental friendly production techniques. The most important prerequisite for agricultural production is the provide of healthy plant production material in process. Ozone is a very strong disinfectant with its high oxidation ability. With this feature, it is used as general disinfectant in many sectors and for different purposes. In this study, the possibilities of using ozone for the general disinfection of indoor environments of plant nursery facilities which are provide material to open field and greenhouses were investigated. The research has been carried out by applying high-dose ozone in gas forming for a short time in Indoor with intensive microbiological load. The treatment at 100-150 mg O₃/Nm³ and one hour has completely eradicated the microbiological loads present. The microbiological loads on the cabin atmosphere and surfaces was also controlled by applying ozone doses of 75 and 85 mg O₃ / Nm³ for 1 hour. There was a strong negative correlation between increased ozone dose and microbiological load. A similar effect was also determined on the disinfection process of artificially inoculated with Fusarium oxysporum f. sp. lycopersici propagules.