The present study reports mosquito larvicidal potential of green synthesized silver nanoparticles by using Annona glabra leaves (An-AgNPs). Synthesized An-AgNPs were characterized by Ultraviolet-Visible spectroscopy (UV-VIS), Scanning Electron Microscopy (SEM), Dynamic Light Scattering (DLS) technique and Fourier transform infrared spectroscopy (FTIR). Colur change from pale yellow to brick red of the plant extract and AgNO3 solution indicated the formation of An-AgNPs initially. Surface Plasmon Resonance (SPR) band at 435 nm in the UV-Vis confirmed the formation of An-AgNPs. SEM images showed that An-AgNPs were spherical in shape. FTIR proved that An-AgNPs were functionalized with biomolecules in A. glabra leaves. Based on DLS analysis the average size range of synthesized An-AgNPs was determine to be 10–100 nm and 100–1000 nm.

Background: The lack of information on behavioural patterns ofAedes aegyptiandAedes albopictushas become a significant limitation in vector control and disease management programmes. Therefore, the current study was focused on determining some bionomics aspects: breeding, resting, host-seeking and feeding preferences ofAe. aegyptiandAe. albopictusin Sri Lanka.Methods: Larval and adult surveys were conducted from April 2017 to April 2019 monthly in six selected Medical Officer of Health (MOH) areas in Gampaha Distinct, Western province, Sri Lanka, representing urban, suburban and rural settings. Resting preferences of adult mosquitoes were observed from indoor and outdoor places using a Prockopack aspirator. The information on resting height, surface, material and locality was recorded. Human-baited double-net traps were used to determine the host-seeking time ofAedesmosquitoes. Statistical differences in the spatial distribution of mosquitoes in selected MOH areas and prevalence of vectors were analysed using general linear model (GLM). A chi-square test was used to analyse the resting behaviour.Results: Total of 19,835 potential breeding sites were examined at 13,563 premises, and 18.5% (n = 1856) were positive forAedeslarvae. Distribution ofAe. aegyptiandAe. albopictuswas statistically significant at species level (df = 1;F = 137.134;P < 0.05 GLM) and study setting (df = 2;F = 8.125;P < 0.05).Aedes aegyptibreeding was found mainly in temporary removals (18.8%;n = 34), discarded non-degradables (12.15%;n = 22) and tyres (9.95%;n = 18). Natural (14.7%;n = 246) and temporary removals (13.6%;n = 227) and discarded non-reusable items were the key ovipositing sites forAe. albopictus. In the adult mosquito survey, the majority was comprised ofAe. albopictus(54.5%;n = 999), which denoted exophilic nature (90.8%;n = 758), and 45.5% (n = 835) represented byAe. aegyptimosquitoes who were mainly endophilic (84.3%;n = 842).Aedes aegyptirested on cloth hangings and curtains, followed by the furniture, whileAedes albopictuswas predominant in outdoor vegetation. In both vectors, biting patterns denoted a typical diurnal pattern with two peaks of host-seeking and biting activity in the morning and afternoon.Conclusions: The majority (80%) of the larval habitats were artificial containers. The use of larvicides for vector control as the prominent measure is questionable since applying these chemicals may target only 20% of the total breeding grounds, which are permanent. The resting places of adult mosquitoes are mainly indoors. Therefore, using thermal space spraying of insecticide may not be appropriate, and indoor residual spraying is recommended as a suitable intervention to target adult mosquitoes. This study warrants a holistic vector control approach for all medically important mosquitoes and insects, ensuring the rational use of finance and resources.Graphical

Immature mosquitoes are aquatic, and their distribution, abundance, and individual fitness in a particular breeding habitat are known to be dependent on mainly three factors: biotic factors, abiotic factors, and their interaction between each other and with other associated taxa. Mosquito breeding habitats harbor a diversified naturally occurring microbiota assemblage, and the biota have different types of interactions with mosquito larvae in those habitats. Those interactions may include parasitism, pathogenism, predation, and competition which cause the mortality of larvae, natural reduction of larval abundance, or alterations in their growth. Many microbiota species serve as food items for mosquito larvae, and there are also some indigestible or toxic phytoplanktons to larvae. However, when there is coexistence or mutualism of different mosquito species along with associated microbiota, they form a community sharing the habitat requirements. With the available literature, it is evident that the abundance of mosquito larvae is related to the densities of associated microbiota and their composition in that particular breeding habitat. Potential antagonist microbiota which are naturally occurring in mosquito breeding habitats could be used in integrated vector control approaches, and this method rises as an ecofriendly approach in controlling larvae in natural habitats themselves. To date, this aspect has received less attention; only a limited number of species of microbiota inhabiting mosquito breeding habitats have been recorded, and detailed studies on microbiota assemblage in relation to diverse vector mosquito breeding habitats and their association with mosquito larvae are few. Therefore, future studies on this important ecological aspect are encouraged. Such studies may help to identify field characteristic agents that can serve as mosquito controlling candidates in their natural habitats themselves.

Background: The midgut microbiota of mosquitoes maintain basal immune activity and immune priming. In recent years, scientists have focused on the use of microbial communities for vector control interventions. In the present study, the midgut bacteria of larvae and adults of Aedes aegypti and Ae. albopictus were assessed using both field-collected and laboratory-reared mosquitoes from Sri Lanka. Methods: Adults and larvae of Ae. aegypti and Ae. albopictus were collected from three selected areas in Gampaha Medical Officer of Health area, Gampaha District, Western Province, Sri Lanka. Bacterial colonies isolated from mosquito midgut dissections were identified by PCR amplification and sequencing of partial 16S rRNA gene fragments. Results: Adults and larvae of Ae. aegypti and Ae. albopictus harbored 25 bacterial species. Bacillus endophyticus and Pantoea dispersa were found more frequently in field-collected Ae. aegypti and Ae. albopictus adults, respectively. The midgut bacteria of Ae. aegypti and Ae. albopictus adults (X² = 556.167, df = 72, P < 0.001) and larvae (X² = 633.11, df = 66, P < 0.001) were significantly different. There was a significant difference among the bacterial communities between field-collected adults (X² = 48.974, df = 10, P < 0.001) and larvae (X² = 84.981, df = 10, P < 0.001). Lysinibacillus sphaericus was a common species in adults and larvae of laboratory-reared Ae. aegypti. Only P. dispersa occurred in the field-collected adults of Ae. aegypti and Ae. albopictus. Species belonging to genera Terribacillus, Lysinibacillus, Agromyces and Kocuria were recorded from Aedes mosquitoes, in accordance with previously reported results. Conclusions: This study generated a comprehensive database on the culturable bacterial community found in the midgut of field-collected (Ae. aegypti and Ae. albopictus) and laboratory-reared (Ae. aegypti) mosquito larvae and adults from Sri Lanka. Data confirm that the midgut bacterial diversity in the studied mosquitoes varies according to species, developmental stage and strain (field vs laboratory). Graphical