The genus Eupatorium belongs to the Asteraceae (Compositae) family and has multiple properties, such as invasiveness and toxicity, and is used in folk medicine. The last review on the chemical constituents of this genus and their biological activities was published in 2015. The present review provides an overview of 192 natural products discovered from 2015 to the present. These products include 63 sesquiterpenoids, 53 benzofuran derivatives, 39 thymol derivatives, 15 fatty acids, 7 diterpenoids, 5 monoterpenoids, 4 acetophenones, and 6 other compounds. We also characterized their respective chemical structures and cytotoxic, antifungal, insecticidal, antibacterial, anti-inflammatory, and antinociceptive activities.

Herein, the chemical analysis of petroleum ether extract of Eupatorium adenophorum was conducted which led to the identification of a previously unreported sesquiterpenoid, named eupatorione A (1). The structure of eupatorione A (1) was elucidated through comprehensive spectroscopic analysis, including 1D, 2D-NMR techniques, as well as HRMS. Additionally, X-ray diffraction was employed to further confirm the structure. The anti-inflammatory activity of compound 1 was assessed by measuring its inhibitory effects on the production of nitric oxide (NO), induced by lipopolysaccharide (LPS) in RAW264.7 LPS-activated macrophages.

Ten sesquiterpenoids (1-10), including two new compounds 1 and 2, were isolated with the use of several chromatographic methods from the above-ground tissues of Eupatorium adenophorum Spreng. Comprehensive spectroscopic studies, such as single-crystal X-ray diffraction, 1D, 2D-NMR, and HRMS, were used to characterize their structures. The cytotoxic and anti-inflammatory actions and properties of the new compounds 1 and 2 were examined and briefly discussed.

Chemical analysis of the aerial parts of Artemisia argyi H. Lév. & Vaniot led to the isolation of 6 lignans, including a new lignan glycoside, artemisiaside A, using various chromatographic techniques. Detailed spectroscopic (including 1D, 2D- nuclear magnetic resonance) and high resolution mass spectroscopy procedures, and electronic circular dichroism were used to ascertain the structural orientations of these compounds. The anti-inflammatory activities of compounds 1 to 6 were evaluated by measuring their inhibitory effects on lipopolysaccharide (LPS) -induced nitric oxide (NO) production in RAW264.7 LPS-activated macrophages. At 50 μM, compound 1 showed moderate anti-inflammatory activity with an inhibition rate of 61.2%.