Nitrogen is one of the most important nutrient for plant growth and development; it is strongly associated with a variety of abiotic stress responses. As sessile organisms, plants have evolved to develop efficient strategies to manage N to support growth when exposed to a diverse range of stressors. This review summarizes the recent progress in the field of plant nitrate (NO₃^-) and ammonium (NH₄⁺) uptake, which are the two major forms of N that are absorbed by plants. We explore the intricate relationship between NO₃^-/NH₄⁺ and abiotic stress responses in plants, focusing on stresses from nutrient deficiencies, unfavorable pH, ions, and drought. Although many molecular details remain unclear, research has revealed a number of core signaling regulators that are associated with N-mediated abiotic stress responses. An in-depth understanding and exploration of the molecular processes that underpin the interactions between N and abiotic stresses is useful in the design of effective strategies to improve crop growth, development, and productivity.

Protons (H⁺) in acidic soils arrest plant growth. However, the mechanisms by which plants optimize their biological processes to diminish the unfavorable effects of H⁺ stress remain largely unclear. Here, we showed that in the roots of Arabidopsis thaliana, the C2H2-type transcription factor STOP1 in the nucleus was enriched by low pH in a nitrate-independent manner, with the spatial expression pattern of NITRATE TRANSPORTER 1.1 (NRT1.1) established by low pH required the action of STOP1. Additionally, the nrt1.1 and stop1 mutants, as well as the nrt1.1 stop1 double mutant, had a similar hypersensitive phenotype to low pH, indicating that STOP1 and NRT1.1 function in the same pathway for H⁺ tolerance. Molecular assays revealed that STOP1 directly bound to the promoter of NRT1.1 to activate its transcription in response to low pH, thus upregulating its nitrate uptake. This action improved the nitrogen use efficiency (NUE) of plants and created a favorable rhizospheric pH for root growth by enhancing H⁺ depletion in the rhizosphere. Consequently, the constitutive expression of NRT1.1 in stop1 mutants abolished the hypersensitive phenotype to low pH. These results demonstrate that STOP1-NRT1.1 is a key module for plants to optimize NUE and ensure better plant growth in acidic media.

Increased soil salinity significantly inhibits crop production worldwide, and biochar may alleviate salt stress. In the present study, the application of biochar significantly increased the biomass of rice under salt stress treatment. The analysis of soil properties demonstrated that biochar application significantly decreased electrical conductivity and soluble Na⁺ and Cl⁻ contents in the soil under salt stress. In addition, biochar application increased the soil cation exchange capacity, soil organic matter, humic acid, total nitrogen, and total phosphorus contents in the soil, suggesting that biochar improved the soil nutrient conditions. The application of biochar further increased the abundance of soil bacteria and changed the bacterial community structure under salt stress. Proteobacteria, Chloroflexi, and Acidobacteria were the top three phyla in bacterial abundance. Biochar increased Proteobacteria abundance and decreased Chloroflexi abundance, which were considered to be eutrophic bacteria and oligotrophic bacteria, respectively. Redundancy analysis showed that soil bacterial communities were mainly affected by soil pH and EC (p < 0.05). In conclusion, the application of biochar alleviated salt stress in rice via modifying soil properties and regulating the bacterial abundance and community structure.

NRT 1.1 has been previously characterized as a dual‐affinity nitrate transporter in Arabidopsis , though several lines of evidence have raised questions regarding its high‐affinity function in nitrate uptake. Here, we show that the induction of NRT 2.1‐ and NRT 2.2‐mediated nitrate uptake interferes with measurements of the contribution of NRT 1.1 to high‐affinity uptake using nrt1.1 mutants. Therefore, a nrt1.1/2.1/2.2 triple mutant was generated to reevaluate the role of NRT 1.1 in high‐affinity nitrate uptake. This triple mutant has a lower rate of nitrate uptake than the nrt2.1/2.2 double mutant under low external nitrate supply, resulting in a lower growth rate than that of the double mutant. Therefore, we conclude that NRT 1.1‐mediated high‐affinity nitrate uptake is necessary for plant growth under low‐nitrate conditions.