The interaction between cytokinin, auxin and GA controlled in vitro shoot multiplication in paulownia was influenced by a medium water potential (Ψ) modulation, where it was modulated using different textures or strengths of MS medium, media of different types (MS, WPM, SH and B5) or NaCl incorporation. The interaction between 2 mg/L BAP and 0.1 mg/L NAA expressed the highest shoot number on each media type, but it was better with media of lower water potential (MS and WPM), and MS medium was the best. Ψ of full-strength semisolid MS medium expressed the highest shoot multiplication. The opposite was detected when Ψ of MS medium was changed using half- or double-strength MS. Ψ of full-strength MS medium in semisolid form resulted in a valuable interaction between 2 mg/L BAP, 0.1 mg/L NAA and 0.1 mg/L GA, leading to efficient shoot formation, and it was associated with an increase in internode length and decrease in stem diameter, which facilitated obtaining synseeds with a high ability to convert. High genetic variation was recorded under long-term culture (14 subcultures). Polymorphism using the ISSR technique was higher than that of RAPD. A further increase in polymorphism was detected when NaCl was used, where five salt-tolerant lines were selected. Some salt-tolerant-selected lines showed one or more amplification products of a specific molecular weight that did not appear in the control. For example, with OPA-07 and OPG-02 RAPD primers, all the salt-tolerant-selected lines showed the appearance of amplification fragments (610 bp and 300 bp, respectively) that were not detected in control.

Banana micropropagation is commercially established by the use of shoot tips obtained from intact plant on shoot multiplication medium under optimal culture conditions. However, this is somewhat associated with tissue browning, which may be associated with a reduced efficiency of shoot multiplication process. An increase or decrease in incubation temperature from 27 ºC, change the texture of the media from semisolid state, addition of ascorbic acid and substitution of optimal concentration of benzyl amino purine (5 mg/l BAP) with 2-isopentyl adenine (4 mg/l 2ip), decreased shoot multiplication and growth, even if they reduced tissue browning. Our results indicated that the chemical composition of the MS medium containing 5 mg/l BAP was ideal for banana shoot multiplication. Addition of some natural ingredients rich in many important components (yeast extract, coconut endosperm milk or moringa leaf extract) was unsuitable for in vitro multiplication of banana. The observed exception was that growth parameters of shoots formed on MS medium with 4 mg/l 2ip were better than those formed on MS with 5 mg/l BAP. In addition, shoot multiplication on 2-isopentenyl adenine (2ip) containing media showed root formation, which facilitated the adaptation and transfer of plants to soil.

Urinary tract infection is considered a serious public health problem. One hundred and fifty patients were included in the present study and suspected to have community-acquired urinary tract infections depending on crucial indicators. Out of 150 urine cultures, 69.3% were confirmed as urinary tract infection with significant growth and 30.7% was sterile pyuria. Community-acquired urinary tract infections showed high incidence in females (44.1%), followed by kids (32.3%; 19.6% girls and 12.7% boys), and males (23.5%) with differences regarding age groups. Gram staining indicated that 63.2% of total isolated uropathogens were gram-negative isolates, and 36.7% were gram-positive isolates. The identification of 106 bacterial isolates led to the presence of 16 distinct species belonging to 12 genera including Escherichia coli (40.56%), Enterococcus spp. (18.86%), Streptococcus spp. (10.37%), Klebsiella spp. (8.49%), Citrobacter koseri (6.60%), Corynebacterium urealyticum (4.71%), Proteus mirabilis (2.83%), Enterobacter intermedius (1.88%), Pseudomonas aeruginosa (1.88%), Staphylococcus saprophyticus (1.88%), Serratia fonticola (0.94%) and Bacillus cereus (0.94%). Phenotypic detection of ESBL revealed that 35.8% of total isolated gram-negative uropathogens were ESBL-producers. Escherichia coli comprised a serious threat as the most dominant ESBL-producing organism representing 66.7% of total ESBL-producing bacteria detected followed by Klebsiella pneumonia (12.5%), Citrobacter koseri (8.3%), Serratia fonticola (4.1%), Proteus mirabilis (4.1%), and Pseudomonas aeruginosa (4.1%).