To further explore characteristics of myopia and changes in factors associated with myopia among students at Inner Mongolia Medical University. Two cross-sectional censuses were conducted in 2011 and 2013. Participants were medical students residing on campus in 2011 and 2013. Logistic regression analysis was performed to ascertain associations with basic information, genetic factors, environmental factors. The χ2 test was used to test for differences in prevalence between 2011 and 2013. Prevalence was calculated at various myopia occurrence times among different parental myopia statuses. A total of 11,138 students enrolled from 2007 to 2012 completed the questionnaire. The prevalence of myopia in 2011 and 2013 was 70.50% and 69.21%, respectively, no statistically significant difference existed between the two censuses (p = 0.12). Both censuses were completed by 1015 students. There were no differences among the various year of study in 2011 or 2013. Myopic prevalence increased with an increased number of myopic parents: the prevalence if both parents were myopic was over 90%, nearly 80% if one parent was myopic, and less than 70% with non-myopic parents (p < 0.001). Myopic occurrence ranked from earliest to latest was in kindergarten and primary school when both parents were myopic, in middle school when one parent was myopic, and in university when no parent was myopic. Students staying up late, using a computer more than 3 h per day, not performing eye exercises, using eye drops, and rubbing the eyes at high risk for myopia. Myopic status was stable during the university period. Genetic factors play a major role in myopia. Protective measures are useful for university students.

Background: Gastric cancer (GC) remains a malignant tumor with high morbidity and mortality, accounting for approximately 1,080,000 diagnosed cases and 770,000 deaths worldwide annually. Disulfidptosis, characterized by the stress-induced abnormal accumulation of disulfide, is a recently identified form of programmed cell death. Substantial studies have demonstrated the significant influence of immune clearance on tumor progression. Therefore, we aimed to explore the intrinsic correlations between disulfidptosis and immune-related genes (IRGs) in GC, as well as the potential value of disulfidptosis-related immune genes (DRIGs) as biomarkers. Methods: This study incorporated the single-cell RNA sequencing (scRNA-seq) dataset GSE183904 and transcriptome RNA sequencing of GC from the TCGA database. Disulfidptosis-related genes (DRGs) and IRGs were derived from the representative literature on both cell disulfidptosis and immunity. The expression and distribution of DRGs were investigated at the single-cell level in different GC cell types. Pearson correlation analysis was used to identify the IRGs closely related to disulfidptosis. The prognostic signature of DRIGs was established using Cox and LASSO analyses. We then analyzed and evaluated the differences in long-term prognosis, Gene Set Enrichment Analysis (GSEA), immune infiltration, mutation profile, CD274 expression, and response to chemotherapeutic drugs between the two groups. A tissue array containing 63 paired GC specimens was used to verify the expression of 4 DRIGs and disulfidptosis regulator SLC7A11 through immunohistochemistry staining. Results: The scRNA-seq analysis found that SLC7A11, SLC3A2, RPN1 and NCKAP1 were enriched in specific cell types and closely related to immune infiltration. Four DIRGs (GLA, HIF-1α, VPS35 and CDC37) were successfully identified to establish a signature to potently predict the survival time of GC patients. Patients with high risk scores generally experienced worse prognoses and exhibited greater resistant to classical chemotherapy drugs. Furthermore, the expression of GLA, HIF-1α, VPS35, CDC37 and SLC7A11 were elevated in GC tissues. A high expression of GLA, HIF-1α, VPS35 or CDC37 was associated with more advanced clinical stage of GC and increased SLC7A11 expression. Conclusion: Current study first highlights the potential value of DRIGs as biomarkers in GC. We successfully constructed a robust model incorporating four DRIGs to accurately predict the survival time and clinicopathological characteristics of GC patients.

Background: This study aims to investigate the effects of water soluble particulate matter (WSPM) on the viability and protein expression profile of human lung adenocarcinoma cell A549 in the Bayou Obo rare earth mining area, and explore the influence of WSPM on the A549 cell cycle.Results: It was found that WSPM can inhibit the viability of A549 cells and induce cell arrest in the G2/M phase. Compared with controls, exposure to WSPM10 and WSPM2.5 induced 134 and 116 proteins to be differentially expressed in A549 cells, respectively. In addition, 33 and 31 differentially expressed proteins were further confirmed, and was consistent with the proteomic analysis. The most prominent enrichment in ribosome-associated proteins were presented. WhenRPL6,RPL13, orRPL18Agene expression was inhibited, A549 cells were arrested in the G1 phase, affecting the expression ofCyclin D1,p21,RB1,Cyclin A2,Cyclin B1,CDC25A,CDK2,CHEK2andE₂F₁. Furthermore, the La³⁺, Ce³⁺, Nd³⁺and F^-in WSPM also inhibited the viability of A549 cells. After 24 h of exposure to 2 mM of NaF, A549 cells were also arrested in the G2/M phase, while the other three compounds did not have this effect. These four compounds affected the cell cycle regulatory factors in A549 cells, mainly focusing on effecting the expression ofCDK2,CDK4,RB1,ATM,TP53andMDM2genes. These results are consistent with the those from WSPM exposure.Conclusions: These results revealed that WSPM from rare earth mines decreased the viability of A549 cells, and induced cell cycle G2/M phase arrest, and even apoptosis, which may be independent of the NF-κB/MYD88 pathway, and be perceived by the TLR4 receptor. The dysfunction of the cell cycle is correlated to the down-expression of ribosomal proteins (RPs). However, it is not the direct reason for the A549 cell arrest in the G2/M phase. La³⁺, Ce³⁺, and F^-are probably the main toxic substances in WSPM, and may be regulate the A549 cell cycle by affecting the expression of genes, such asMDM2, RB1, ATM, TP53, E₂F₁,CDK2andCDK4. These results indicate the importance for further research into the relationship between APM and lung cancer.

Objectives A major goal of our study was to identify the associations between lifestyle factors and obesity in adolescents and young adults at risk by surveying students in Inner Mongolia Medical University. A second goal was to determine these factors differed by gender. Design Cross-sectional study. Setting Students grade 1–3 in Inner Mongolia Medical University. Participants 5471 grade 1–3 medical students, composed of 3891 female and 1580 male students. Students with body mass index (BMI) ≥25 were defined as overweight. Results BMI for male students was 22.1±2.9 and 21.2±2.2 for female students. The prevalence of overweight was 7.6%, with the prevalence being higher for male students compared with females, urban higher than rural and being an only child higher than having sibling children. For male students, urban residence was a risk factor, while for female students being an only child and staying up at night were risk factors, with physical activity a protective factor. A dose-dependency relationship was found between physical fitness and overweight prevalence. Conclusions This study shows that being an only child and resident in an urban area are risk factors; staying up late and lack of physical activities increased the risk of being overweight. BMI was associated with declines in physical fitness. Our study provides more insight into adolescent obesity problems.