During microbial-enhanced oil recovery (MEOR), surfactant-producing microorganisms are reported to improve displacement efficiency. However, the sweep efficiency could be improved by emulsified droplets or be reduced by low-IFT (interfacial tension)-induced fingering flow. Therefore, whether sweep efficiency can be improved by surfactant-producing microorganisms is currently unclear. To reveal the EOR mechanisms by surfactant-producing microorganisms, a 2D micro-model was used to conduct a long-term pore-scale experiment. In the results, 19.4% of the original oil in place (OOIP) was recovered, and surfactant-producing microorganisms can improve not only displacement efficiency (16.9% of the OOIP in the main stream) but also sweep efficiency (27.7% of the OOIP in the margin). Furthermore, the sweep efficiency was improved during flooding and shut-in periods. For instance, 19.5% of the OOIP in margins migrated to the main stream during the 1st shut-in period. Regarding mechanisms of sweep, it was improved by Jamin’s effect during the flooding period, while during the shut-in period, the oil migration was attributed to the spontaneously spreading biomass and their wettability altering the biosurfactant. This long-term experiment revealed that the dominant oil recovery mechanisms were altering with declining oil saturation, based on which sweep efficiency contributed to oil recovery only at oil saturation higher than 40.5%. While at lower oil saturation, only displacement efficiency could be improved.

The rheology of a partially hydrolyzed polyacrylamide (HPAM) solution plays an important role in its oil recovery during polymer flooding. However, multiple factors in brine, such as sulfide ions, cause a dramatic loss in the viscosity and oil recovery. To better understand the sulfide-induced viscosity loss and the consequent flow mechanisms in pore networks, the morphology of polymer solutions with and without sulfide ion was observed by scanning electron microscopy; and the variations of the pore scale flow fields were demonstrated by a microscopic visualization seepage experiment combined with Micro-PIV (Microscale Particle Image Velocimetry). The results showed that, with the presence of sulfide ion, the microstructure of the polymer changed from a uniform three-dimensional network structure to loose and uneven floccules, which resulted in viscosity loss (over 70% with 5-mg/L sulfide ion). Moreover, higher concentrations of sulfide ions (5 mg/L and 10 mg/L) resulted in earlier shear thinning characteristics than those with lower sulfide concentrations. Due to viscosity loss, the average flow velocity in the main stream of the microscopic seepage experiment increased more significantly than that without sulfide. However, the viscosity loss alone cannot independently explain the severe viscous fingering during the subsequent post-water flooding, which was about five times greater than that of the primary water flooding in terms of the velocity ratio between the mainstream and margin. A further pore-scale flow field analysis exhibited an eccentric and a bimodal velocity distribution in the throat along the radial and axial directions, respectively. The former distribution indicated that the adsorbed polymer on the pore wall was broken through by hydraulic shear due to the collapsed structure caused by sulfide ion. The latter suggested that another sulfide-induced impact was an earlier-occurring non-Newtonian characteristic with a low shear rate. Therefore, instead of viscosity loss, elastic loss is the dominant mechanism affecting the characteristics of the aggregate flow field under the action of sulfide. Microscopic flooding combined with Micro-PIV is a feasible and essential method to reveal pore scale flow mechanisms.