The preliminary structure, in vitro antioxidant and in vivo hepatoprotective activities of water-soluble polysaccharides (ORWP) and alkali-soluble polysaccharides (ORAP), prepared from the mushroom Oudemansiella radicata, were investigated. Both ORWP and ORAP were heteropolysaccharides with mannose, glucose and galactose being the main monosaccharide components. Regarding the antioxidant activities, ORWP and ORAP showed effective 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, hydrogen peroxide scavenging activity and lipid peroxidation inhibitory effects, as well as moderate reducing power and Fe²⁺ chelating activity. For the hepatoprotective activity, administration of ORWP and ORAP prevented the increase in serum alanine aminotransferase and aspartate aminotransferase activities in a carbon tetrachloride-induced acute liver damage model, suppressed hepatic malondialdehyde formation and stimulated the activities of hepatic superoxide dismutase and glutathione peroxidase. Thus, we speculate that ORWP and ORAP may protect the liver from CCl₄-induced hepatic damage via antioxidant mechanisms.

To date, only a few steroids have been isolated from the mushroom Stropharia rugosoannulata which can be cultivated. In this paper, a novel lectin (SRL) with a molecular weight of 38 kDa, and a unique IKSGVYRIVSWQGALGPEAR N-terminal sequence was isolated from S. rugosoannulata, which represents the first protein isolated from the mushroom. The purification methods included (NH₄)₂SO₄ precipitation, ion exchange chromatography on CM-cellulose, Q-Sepharose, and SP-Sepharose, and gel- filtration on Superdex-75. The lectin was adsorbed on all three types of ion exchangers and was purified more than 450-fold. The lectin was stable below 70 °C (with half of the activity preserved at 80 °C), and in the presence of NaOH and HCl solutions up to a concentration of 12.5 mM and 25 mM, respectively. The hemagglutinating activity of SRL was inhibited by inulin. Cd²⁺ and Hg²⁺ ions strongly reduced the hemagglutinating activity at concentrations from 1.25 mM to 10 mM. SRL exhibited anti-proliferative activity toward both hepatoma Hep G2 cells and leukemia L1210 cells, with an IC₅₀ of 7 μM and 19 μM, respectively. The activity of HIV-1 reverse transcriptase could also be inhibited by SRL, with an IC₅₀ of 10 μM.

An acidic α-galactosidase designated as TMG was purified from the fruiting bodies The purification protocol entailed ion exchange chromatography on Q-Sepharose and of Tricholoma matsutake with 136-fold purification and a specific activity of 909 units/mg. Mono-Q and fast protein liquid chromatography on Superdex 75. TMG is a monomeric protein exhibiting a molecular mass of 47 kDa in SDS-PAGE and gel filtration. The purified enzyme was identified by LC-MS/MS and three inner amino acid sequences were obtained. The optimum pH and temperature for TMG with pNPGal as substrate were pH 4.5 and 55 °C, respectively. The α-galactosidase activity was strongly inhibited by K⁺, Ca²⁺, Cd²⁺, Hg²⁺, Ag⁺ and Zn²⁺ ions. The enzyme activity was inhibited by the chemical modification agent N-bromosuccinimide (NBS), indicating the importance of tryptophan residue(s) at or near the active site. Besides hydrolyzing pNPGal, TMG also efficaciously catalyzed the degradation of natural substrates such as stachyose, raffinose, and melibiose. Thus TMG can be exploited commercially for improving the nutritional value of soy milk by degradation of indigestible oligosaccharides.

An 86-kDa homodimeric angiotensin I-converting enzyme (ACE) inhibitory protein designated as LTP was isolated from fruit bodies of the mushroom Leucopaxillus tricolor. The isolation procedure involved ultrafiltration through a membrane with a molecular weight cutoff of 10-kDa, ion exchange chromatography on Q-Sepharose, and finally fast protein liquid chromatography-gel filtration on Superdex 75. LTP exhibited an IC₅₀ value of 1.64 mg∙mL⁻¹ for its ACE inhibitory activity. The unique N-terminal amino acid sequence of LTP was disclosed by Edman degradation to be DGPTMHRQAVADFKQ. In addition, seven internal sequences of LTP were elucidated by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Results of the Lineweaver-Burk plot suggested that LTP competitively inhibited ACE. Both LTP and the water extract of L. tricolor exhibited a clear antihypertensive effect on spontaneously hypertensive rats.

Pleurotus ostreatus is the third largest cultivated species in China’s edible mushroom industry; however, its agricultural cultivation method is easily affected by high-temperature environments. To understand the response mechanism of mycelia to heat stress, the mycelia of P. ostreatus, which had been grown at 28 °C for 4 days, were subjected to heat stress at 32 °C and 36 °C for 2 days, followed by RNA-seq analysis. These results indicate that, under heat stress, mycelial growth was significantly inhibited, the cell membrane was disrupted, the cell walls became thicker, and chitinase and β-1,3-glucanase activities decreased. Transcriptome analysis revealed 2118 differentially expressed genes (DEGs) under 36 °C heat stress, and 458 DEGs were identified under 32 °C heat stress. A total of 328 DEGs were upregulated or downregulated under heat stress at 36 °C and 32 °C. The functional enrichment analysis of these genes revealed significant enrichment in genes related to hydrogen peroxide metabolism, oxidoreductase activity, ATP hydrolysis, and cell wall structure composition. There was a total of 80 DEGs specific to heat stress at 32 °C, and they were significantly enriched in catalase activity, the cell wall, the aminoglycan catabolic process, and oxidoreductase activity. However, 817 DEGs specific to heat stress at 36 °C were significantly enriched in the cell wall, integral components of the membrane, and oxidoreductase activity. The identification of cell wall-related genes revealed that hydrophobic proteins, Cerato plateau proteins, laccases, and glycoside hydrolases may respond to stress. The results of qRT-PCR for cell wall-related genes are consistent with the RNA-seq data. This study revealed several potential candidate genes for high-temperature thermal response, laying the foundation for the study of the thermal response mechanism of P. ostreatus.