Tomato brown rugose fruit virus (ToBRFV) is an emerging pathogen affecting tomato-production systems in several countries, including Mexico. This situation involves challenges due to the negative impact on yield and the lack of disease-management measures. This work analyzes the spatiotemporal distribution of ToBRFV in commercial tomato greenhouses. The presence or absence of diseased plants was evaluated weekly, assigning a location in space (x, y). Temporal analysis consisted of fitting the incidence to the monomolecular, logistic, log-logistic, Gompertz, exponential, Weibull, and Richard models, evaluated using the Akaike information criterion, significance, correlation, coefficient of determination, and root mean square error. Spatial analysis consisted of determining spatial aggregation using the Moran, Fisher, and Lloyd indices. In addition, spatial distribution was assessed by sequence observations, point patterns using the inverse distance index, and analysis by SADIE distance indicators. Results indicated that the logistic models (log-logistic and logistic) best described the temporal progress of ToBRFV. This disease also had slightly aggregated patterns in the initial phase, highly aggregated in the exponential phase, and uniform in the deceleration and stationary phases. This study demonstrates that the spatial and temporal dynamics of ToBRFV have important implications for the monitoring, diagnosis, management, and risk prediction of this disease.

Basal stem rot (BSR), caused by Ganoderma spp., is one of the most important emerging oil palm diseases in Colombia, and is restricted to two oil palm production areas in the country. To identify the causal agent of the disease, basidiocarp of oil palms affected by BSR were used to prepare isolates, and their pathogenicity was then assessed in pre-nursery plants. Four-month-old oil palm seedlings were inoculated with rubber wood (Hevea brasiliensis) blocks colonized with dikaryotic mycelia of Ganoderma. The incidence, severity, and symptoms of the pathogen were assessed. A multiregional analysis (ITS, rpb2, and tef1-α) was carried out to identify the isolates; all isolates were determined to be Ganoderma zonatum. Phylogenetic analyses with the three regions yielded concordant phylogenetic information and supported the distinction of the isolates with high bootstrap support. Seven isolates (CPBsZN-01-29, CPBsZN-02-30, CPBsZN-03-31, CPBsZN-04-34, CPBsZN-05-35, CPBsZN-06-36, and CPBsZN-07-38) were pathogenic in oil palm, with incidences greater than 90% and a maximum severity of 34%, and the highest severity index was found in isolates CPBsZN-03-31, CPBsZN-04-34, and CPBsZN-06-36. The pathogen was recovered from inoculated oil palms in all cases. This study reveals the pathogenic association of Ganoderma zonatum with BSR in Colombia.

The fruit agribusiness sector is one of the most dynamic in Colombia. Within this sector, avocado and mango are of great importance, both in terms of area planted and volume exported. Increasing the productivity, quality, and postharvest life of these fruits is a priority, due to the high losses that occur in the preharvest and postharvest stages. One current alternative to achieve this are biostimulants, which have the potential to improve the productivity and quality of fruit. The objective of this work was to evaluate the effect of the preharvest application of two biostimulants on the yield and quality of avocados and mangos. The study was carried out in production systems for cv. Hass avocado (traditional and organic) and cv. Keitt mango (traditional). In each system, two treatments were evaluated: conventional and conventional + biostimulant. Under field conditions, yield and quality variables such as weight, number of fruits, visual appearance, and size were determined. In postharvest, the fruits obtained from preharvest treatments were subjected to two storage conditions (6 and 20 °C) during the ripening process. Postharvest quality variables such as respiration, ethylene rates, weight loss, color index, firmness, total soluble solids, and titratable acidity were evaluated. The results indicated that the use of biostimulants caused a significant increase (p ≤ 0.05) in the yield, number of fruits, and preharvest quality parameters of size and weight, without negatively affecting the postharvest quality of the fruits. The biostimulant increases the total weight of fruits in organic and conventional avocado by ~55 and 25%, respectively, while in mango, this figure increased by ~23%. Hence, biostimulants can be utilized to increase the productivity of fruit trees.

Reader Q: There was no morphological information provided for the isolated specimens, and so it is not known if they are even Ganoderma