The aim of this study was to compare the effects of different farming systems (organic, integrated, conventional and monoculture) on some soil properties as: bulk density, contents of readily-dispersible clay, organic matter and particulate organic matter, and enzymatic activity measured in terms of the intensity of fluorescein diacetate hydrolysis. Soil under permanent grass was used as a control. The study was conducted on the 20 years lasting field experiment. Samples of Haplic Luvisol soil were collected twice a year on fields under winter wheat from the layers of 0-5, 5-10, 15-20, and 30-35 cm. Within arable soils the soil under organic farming contained the greatest amount of organic matter, which influenced strongly the readily-dispersible clay content, especially in the layer of 5-20 cm. The readily-dispersible clay content in soil under organic farming was 3 times lower, as compared to the conventional and monoculture farming. The highest contents of particulate organic matter 6.2 and 3.5 mg g⁻¹air dry soil, on average were measured in the 0-5 cm layer of control soil and soil under organic farming, respectively. Also, soil under organic farming and control soil from the depth of 0-5 cm showed 2-2.5 times greater activity of microorganisms in fluorescein diacetate hydrolysis than soil under conventional and monoculture farming. Increase of concentration of organic matter in soil under organic farming decreased soil bulk density. Statistical analysis showed significant correlations between studied parameters of soil quality and confirmed their effectiveness as indicators of disturbances in soil environment.